Experiment: P2_phage_0.5625_MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECD_03141 and ECD_03142 overlap by 4 nucleotides ECD_03142 and ECD_03143 are separated by 55 nucleotides ECD_03143 and ECD_03144 are separated by 10 nucleotides ECD_03144 and ECD_03145 are separated by 106 nucleotides ECD_03145 and ECD_03146 are separated by 40 nucleotides
ECD_03141: ECD_03141 - mechanosensitive channel protein, high conductance, at 3,299,194 to 3,299,604
_03141
ECD_03142: ECD_03142 - alternate ribosome-rescue factor A, at 3,299,601 to 3,299,819
_03142
ECD_03143: ECD_03143 - zntA gene transcriptional activator, at 3,299,875 to 3,300,300
_03143
ECD_03144: ECD_03144 - DUF1992 family protein, at 3,300,311 to 3,300,679
_03144
ECD_03145: ECD_03145 - 50S ribosomal subunit protein L17, at 3,300,786 to 3,301,169
_03145
ECD_03146: ECD_03146 - RNA polymerase, alpha subunit, at 3,301,210 to 3,302,199
_03146
Position (kb)
3300
3301 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3299.334 kb on - strand, within ECD_03141 at 3299.481 kb on + strand, within ECD_03141 at 3299.486 kb on + strand, within ECD_03141 at 3299.542 kb on - strand, within ECD_03141 at 3299.552 kb on + strand, within ECD_03141 at 3299.552 kb on + strand, within ECD_03141 at 3299.555 kb on + strand, within ECD_03141 at 3299.704 kb on + strand, within ECD_03142 at 3299.705 kb on - strand, within ECD_03142 at 3299.764 kb on - strand, within ECD_03142 at 3299.773 kb on - strand, within ECD_03142 at 3299.781 kb on - strand, within ECD_03142 at 3299.788 kb on - strand, within ECD_03142 at 3299.799 kb on - strand at 3299.837 kb on - strand at 3299.943 kb on + strand, within ECD_03143 at 3299.994 kb on + strand, within ECD_03143 at 3299.995 kb on - strand, within ECD_03143 at 3300.070 kb on - strand, within ECD_03143 at 3300.070 kb on - strand, within ECD_03143 at 3300.181 kb on - strand, within ECD_03143 at 3300.263 kb on - strand at 3300.300 kb on + strand at 3300.300 kb on + strand at 3300.300 kb on + strand at 3300.300 kb on + strand at 3300.301 kb on - strand at 3300.301 kb on - strand at 3300.301 kb on - strand at 3300.301 kb on - strand at 3300.301 kb on - strand at 3300.301 kb on - strand at 3300.301 kb on - strand at 3300.301 kb on - strand at 3300.301 kb on - strand at 3300.301 kb on - strand at 3300.301 kb on - strand at 3300.305 kb on - strand at 3300.305 kb on - strand at 3300.313 kb on - strand at 3300.513 kb on - strand, within ECD_03144 at 3300.645 kb on - strand at 3300.645 kb on - strand at 3300.645 kb on - strand at 3300.645 kb on - strand at 3300.647 kb on - strand at 3300.672 kb on - strand at 3300.672 kb on - strand at 3300.684 kb on - strand at 3300.709 kb on - strand at 3300.720 kb on - strand at 3300.724 kb on - strand at 3300.724 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P2_phage_0.5625_MOI remove 3,299,334 - ECD_03141 0.34 -1.5 3,299,481 + ECD_03141 0.70 -0.7 3,299,486 + ECD_03141 0.71 +1.7 3,299,542 - ECD_03141 0.85 -0.3 3,299,552 + ECD_03141 0.87 +1.4 3,299,552 + ECD_03141 0.87 -0.1 3,299,555 + ECD_03141 0.88 +0.8 3,299,704 + ECD_03142 0.47 -0.2 3,299,705 - ECD_03142 0.47 -1.5 3,299,764 - ECD_03142 0.74 -0.5 3,299,773 - ECD_03142 0.79 -2.5 3,299,781 - ECD_03142 0.82 -1.4 3,299,788 - ECD_03142 0.85 -2.6 3,299,799 - -0.7 3,299,837 - +1.0 3,299,943 + ECD_03143 0.16 -1.4 3,299,994 + ECD_03143 0.28 -2.8 3,299,995 - ECD_03143 0.28 +0.1 3,300,070 - ECD_03143 0.46 +1.3 3,300,070 - ECD_03143 0.46 +0.9 3,300,181 - ECD_03143 0.72 -1.5 3,300,263 - -2.0 3,300,300 + +0.1 3,300,300 + +0.3 3,300,300 + -1.5 3,300,300 + +0.5 3,300,301 - +1.1 3,300,301 - -3.2 3,300,301 - +1.3 3,300,301 - +1.7 3,300,301 - -0.7 3,300,301 - -0.9 3,300,301 - -0.9 3,300,301 - -2.0 3,300,301 - -1.4 3,300,301 - -1.1 3,300,301 - -0.9 3,300,305 - -1.9 3,300,305 - -2.0 3,300,313 - -1.1 3,300,513 - ECD_03144 0.55 -2.4 3,300,645 - -1.9 3,300,645 - -3.6 3,300,645 - -0.6 3,300,645 - -2.0 3,300,647 - -1.3 3,300,672 - -2.0 3,300,672 - -2.7 3,300,684 - -1.2 3,300,709 - -0.9 3,300,720 - -3.3 3,300,724 - -0.3 3,300,724 - -2.0
Or see this region's nucleotide sequence