Experiment: lambda1857_phage_12.5_MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECD_00059 and ECD_00060 are separated by 116 nucleotides ECD_00060 and ECD_00061 are separated by 11 nucleotides ECD_00061 and ECD_00062 are separated by 164 nucleotides
ECD_00059: ECD_00059 - membrane-anchored DnaK co-chaperone, DNA-binding protein, at 61,560 to 62,375
_00059
ECD_00060: ECD_00060 - dual specificity 23S rRNA pseudouridine(746), tRNA pseudouridine(32) synthase, SAM-dependent, at 62,492 to 63,151
_00060
ECD_00061: ECD_00061 - RNA polymerase remodeling/recycling factor ATPase; RNA polymerase-associated, ATP-dependent RNA translocase, at 63,163 to 66,069
_00061
ECD_00062: ECD_00062 - DNA polymerase II, at 66,234 to 68,585
_00062
Position (kb) 63
64
65
66
67 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 62.180 kb on + strand, within ECD_00059 at 62.181 kb on - strand, within ECD_00059 at 62.237 kb on + strand, within ECD_00059 at 62.237 kb on + strand, within ECD_00059 at 62.238 kb on - strand, within ECD_00059 at 62.265 kb on - strand, within ECD_00059 at 62.340 kb on + strand at 62.340 kb on + strand at 62.341 kb on - strand at 62.369 kb on + strand at 62.388 kb on - strand at 62.513 kb on - strand at 62.521 kb on + strand at 62.521 kb on + strand at 62.521 kb on + strand at 62.522 kb on - strand at 62.525 kb on - strand at 62.568 kb on - strand, within ECD_00060 at 62.611 kb on - strand, within ECD_00060 at 62.650 kb on + strand, within ECD_00060 at 62.650 kb on + strand, within ECD_00060 at 62.651 kb on - strand, within ECD_00060 at 62.722 kb on - strand, within ECD_00060 at 62.736 kb on + strand, within ECD_00060 at 62.736 kb on + strand, within ECD_00060 at 62.937 kb on - strand, within ECD_00060 at 63.082 kb on + strand, within ECD_00060 at 63.082 kb on + strand, within ECD_00060 at 63.083 kb on - strand, within ECD_00060 at 63.083 kb on - strand, within ECD_00060 at 63.083 kb on - strand, within ECD_00060 at 63.083 kb on - strand, within ECD_00060 at 63.083 kb on - strand, within ECD_00060 at 63.083 kb on - strand, within ECD_00060 at 63.085 kb on + strand, within ECD_00060 at 63.085 kb on + strand, within ECD_00060 at 63.086 kb on - strand at 63.086 kb on - strand at 63.086 kb on - strand at 63.086 kb on - strand at 63.135 kb on + strand at 63.233 kb on - strand at 63.465 kb on + strand, within ECD_00061 at 63.474 kb on + strand, within ECD_00061 at 63.531 kb on + strand, within ECD_00061 at 63.543 kb on + strand, within ECD_00061 at 63.543 kb on + strand, within ECD_00061 at 63.543 kb on + strand, within ECD_00061 at 63.543 kb on + strand, within ECD_00061 at 63.543 kb on + strand, within ECD_00061 at 63.543 kb on + strand, within ECD_00061 at 63.543 kb on + strand, within ECD_00061 at 63.543 kb on + strand, within ECD_00061 at 63.544 kb on - strand, within ECD_00061 at 63.544 kb on - strand, within ECD_00061 at 63.544 kb on - strand, within ECD_00061 at 63.544 kb on - strand, within ECD_00061 at 63.544 kb