Strain Fitness in Escherichia coli BL21 around ECD_02459

Experiment: P2_phage_0.5625_MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_02457 and ECD_02458 overlap by 1 nucleotidesECD_02458 and ECD_02459 are separated by 11 nucleotidesECD_02459 and ECD_02460 are separated by 11 nucleotidesECD_02460 and ECD_02461 overlap by 4 nucleotides ECD_02457: ECD_02457 - holo-[acyl-carrier-protein] synthase 1, at 2,567,217 to 2,567,597 _02457 ECD_02458: ECD_02458 - pyridoxine 5'-phosphate synthase, at 2,567,597 to 2,568,328 _02458 ECD_02459: ECD_02459 - gap repair protein, at 2,568,340 to 2,569,068 _02459 ECD_02460: ECD_02460 - membrane-associated, 16S rRNA-binding GTPase, at 2,569,080 to 2,569,985 _02460 ECD_02461: ECD_02461 - RNase III, at 2,569,982 to 2,570,662 _02461 Position (kb) 2568 2569 2570Strain fitness (log2 ratio) -2 -1 0 1at 2567.740 kb on - strand, within ECD_02458at 2568.348 kb on - strandat 2568.579 kb on - strand, within ECD_02459at 2568.798 kb on - strand, within ECD_02459at 2568.827 kb on - strand, within ECD_02459at 2568.937 kb on - strand, within ECD_02459at 2569.035 kb on + strandat 2569.082 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction P2_phage_0.5625_MOI
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2,567,740 - ECD_02458 0.20 -0.2
2,568,348 - +0.3
2,568,579 - ECD_02459 0.33 -1.3
2,568,798 - ECD_02459 0.63 -2.5
2,568,827 - ECD_02459 0.67 -2.4
2,568,937 - ECD_02459 0.82 +0.2
2,569,035 + +0.2
2,569,082 - -0.2

Or see this region's nucleotide sequence