Strain Fitness in Escherichia coli BL21 around ECD_00073

Experiment: lambda1857_phage_12.5_MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00072 and ECD_00073 are separated by 48 nucleotidesECD_00073 and ECD_00074 are separated by 10 nucleotides ECD_00072: ECD_00072 - broad specificity sugar efflux system, at 80,426 to 81,604 _00072 ECD_00073: ECD_00073 - 3-isopropylmalate dehydratase small subunit, at 81,653 to 82,258 _00073 ECD_00074: ECD_00074 - 3-isopropylmalate dehydratase large subunit, at 82,269 to 83,669 _00074 Position (kb) 81 82 83Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 80.675 kb on - strand, within ECD_00072at 80.676 kb on + strand, within ECD_00072at 80.709 kb on + strand, within ECD_00072at 80.722 kb on + strand, within ECD_00072at 80.732 kb on + strand, within ECD_00072at 80.796 kb on + strand, within ECD_00072at 80.935 kb on + strand, within ECD_00072at 80.936 kb on - strand, within ECD_00072at 80.995 kb on + strand, within ECD_00072at 80.996 kb on - strand, within ECD_00072at 81.042 kb on + strand, within ECD_00072at 81.042 kb on + strand, within ECD_00072at 81.067 kb on + strand, within ECD_00072at 81.070 kb on + strand, within ECD_00072at 81.075 kb on + strand, within ECD_00072at 81.075 kb on + strand, within ECD_00072at 81.075 kb on + strand, within ECD_00072at 81.085 kb on - strand, within ECD_00072at 81.125 kb on + strand, within ECD_00072at 81.125 kb on + strand, within ECD_00072at 81.125 kb on + strand, within ECD_00072at 81.125 kb on + strand, within ECD_00072at 81.125 kb on + strand, within ECD_00072at 81.126 kb on - strand, within ECD_00072at 81.126 kb on - strand, within ECD_00072at 81.126 kb on - strand, within ECD_00072at 81.130 kb on + strand, within ECD_00072at 81.130 kb on + strand, within ECD_00072at 81.136 kb on + strand, within ECD_00072at 81.245 kb on + strand, within ECD_00072at 81.245 kb on + strand, within ECD_00072at 81.246 kb on - strand, within ECD_00072at 81.282 kb on + strand, within ECD_00072at 81.309 kb on - strand, within ECD_00072at 81.373 kb on - strand, within ECD_00072at 81.405 kb on + strand, within ECD_00072at 81.405 kb on + strand, within ECD_00072at 81.406 kb on - strand, within ECD_00072at 81.454 kb on - strand, within ECD_00072at 81.458 kb on - strand, within ECD_00072at 81.466 kb on + strand, within ECD_00072at 81.467 kb on - strand, within ECD_00072at 81.539 kb on + strandat 81.655 kb on - strandat 81.715 kb on + strand, within ECD_00073at 81.919 kb on + strand, within ECD_00073at 81.964 kb on + strand, within ECD_00073at 81.965 kb on - strand, within ECD_00073at 82.275 kb on - strandat 82.525 kb on - strand, within ECD_00074at 82.696 kb on + strand, within ECD_00074at 82.696 kb on + strand, within ECD_00074at 82.696 kb on + strand, within ECD_00074at 82.696 kb on + strand, within ECD_00074at 82.696 kb on + strand, within ECD_00074at 82.696 kb on + strand, within ECD_00074at 82.697 kb on - strand, within ECD_00074at 82.697 kb on - strand, within ECD_00074at 82.760 kb on + strand, within ECD_00074at 82.949 kb on + strand, within ECD_00074at 82.949 kb on + strand, within ECD_00074at 82.949 kb on + strand, within ECD_00074at 82.949 kb on + strand, within ECD_00074at 82.949 kb on + strand, within ECD_00074at 82.950 kb on - strand, within ECD_00074at 82.950 kb on - strand, within ECD_00074at 82.950 kb on - strand, within ECD_00074at 83.021 kb on + strand, within ECD_00074at 83.037 kb on + strand, within ECD_00074at 83.038 kb on - strand, within ECD_00074at 83.038 kb on - strand, within ECD_00074at 83.038 kb on - strand, within ECD_00074at 83.135 kb on + strand, within ECD_00074at 83.214 kb on + strand, within ECD_00074