on - strand, within ECD_00061 at 63.544 kb on - strand, within ECD_00061 at 63.544 kb on - strand, within ECD_00061 at 63.544 kb on - strand, within ECD_00061 at 63.544 kb on - strand, within ECD_00061 at 63.544 kb on - strand, within ECD_00061 at 63.544 kb on - strand, within ECD_00061 at 63.548 kb on - strand, within ECD_00061 at 63.611 kb on + strand, within ECD_00061 at 63.611 kb on + strand, within ECD_00061 at 63.611 kb on + strand, within ECD_00061 at 63.633 kb on + strand, within ECD_00061 at 63.633 kb on + strand, within ECD_00061 at 63.634 kb on - strand, within ECD_00061 at 63.742 kb on - strand, within ECD_00061 at 63.834 kb on + strand, within ECD_00061 at 63.834 kb on + strand, within ECD_00061 at 63.835 kb on - strand, within ECD_00061 at 63.835 kb on - strand, within ECD_00061 at 63.835 kb on - strand, within ECD_00061 at 64.112 kb on + strand, within ECD_00061 at 64.113 kb on - strand, within ECD_00061 at 64.113 kb on - strand, within ECD_00061 at 64.117 kb on - strand, within ECD_00061 at 64.130 kb on + strand, within ECD_00061 at 64.130 kb on + strand, within ECD_00061 at 64.130 kb on + strand, within ECD_00061 at 64.130 kb on + strand, within ECD_00061 at 64.130 kb on + strand, within ECD_00061 at 64.131 kb on - strand, within ECD_00061 at 64.131 kb on - strand, within ECD_00061 at 64.131 kb on - strand, within ECD_00061 at 64.131 kb on - strand, within ECD_00061 at 64.131 kb on - strand, within ECD_00061 at 64.132 kb on + strand, within ECD_00061 at 64.132 kb on + strand, within ECD_00061 at 64.132 kb on + strand, within ECD_00061 at 64.132 kb on + strand, within ECD_00061 at 64.132 kb on + strand, within ECD_00061 at 64.133 kb on - strand, within ECD_00061 at 64.133 kb on - strand, within ECD_00061 at 64.142 kb on + strand, within ECD_00061 at 64.143 kb on - strand, within ECD_00061 at 64.143 kb on - strand, within ECD_00061 at 64.147 kb on - strand, within ECD_00061 at 64.147 kb on - strand, within ECD_00061 at 64.235 kb on + strand, within ECD_00061 at 64.236 kb on - strand, within ECD_00061 at 64.291 kb on - strand, within ECD_00061 at 64.291 kb on - strand, within ECD_00061 at 64.303 kb on + strand, within ECD_00061 at 64.359 kb on + strand, within ECD_00061 at 64.393 kb on + strand, within ECD_00061 at 64.394 kb on - strand, within ECD_00061 at 64.394 kb on - strand, within ECD_00061 at 64.458 kb on + strand, within ECD_00061 at 64.458 kb on + strand, within ECD_00061 at 64.458 kb on + strand, within ECD_00061 at 64.458 kb on + strand, within ECD_00061 at 64.458 kb on + strand, within ECD_00061 at 64.458 kb on + strand, within ECD_00061 at 64.459 kb on - strand, within ECD_00061 at 64.505 kb on - strand, within ECD_00061 at 64.653 kb on + strand, within ECD_00061 at 64.654 kb on - strand, within ECD_00061 at 64.672 kb on - strand, within ECD_00061 at 64.698 kb on + strand, within ECD_00061 at 64.698 kb on + strand, within ECD_00061 at 64.699 kb on - strand, within ECD_00061 at 64.699 kb on - strand, within ECD_00061 at 64.725 kb on + strand, within ECD_00061 at 64.725 kb on + strand, within ECD_00061 at 64.762 kb on - strand, within ECD_00061 at 64.762 kb on - strand, within ECD_00061 at 64.762 kb on - strand, within ECD_00061 at 64.804 kb on - strand, within ECD_00061 at 65.184 kb on - strand, within ECD_00061 at 65.342 kb on + strand, within ECD_00061 at 65.342 kb on + strand, within ECD_00061 at 65.342 kb on + strand, within ECD_00061 at 65.342 kb on + strand, within ECD_00061 at 65.342 kb on + strand, within ECD_00061 at 65.343 kb on - strand, within ECD_00061 at 65.343 kb on - strand, within ECD_00061 at 65.343 kb on - strand, within ECD_00061 at 65.343 kb on - strand, within ECD_00061 at 65.343 kb on - strand, within ECD_00061 at 65.367 kb on - strand, within ECD_00061 at 65.438 kb on - strand, within ECD_00061 at 65.438 kb on - strand, within ECD_00061 at 65.438 kb on - strand, within ECD_00061 at 65.458 kb on + strand, within ECD_00061 at 65.458 kb on + strand, within ECD_00061 at 65.459 kb on - strand, within ECD_00061 at 65.459 kb on - strand, within ECD_00061 at 65.639 kb on + strand, within ECD_00061 at 65.639 kb on + strand, within ECD_00061 at 65.646 kb on + strand, within ECD_00061 at 65.670 kb on + strand, within ECD_00061 at 65.671 kb on - strand, within ECD_00061 at 65.761 kb on - strand, within ECD_00061 at 65.815 kb on - strand at 65.822 kb on + strand at 65.822 kb on + strand at 65.848 kb on + strand at 65.886 kb on + strand at 65.892 kb on + strand at 65.943 kb on - strand at 65.961 kb on + strand at 65.962 kb on - strand at 66.034 kb on - strand at 66.061 kb on - strand at 66.069 kb on + strand at 66.070 kb on - strand at 66.070 kb on - strand at 66.070 kb on - strand at 66.070 kb on - strand at 66.083 kb on + strand at 66.083 kb on + strand at 66.084 kb on - strand at 66.085 kb on + strand at 66.086 kb on - strand at 66.086 kb on - strand at 66.086 kb on - strand at 66.086 kb on - strand at 66.086 kb on - strand at 66.086 kb on - strand at 66.086 kb on - strand at 66.105 kb on - strand at 66.124 kb on + strand at 66.125 kb on - strand at 66.125 kb on - strand at 66.131 kb on + strand at 66.132 kb on - strand at 66.132 kb on - strand at 66.132 kb on - strand at 66.132 kb on - strand at 66.260 kb on + strand at 66.260 kb on + strand at 66.261 kb on - strand at 66.284 kb on + strand at 66.294 kb on + strand at 66.294 kb on + strand at 66.315 kb on + strand at 66.331 kb on + strand at 66.331 kb on + strand at 66.332 kb on - strand at 66.341 kb on - strand at 66.394 kb on - strand at 66.397 kb on + strand at 66.397 kb on + strand at 66.397 kb on + strand at 66.397 kb on + strand at 66.397 kb on + strand at 66.397 kb on + strand at 66.397 kb on + strand at 66.401 kb on + strand at 66.478 kb on - strand, within ECD_00062 at 66.494 kb on + strand, within ECD_00062 at 66.495 kb on - strand, within ECD_00062 at 66.535 kb on + strand, within ECD_00062 at 66.592 kb on + strand, within ECD_00062 at 66.593 kb on - strand, within ECD_00062 at 66.593 kb on - strand, within ECD_00062 at 66.593 kb on - strand, within ECD_00062 at 66.693 kb on - strand, within ECD_00062 at 66.816 kb on + strand, within ECD_00062 at 66.816 kb on + strand, within ECD_00062 at 66.958 kb on + strand, within ECD_00062 at 66.963 kb on - strand, within ECD_00062
