Strain Fitness in Escherichia coli BL21 around ECD_02443

Experiment: P2_phage_0.5625_MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_02442 and ECD_02443 are separated by 197 nucleotidesECD_02443 and ECD_02444 are separated by 327 nucleotides ECD_02442: ECD_02442 - putative DNA-binding transcriptional regulator, at 2,548,668 to 2,549,861 _02442 ECD_02443: ECD_02443 - serine hydroxymethyltransferase, at 2,550,059 to 2,551,312 _02443 ECD_02444: ECD_02444 - fused nitric oxide dioxygenase/dihydropteridine reductase 2, at 2,551,640 to 2,552,830 _02444 Position (kb) 2550 2551 2552Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 2549.066 kb on + strand, within ECD_02442at 2549.183 kb on - strand, within ECD_02442at 2549.187 kb on + strand, within ECD_02442at 2549.188 kb on - strand, within ECD_02442at 2549.364 kb on + strand, within ECD_02442at 2549.670 kb on + strand, within ECD_02442at 2549.708 kb on + strand, within ECD_02442at 2549.708 kb on + strand, within ECD_02442at 2549.708 kb on + strand, within ECD_02442at 2549.709 kb on - strand, within ECD_02442at 2549.711 kb on + strand, within ECD_02442at 2549.794 kb on + strandat 2549.794 kb on + strandat 2549.826 kb on + strandat 2549.832 kb on + strandat 2549.833 kb on - strandat 2549.964 kb on + strandat 2550.067 kb on - strandat 2550.417 kb on - strand, within ECD_02443at 2550.644 kb on - strand, within ECD_02443at 2550.713 kb on - strand, within ECD_02443at 2550.727 kb on - strand, within ECD_02443at 2550.895 kb on - strand, within ECD_02443at 2550.971 kb on - strand, within ECD_02443at 2551.123 kb on - strand, within ECD_02443at 2551.150 kb on - strand, within ECD_02443at 2551.259 kb on - strandat 2551.568 kb on + strandat 2551.725 kb on - strandat 2551.732 kb on + strandat 2551.732 kb on + strandat 2551.820 kb on - strand, within ECD_02444at 2551.922 kb on + strand, within ECD_02444at 2552.009 kb on + strand, within ECD_02444at 2552.016 kb on + strand, within ECD_02444at 2552.016 kb on + strand, within ECD_02444at 2552.016 kb on + strand, within ECD_02444at 2552.028 kb on + strand, within ECD_02444at 2552.028 kb on + strand, within ECD_02444at 2552.033 kb on - strand, within ECD_02444at 2552.054 kb on + strand, within ECD_02444at 2552.293 kb on + strand, within ECD_02444

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Per-strain Table

Position Strand Gene LocusTag Fraction P2_phage_0.5625_MOI
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2,549,066 + ECD_02442 0.33 -0.6
2,549,183 - ECD_02442 0.43 -1.0
2,549,187 + ECD_02442 0.43 +2.1
2,549,188 - ECD_02442 0.44 -1.3
2,549,364 + ECD_02442 0.58 +0.1
2,549,670 + ECD_02442 0.84 -1.2
2,549,708 + ECD_02442 0.87 -1.3
2,549,708 + ECD_02442 0.87 -0.7
2,549,708 + ECD_02442 0.87 -2.1
2,549,709 - ECD_02442 0.87 +2.1
2,549,711 + ECD_02442 0.87 -0.2
2,549,794 + +3.5
2,549,794 + +0.1
2,549,826 + -2.2
2,549,832 + -1.1
2,549,833 - -0.8
2,549,964 + +1.6
2,550,067 - +2.7
2,550,417 - ECD_02443 0.29 -0.6
2,550,644 - ECD_02443 0.47 -1.0
2,550,713 - ECD_02443 0.52 -1.0
2,550,727 - ECD_02443 0.53 -2.0
2,550,895 - ECD_02443 0.67 -1.3
2,550,971 - ECD_02443 0.73 -2.6
2,551,123 - ECD_02443 0.85 -2.3
2,551,150 - ECD_02443 0.87 -3.4
2,551,259 - -2.3
2,551,568 + +0.8
2,551,725 - -2.3
2,551,732 + +0.3
2,551,732 + -0.2
2,551,820 - ECD_02444 0.15 +0.9
2,551,922 + ECD_02444 0.24 -1.1
2,552,009 + ECD_02444 0.31 -1.5
2,552,016 + ECD_02444 0.32 +0.8
2,552,016 + ECD_02444 0.32 +0.2
2,552,016 + ECD_02444 0.32 -0.7
2,552,028 + ECD_02444 0.33 +3.2
2,552,028 + ECD_02444 0.33 -2.0
2,552,033 - ECD_02444 0.33 -1.8
2,552,054 + ECD_02444 0.35 -1.2
2,552,293 + ECD_02444 0.55 -1.3

Or see this region's nucleotide sequence