Experiment: lambda1857_phage_12.5_MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECD_00293 and ECD_00294 are separated by 30 nucleotides ECD_00294 and ECD_00295 are separated by 32 nucleotides ECD_00295 and ECD_00296 are separated by 102 nucleotides ECD_00296 and ECD_00297 are separated by 65 nucleotides
ECD_00293: ECD_00293 - carbonic anhydrase, at 328,334 to 328,993
_00293
ECD_00294: ECD_00294 - cyanate aminohydrolase, at 329,024 to 329,494
_00294
ECD_00295: ECD_00295 - putative cyanate transporter, at 329,527 to 330,681
_00295
ECD_00296: ECD_00296 - thiogalactoside acetyltransferase, at 330,784 to 331,395
_00296
ECD_00297: ECD_00297 - lactose permease, at 331,461 to 332,714
_00297
Position (kb) 329
330
331 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 328.581 kb on - strand, within ECD_00293 at 328.678 kb on + strand, within ECD_00293 at 328.678 kb on + strand, within ECD_00293 at 328.679 kb on - strand, within ECD_00293 at 328.780 kb on - strand, within ECD_00293 at 328.807 kb on + strand, within ECD_00293 at 328.878 kb on - strand, within ECD_00293 at 328.998 kb on - strand at 329.241 kb on + strand, within ECD_00294 at 329.242 kb on - strand, within ECD_00294 at 329.306 kb on + strand, within ECD_00294 at 329.307 kb on - strand, within ECD_00294 at 329.333 kb on + strand, within ECD_00294 at 329.339 kb on - strand, within ECD_00294 at 329.383 kb on + strand, within ECD_00294 at 329.384 kb on - strand, within ECD_00294 at 329.414 kb on - strand, within ECD_00294 at 329.460 kb on + strand at 329.460 kb on + strand at 329.460 kb on + strand at 329.461 kb on - strand at 329.461 kb on - strand at 329.467 kb on + strand at 329.537 kb on + strand at 329.556 kb on + strand at 329.560 kb on - strand at 329.560 kb on - strand at 329.628 kb on + strand at 330.104 kb on + strand, within ECD_00295 at 330.136 kb on + strand, within ECD_00295 at 330.193 kb on + strand, within ECD_00295 at 330.194 kb on - strand, within ECD_00295 at 330.194 kb on - strand, within ECD_00295 at 330.194 kb on - strand, within ECD_00295 at 330.234 kb on - strand, within ECD_00295 at 330.285 kb on + strand, within ECD_00295 at 330.286 kb on - strand, within ECD_00295 at 330.318 kb on - strand, within ECD_00295 at 330.336 kb on - strand, within ECD_00295 at 330.336 kb on - strand, within ECD_00295 at 330.336 kb on - strand, within ECD_00295 at 330.558 kb on + strand, within ECD_00295 at 330.575 kb on - strand at 330.729 kb on - strand at 330.729 kb on - strand at 330.765 kb on - strand at 330.957 kb on + strand, within ECD_00296 at 331.013 kb on + strand, within ECD_00296 at 331.013 kb on + strand, within ECD_00296 at 331.163 kb on - strand, within ECD_00296 at 331.287 kb on + strand, within ECD_00296 at 331.570 kb on + strand at 331.601 kb on + strand, within ECD_00297 at 331.602 kb on - strand, within ECD_00297 at 331.602 kb on - strand, within ECD_00297 at 331.623 kb on - strand, within ECD_00297
Per-strain Table
Position Strand Gene LocusTag Fraction lambda1857_phage_12.5_MOI remove 328,581 - ECD_00293 0.37 -2.4 328,678 + ECD_00293 0.52 +0.7 328,678 + ECD_00293 0.52 +0.9 328,679 - ECD_00293 0.52 +1.5 328,780 - ECD_00293 0.68 -0.8 328,807 + ECD_00293 0.72 +2.3 328,878 - ECD_00293 0.82 -1.9 328,998 - +0.1 329,241 + ECD_00294 0.46 +1.4 329,242 - ECD_00294 0.46 +0.6 329,306 + ECD_00294 0.60 -0.1 329,307 - ECD_00294 0.60 +0.9 329,333 + ECD_00294 0.66 +0.8 329,339 - ECD_00294 0.67 -0.6 329,383 + ECD_00294 0.76 +1.8 329,384 - ECD_00294 0.76 -1.4 329,414 - ECD_00294 0.83 +1.5 329,460 + -0.3 329,460 + +1.0 329,460 + +1.4 329,461 - +1.6 329,461 - +0.3 329,467 + -0.0 329,537 + -0.0 329,556 + +3.0 329,560 - -0.9 329,560 - -0.9 329,628 + -1.2 330,104 + ECD_00295 0.50 +2.3 330,136 + ECD_00295 0.53 +0.7 330,193 + ECD_00295 0.58 -0.1 330,194 - ECD_00295 0.58 -1.4 330,194 - ECD_00295 0.58 +0.2 330,194 - ECD_00295 0.58 -1.1 330,234 - ECD_00295 0.61 +1.1 330,285 + ECD_00295 0.66 -0.1 330,286 - ECD_00295 0.66 +0.7 330,318 - ECD_00295 0.68 -0.4 330,336 - ECD_00295 0.70 +0.6 330,336 - ECD_00295 0.70 -0.2 330,336 - ECD_00295 0.70 -0.0 330,558 + ECD_00295 0.89 +0.0 330,575 - +0.2 330,729 - +3.2 330,729 - -2.4 330,765 - -0.1 330,957 + ECD_00296 0.28 -2.1 331,013 + ECD_00296 0.37 +0.2 331,013 + ECD_00296 0.37 -2.4 331,163 - ECD_00296 0.62 -1.7 331,287 + ECD_00296 0.82 -0.1 331,570 + +1.6 331,601 + ECD_00297 0.11 -0.1 331,602 - ECD_00297 0.11 +1.1 331,602 - ECD_00297 0.11 +1.2 331,623 - ECD_00297 0.13 -2.4
Or see this region's nucleotide sequence