Per-strain Table
Position Strand Gene LocusTag Fraction lambda1857_phage_12.5_MOI remove 62,180 + ECD_00059 0.76 -1.5 62,181 - ECD_00059 0.76 +1.0 62,237 + ECD_00059 0.83 -1.0 62,237 + ECD_00059 0.83 -0.9 62,238 - ECD_00059 0.83 +0.6 62,265 - ECD_00059 0.86 -1.7 62,340 + -0.2 62,340 + +1.1 62,341 - +1.1 62,369 + +0.3 62,388 - +0.1 62,513 - -0.1 62,521 + +0.7 62,521 + +0.7 62,521 + -1.4 62,522 - +1.9 62,525 - +1.6 62,568 - ECD_00060 0.12 +2.0 62,611 - ECD_00060 0.18 +0.4 62,650 + ECD_00060 0.24 -1.2 62,650 + ECD_00060 0.24 -0.8 62,651 - ECD_00060 0.24 -2.7 62,722 - ECD_00060 0.35 +0.1 62,736 + ECD_00060 0.37 -0.3 62,736 + ECD_00060 0.37 +0.3 62,937 - ECD_00060 0.67 +0.0 63,082 + ECD_00060 0.89 +0.3 63,082 + ECD_00060 0.89 -0.6 63,083 - ECD_00060 0.90 +0.8 63,083 - ECD_00060 0.90 -1.4 63,083 - ECD_00060 0.90 -0.1 63,083 - ECD_00060 0.90 -0.9 63,083 - ECD_00060 0.90 -3.2 63,083 - ECD_00060 0.90 -0.3 63,085 + ECD_00060 0.90 -0.1 63,085 + ECD_00060 0.90 +0.8 63,086 - +0.8 63,086 - -0.0 63,086 - +0.1 63,086 - -1.9 63,135 + +0.1 63,233 - +1.4 63,465 + ECD_00061 0.10 +1.7 63,474 + ECD_00061 0.11 +0.7 63,531 + ECD_00061 0.13 +2.6 63,543 + ECD_00061 0.13 +0.3 63,543 + ECD_00061 0.13 +0.7 63,543 + ECD_00061 0.13 +0.6 63,543 + ECD_00061 0.13 +1.3 63,543 + ECD_00061 0.13 +1.4 63,543 + ECD_00061 0.13 +1.1 63,543 + ECD_00061 0.13 +1.4 63,543 + ECD_00061 0.13 +2.0 63,544 - ECD_00061 0.13 +0.7 63,544 - ECD_00061 0.13 +1.3 63,544 - ECD_00061 0.13 +1.9 63,544 - ECD_00061 0.13 +0.6 63,544 - ECD_00061 0.13 +1.4 63,544 - ECD_00061 0.13 +1.0 63,544 - ECD_00061 0.13 +1.6 63,544 - ECD_00061 0.13 +0.1 63,544 - ECD_00061 0.13 +0.9 63,544 - ECD_00061 0.13 +1.3 63,544 - ECD_00061 0.13 +0.9 63,548 - ECD_00061 0.13 +1.6 63,611 + ECD_00061 0.15 +1.6 63,611 + ECD_00061 0.15 +1.0 63,611 + ECD_00061 0.15 +0.8 63,633 + ECD_00061 0.16 +0.7 63,633 + ECD_00061 0.16 +1.1 63,634 - ECD_00061 0.16 +0.1 63,742 - ECD_00061 0.20 -0.6 63,834 + ECD_00061 0.23 +0.4 63,834 + ECD_00061 0.23 +0.6 63,835 - ECD_00061 0.23 -0.4 63,835 - ECD_00061 0.23 +1.2 63,835 - ECD_00061 0.23 +1.6 64,112 + ECD_00061 0.33 +0.8 64,113 - ECD_00061 0.33 +1.6 64,113 - ECD_00061 0.33 +1.9 64,117 - ECD_00061 0.33 +2.5 64,130 + ECD_00061 0.33 +0.9 64,130 + ECD_00061 0.33 +1.1 64,130 + ECD_00061 0.33 +2.3 64,130 + ECD_00061 0.33 +0.6 64,130 + ECD_00061 0.33 +1.4 64,131 - ECD_00061 0.33 +1.6 64,131 - ECD_00061 0.33 +1.7 64,131 - ECD_00061 0.33 +1.0 64,131 - ECD_00061 0.33 +1.1 64,131 - ECD_00061 0.33 +1.7 64,132 + ECD_00061 0.33 +1.1 64,132 + ECD_00061 0.33 -1.1 64,132 + ECD_00061 0.33 -0.6 64,132 + ECD_00061 0.33 +1.4 64,132 + ECD_00061 0.33 +2.4 64,133 - ECD_00061 0.33 +0.9 64,133 - ECD_00061 0.33 +1.2 64,142 + ECD_00061 0.34 +0.6 64,143 - ECD_00061 0.34 +1.4 64,143 - ECD_00061 0.34 +2.2 64,147 - ECD_00061 0.34 +1.3 64,147 - ECD_00061 0.34 +0.3 64,235 + ECD_00061 0.37 -1.5 64,236 - ECD_00061 0.37 +1.4 64,291 - ECD_00061 0.39 +1.8 64,291 - ECD_00061 0.39 +2.0 64,303 + ECD_00061 0.39 +1.7 64,359 + ECD_00061 0.41 +1.1 64,393 + ECD_00061 0.42 +2.4 64,394 - ECD_00061 0.42 +1.2 64,394 - ECD_00061 0.42 +2.6 64,458 + ECD_00061 0.45 +2.8 64,458 + ECD_00061 0.45 +3.4 64,458 + ECD_00061 0.45 +1.9 64,458 + ECD_00061 0.45 -2.4 64,458 + ECD_00061 0.45 +1.7 64,458 + ECD_00061 0.45 -1.9 64,459 - ECD_00061 0.45 +1.4 64,505 - ECD_00061 0.46 +2.5 64,653 + ECD_00061 0.51 +1.5 64,654 - ECD_00061 0.51 +1.9 64,672 - ECD_00061 0.52 -0.1 64,698 + ECD_00061 0.53 +1.2 64,698 + ECD_00061 0.53 -0.9 64,699 - ECD_00061 0.53 -0.8 64,699 - ECD_00061 0.53 +0.2 64,725 + ECD_00061 0.54 +0.8 64,725 + ECD_00061 0.54 -2.6 64,762 - ECD_00061 0.55 +0.9 64,762 - ECD_00061 0.55 -0.1 64,762 - ECD_00061 0.55 -0.3 64,804 - ECD_00061 0.56 +0.5 65,184 - ECD_00061 0.70 +1.6 65,342 + ECD_00061 0.75 +0.2 65,342 + ECD_00061 0.75 +1.2 65,342 + ECD_00061 0.75 +0.9 65,342 + ECD_00061 0.75 +1.6 65,342 + ECD_00061 0.75 +0.8 65,343 - ECD_00061 0.75 -1.7 65,343 - ECD_00061 0.75 +0.6 65,343 - ECD_00061 0.75 +1.6 65,343 - ECD_00061 0.75 +0.1 65,343 - ECD_00061 0.75 -0.4 65,367 - ECD_00061 0.76 +0.6 65,438 - ECD_00061 0.78 +0.3 65,438 - ECD_00061 0.78 +0.8 65,438 - ECD_00061 0.78 +1.1 65,458 + ECD_00061 0.79 +2.8 65,458 + ECD_00061 0.79 +0.4 65,459 - ECD_00061 0.79 +0.6 65,459 - ECD_00061 0.79 +0.9 65,639 + ECD_00061 0.85 -0.0 65,639 + ECD_00061 0.85 -2.2 65,646 + ECD_00061 0.85 +2.6 65,670 + ECD_00061 0.86 +1.6 65,671 - ECD_00061 0.86 +1.6 65,761 - ECD_00061 0.89 -0.6 65,815 - +0.2 65,822 + +1.8 65,822 + +1.5 65,848 + +1.6 65,886 + +0.4 65,892 + +0.2 65,943 - +1.4 65,961 + +0.4 65,962 - +1.1 66,034 - +0.7 66,061 - -0.1 66,069 + +1.5 66,070 - +2.0 66,070 - -0.7 66,070 - +1.6 66,070 - +0.8 66,083 + +1.0 66,083 + +2.0 66,084 - +0.5 66,085 + +1.5 66,086 - -1.0 66,086 - +1.3 66,086 - -0.4 66,086 - -0.7 66,086 - -1.2 66,086 - +0.8 66,086 - -1.7 66,105 - -1.1 66,124 + +1.3 66,125 - +1.3 66,125 - +0.6 66,131 + -0.6 66,132 - +0.4 66,132 - +1.9 66,132 - -0.2 66,132 - +0.1 66,260 + +0.7 66,260 + -2.0 66,261 - +0.5 66,284 + +2.5 66,294 + -0.6 66,294 + -0.2 66,315 + +0.2 66,331 + -0.8 66,331 + -0.9 66,332 - -0.1 66,341 - -2.0 66,394 - -1.9 66,397 + +0.5 66,397 + +0.3 66,397 + -0.0 66,397 + -0.6 66,397 + +0.7 66,397 + +1.0 66,397 + -0.1 66,401 + -3.0 66,478 - ECD_00062 0.10 -1.8 66,494 + ECD_00062 0.11 -2.0 66,495 - ECD_00062 0.11 +1.3 66,535 + ECD_00062 0.13 -2.2 66,592 + ECD_00062 0.15 +0.5 66,593 - ECD_00062 0.15 -0.5 66,593 - ECD_00062 0.15 -1.1 66,593 - ECD_00062 0.15 +1.1 66,693 - ECD_00062 0.20 +0.7 66,816 + ECD_00062 0.25 -0.3 66,816 + ECD_00062 0.25 -0.9 66,958 + ECD_00062 0.31 +1.0 66,963 - ECD_00062 0.31 +0.1
Or see this region's nucleotide sequence