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Per-strain Table

Position Strand Gene LocusTag Fraction lambda1857_phage_12.5_MOI
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80,675 - ECD_00072 0.21 +0.1
80,676 + ECD_00072 0.21 -2.6
80,709 + ECD_00072 0.24 +0.5
80,722 + ECD_00072 0.25 -0.5
80,732 + ECD_00072 0.26 -1.8
80,796 + ECD_00072 0.31 -1.6
80,935 + ECD_00072 0.43 +1.2
80,936 - ECD_00072 0.43 -0.0
80,995 + ECD_00072 0.48 -2.0
80,996 - ECD_00072 0.48 +1.1
81,042 + ECD_00072 0.52 -1.3
81,042 + ECD_00072 0.52 +0.3
81,067 + ECD_00072 0.54 -0.7
81,070 + ECD_00072 0.55 -1.1
81,075 + ECD_00072 0.55 +0.7
81,075 + ECD_00072 0.55 +1.1
81,075 + ECD_00072 0.55 +0.5
81,085 - ECD_00072 0.56 -1.2
81,125 + ECD_00072 0.59 -3.8
81,125 + ECD_00072 0.59 +0.1
81,125 + ECD_00072 0.59 +0.1
81,125 + ECD_00072 0.59 -0.1
81,125 + ECD_00072 0.59 +1.2
81,126 - ECD_00072 0.59 -1.7
81,126 - ECD_00072 0.59 -2.4
81,126 - ECD_00072 0.59 +0.2
81,130 + ECD_00072 0.60 -1.8
81,130 + ECD_00072 0.60 -0.1
81,136 + ECD_00072 0.60 -3.2
81,245 + ECD_00072 0.69 -2.1
81,245 + ECD_00072 0.69 +0.9
81,246 - ECD_00072 0.70 +0.2
81,282 + ECD_00072 0.73 -1.3
81,309 - ECD_00072 0.75 +1.2
81,373 - ECD_00072 0.80 -2.2
81,405 + ECD_00072 0.83 +1.4
81,405 + ECD_00072 0.83 +1.4
81,406 - ECD_00072 0.83 -2.3
81,454 - ECD_00072 0.87 -2.5
81,458 - ECD_00072 0.88 -1.9
81,466 + ECD_00072 0.88 -1.3
81,467 - ECD_00072 0.88 +1.1
81,539 + -0.6
81,655 - +0.2
81,715 + ECD_00073 0.10 -0.6
81,919 + ECD_00073 0.44 +0.7
81,964 + ECD_00073 0.51 -1.8
81,965 - ECD_00073 0.51 -2.8
82,275 - -0.3
82,525 - ECD_00074 0.18 +0.4
82,696 + ECD_00074 0.30 -1.0
82,696 + ECD_00074 0.30 +1.0
82,696 + ECD_00074 0.30 +0.3
82,696 + ECD_00074 0.30 +1.8
82,696 + ECD_00074 0.30 -0.2
82,696 + ECD_00074 0.30 +0.1
82,697 - ECD_00074 0.31 -3.3
82,697 - ECD_00074 0.31 -0.0
82,760 + ECD_00074 0.35 +0.1
82,949 + ECD_00074 0.49 +0.2
82,949 + ECD_00074 0.49 +0.9
82,949 + ECD_00074 0.49 +1.0
82,949 + ECD_00074 0.49 -1.0
82,949 + ECD_00074 0.49 -1.6
82,950 - ECD_00074 0.49 -1.0
82,950 - ECD_00074 0.49 +0.1
82,950 - ECD_00074 0.49 -2.1
83,021 + ECD_00074 0.54 +0.6
83,037 + ECD_00074 0.55 -0.4
83,038 - ECD_00074 0.55 -2.3
83,038 - ECD_00074 0.55 -1.0
83,038 - ECD_00074 0.55 -2.4
83,135 + ECD_00074 0.62 -1.2
83,214 + ECD_00074 0.67 +1.3

Or see this region's nucleotide sequence