Strain Fitness in Escherichia coli BL21 around ECD_00036

Experiment: lambda1857_phage_12.5_MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00035 and ECD_00036 are separated by 455 nucleotidesECD_00036 and ECD_00037 are separated by 17 nucleotides ECD_00035: ECD_00035 - dihydrodipicolinate reductase, at 32,446 to 33,267 _00035 ECD_00036: ECD_00036 - carbamoyl phosphate synthetase small subunit, glutamine amidotransferase, at 33,723 to 34,871 _00036 ECD_00037: ECD_00037 - carbamoyl-phosphate synthase large subunit, at 34,889 to 38,110 _00037 Position (kb) 33 34 35Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 33.864 kb on + strand, within ECD_00036at 33.864 kb on + strand, within ECD_00036at 33.881 kb on + strand, within ECD_00036at 33.887 kb on + strand, within ECD_00036at 33.887 kb on + strand, within ECD_00036at 33.887 kb on + strand, within ECD_00036at 33.887 kb on + strand, within ECD_00036at 33.888 kb on - strand, within ECD_00036at 33.888 kb on - strand, within ECD_00036at 33.891 kb on + strand, within ECD_00036at 33.891 kb on + strand, within ECD_00036at 33.892 kb on - strand, within ECD_00036at 33.946 kb on + strand, within ECD_00036at 34.004 kb on + strand, within ECD_00036at 34.038 kb on - strand, within ECD_00036at 34.224 kb on + strand, within ECD_00036at 34.226 kb on + strand, within ECD_00036at 34.685 kb on - strand, within ECD_00036at 34.710 kb on - strand, within ECD_00036at 34.710 kb on - strand, within ECD_00036at 34.857 kb on - strandat 34.869 kb on + strandat 34.879 kb on + strandat 34.900 kb on + strandat 34.901 kb on - strandat 34.906 kb on + strandat 34.909 kb on - strandat 34.915 kb on + strandat 34.970 kb on + strandat 34.990 kb on + strandat 34.990 kb on + strandat 34.990 kb on + strandat 34.990 kb on + strandat 34.990 kb on + strandat 34.991 kb on - strandat 35.081 kb on + strandat 35.082 kb on - strandat 35.082 kb on - strandat 35.106 kb on + strandat 35.107 kb on - strandat 35.230 kb on + strand, within ECD_00037at 35.404 kb on + strand, within ECD_00037at 35.436 kb on - strand, within ECD_00037at 35.669 kb on + strand, within ECD_00037

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Per-strain Table

Position Strand Gene LocusTag Fraction lambda1857_phage_12.5_MOI
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33,864 + ECD_00036 0.12 -0.6
33,864 + ECD_00036 0.12 -2.1
33,881 + ECD_00036 0.14 -2.5
33,887 + ECD_00036 0.14 -1.9
33,887 + ECD_00036 0.14 -1.1
33,887 + ECD_00036 0.14 +0.3
33,887 + ECD_00036 0.14 -0.3
33,888 - ECD_00036 0.14 -3.1
33,888 - ECD_00036 0.14 -1.8
33,891 + ECD_00036 0.15 -1.3
33,891 + ECD_00036 0.15 -2.0
33,892 - ECD_00036 0.15 +1.2
33,946 + ECD_00036 0.19 -1.9
34,004 + ECD_00036 0.24 -1.6
34,038 - ECD_00036 0.27 -0.5
34,224 + ECD_00036 0.44 -4.2
34,226 + ECD_00036 0.44 -3.3
34,685 - ECD_00036 0.84 -2.0
34,710 - ECD_00036 0.86 -1.3
34,710 - ECD_00036 0.86 -3.3
34,857 - +0.7
34,869 + +0.9
34,879 + -1.2
34,900 + -1.5
34,901 - -1.3
34,906 + +0.2
34,909 - -0.7
34,915 + -0.0
34,970 + -2.1
34,990 + -1.3
34,990 + -2.1
34,990 + -2.9
34,990 + +0.4
34,990 + -1.8
34,991 - -0.2
35,081 + -0.9
35,082 - -0.9
35,082 - -1.9
35,106 + +0.0
35,107 - +1.1
35,230 + ECD_00037 0.11 -1.4
35,404 + ECD_00037 0.16 -0.5
35,436 - ECD_00037 0.17 +0.7
35,669 + ECD_00037 0.24 -0.6

Or see this region's nucleotide sequence