Experiment: lambda1857_phage_12.5_MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECD_00330 and ECD_00331 are separated by 118 nucleotides ECD_00331 and ECD_00332 are separated by 101 nucleotides ECD_00332 and ECD_00333 are separated by 16 nucleotides ECD_00333 and ECD_00334 are separated by 119 nucleotides
ECD_00330: ECD_00330 - bacterial alkaline phosphatase, at 366,372 to 367,787
_00330
ECD_00331: ECD_00331 - PsiF family protein, at 367,906 to 368,226
_00331
ECD_00332: ECD_00332 - diguanylate cyclase, cellulose regualtor, at 368,328 to 369,443
_00332
ECD_00333: ECD_00333 - pyrroline-5-carboxylate reductase, NAD(P)-binding, at 369,460 to 370,269
_00333
ECD_00334: ECD_00334 - UPF0178 family protein, at 370,389 to 370,847
_00334
Position (kb) 368
369
370 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 367.532 kb on - strand, within ECD_00330 at 367.532 kb on - strand, within ECD_00330 at 367.611 kb on - strand, within ECD_00330 at 367.641 kb on + strand, within ECD_00330 at 367.641 kb on + strand, within ECD_00330 at 367.755 kb on + strand at 367.756 kb on - strand at 367.849 kb on + strand at 367.906 kb on - strand at 367.926 kb on + strand at 367.926 kb on + strand at 367.926 kb on + strand at 367.927 kb on - strand at 368.001 kb on + strand, within ECD_00331 at 368.002 kb on - strand, within ECD_00331 at 368.002 kb on - strand, within ECD_00331 at 368.002 kb on - strand, within ECD_00331 at 368.062 kb on + strand, within ECD_00331 at 368.063 kb on - strand, within ECD_00331 at 368.146 kb on - strand, within ECD_00331 at 368.187 kb on + strand, within ECD_00331 at 368.196 kb on + strand at 368.197 kb on - strand at 368.197 kb on - strand at 368.305 kb on + strand at 368.306 kb on - strand at 368.575 kb on + strand, within ECD_00332 at 368.613 kb on - strand, within ECD_00332 at 368.633 kb on - strand, within ECD_00332 at 368.714 kb on + strand, within ECD_00332 at 368.714 kb on + strand, within ECD_00332 at 368.867 kb on + strand, within ECD_00332 at 368.878 kb on - strand, within ECD_00332 at 368.982 kb on + strand, within ECD_00332 at 368.983 kb on - strand, within ECD_00332 at 368.983 kb on - strand, within ECD_00332 at 369.005 kb on + strand, within ECD_00332 at 369.005 kb on + strand, within ECD_00332 at 369.005 kb on + strand, within ECD_00332 at 369.006 kb on - strand, within ECD_00332 at 369.396 kb on + strand at 369.589 kb on - strand, within ECD_00333 at 369.589 kb on - strand, within ECD_00333 at 369.589 kb on - strand, within ECD_00333 at 369.589 kb on - strand, within ECD_00333 at 369.647 kb on - strand, within ECD_00333 at 369.729 kb on + strand, within ECD_00333 at 369.729 kb on + strand, within ECD_00333 at 369.729 kb on + strand, within ECD_00333 at 369.730 kb on - strand, within ECD_00333 at 369.730 kb on - strand, within ECD_00333 at 369.737 kb on + strand, within ECD_00333 at 369.793 kb on + strand, within ECD_00333 at 369.823 kb on - strand, within ECD_00333 at 370.006 kb on - strand, within ECD_00333 at 370.006 kb on - strand, within ECD_00333 at 370.166 kb on + strand, within ECD_00333 at 370.167 kb on - strand, within ECD_00333 at 370.167 kb on - strand, within ECD_00333 at 370.249 kb on - strand at 370.322 kb on + strand at 370.322 kb on + strand at 370.332 kb on + strand at 370.389 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction lambda1857_phage_12.5_MOI remove 367,532 - ECD_00330 0.82 +1.7 367,532 - ECD_00330 0.82 +1.2 367,611 - ECD_00330 0.88 -3.0 367,641 + ECD_00330 0.90 -0.1 367,641 + ECD_00330 0.90 +0.9 367,755 + -1.1 367,756 - -2.4 367,849 + -0.1 367,906 - -0.9 367,926 + -2.2 367,926 + -1.7 367,926 + -2.7 367,927 - -2.1 368,001 + ECD_00331 0.30 +0.4 368,002 - ECD_00331 0.30 +0.5 368,002 - ECD_00331 0.30 -2.8 368,002 - ECD_00331 0.30 +0.6 368,062 + ECD_00331 0.49 +2.6 368,063 - ECD_00331 0.49 +1.5 368,146 - ECD_00331 0.75 +1.3 368,187 + ECD_00331 0.88 +0.2 368,196 + -1.4 368,197 - +0.5 368,197 - -1.4 368,305 + +0.8 368,306 - +0.6 368,575 + ECD_00332 0.22 +0.8 368,613 - ECD_00332 0.26 +1.0 368,633 - ECD_00332 0.27 +1.7 368,714 + ECD_00332 0.35 +0.5 368,714 + ECD_00332 0.35 -2.9 368,867 + ECD_00332 0.48 -0.5 368,878 - ECD_00332 0.49 -1.5 368,982 + ECD_00332 0.59 -0.4 368,983 - ECD_00332 0.59 -1.3 368,983 - ECD_00332 0.59 +0.9 369,005 + ECD_00332 0.61 +0.4 369,005 + ECD_00332 0.61 +1.6 369,005 + ECD_00332 0.61 +0.7 369,006 - ECD_00332 0.61 +0.9 369,396 + +0.9 369,589 - ECD_00333 0.16 +0.6 369,589 - ECD_00333 0.16 -1.1 369,589 - ECD_00333 0.16 -1.4 369,589 - ECD_00333 0.16 +0.2 369,647 - ECD_00333 0.23 +0.5 369,729 + ECD_00333 0.33 -0.8 369,729 + ECD_00333 0.33 -1.1 369,729 + ECD_00333 0.33 -0.2 369,730 - ECD_00333 0.33 -1.0 369,730 - ECD_00333 0.33 -0.2 369,737 + ECD_00333 0.34 -2.2 369,793 + ECD_00333 0.41 +0.7 369,823 - ECD_00333 0.45 -0.2 370,006 - ECD_00333 0.67 +2.8 370,006 - ECD_00333 0.67 -1.8 370,166 + ECD_00333 0.87 -2.8 370,167 - ECD_00333 0.87 +0.3 370,167 - ECD_00333 0.87 +0.2 370,249 - -0.6 370,322 + -2.1 370,322 + -0.5 370,332 + +0.8 370,389 - +0.6
Or see this region's nucleotide sequence