Strain Fitness in Escherichia coli BL21 around ECD_03451

Experiment: P2_phage_0.5625_MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_03449 and ECD_03450 are separated by 41 nucleotidesECD_03450 and ECD_03451 are separated by 11 nucleotides ECD_03449: ECD_03449 - putative immunity protein for polymorphic toxin RhsA; HEAT-domain protein; lethality reduction protein, at 3,629,466 to 3,630,308 _03449 ECD_03450: ECD_03450 - putative Rhs-family protein, at 3,630,350 to 3,631,294 _03450 ECD_03451: ECD_03451 - TPR-like repeat protein, at 3,631,306 to 3,631,767 _03451 Position (kb) 3631 3632Strain fitness (log2 ratio) -3 -2 -1 0 1at 3630.321 kb on - strandat 3630.651 kb on - strand, within ECD_03450at 3630.674 kb on + strand, within ECD_03450at 3630.692 kb on + strand, within ECD_03450at 3630.692 kb on + strand, within ECD_03450at 3630.789 kb on + strand, within ECD_03450at 3630.789 kb on + strand, within ECD_03450at 3630.911 kb on - strand, within ECD_03450at 3631.058 kb on - strand, within ECD_03450at 3631.300 kb on - strandat 3631.502 kb on - strand, within ECD_03451at 3631.689 kb on + strand, within ECD_03451at 3631.798 kb on + strandat 3631.801 kb on + strandat 3631.802 kb on - strandat 3631.826 kb on - strandat 3631.827 kb on + strandat 3631.827 kb on + strandat 3631.867 kb on - strandat 3632.632 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction P2_phage_0.5625_MOI
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3,630,321 - -0.6
3,630,651 - ECD_03450 0.32 +0.6
3,630,674 + ECD_03450 0.34 -3.7
3,630,692 + ECD_03450 0.36 -2.4
3,630,692 + ECD_03450 0.36 -2.0
3,630,789 + ECD_03450 0.46 -0.8
3,630,789 + ECD_03450 0.46 -0.2
3,630,911 - ECD_03450 0.59 +1.2
3,631,058 - ECD_03450 0.75 -3.2
3,631,300 - -1.5
3,631,502 - ECD_03451 0.42 -3.4
3,631,689 + ECD_03451 0.83 -1.9
3,631,798 + -0.6
3,631,801 + -1.5
3,631,802 - -1.1
3,631,826 - +0.6
3,631,827 + -0.5
3,631,827 + -2.7
3,631,867 - -2.4
3,632,632 - -2.8

Or see this region's nucleotide sequence