Strain Fitness in Escherichia coli BL21 around ECD_02432

Experiment: P2_phage_0.5625_MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_02430 and ECD_02431 overlap by 4 nucleotidesECD_02431 and ECD_02432 overlap by 1 nucleotidesECD_02432 and ECD_02433 overlap by 4 nucleotidesECD_02433 and ECD_02434 are separated by 9 nucleotides ECD_02430: ECD_02430 - 3-phenylpropionate dioxygenase, large (alpha) subunit, at 2,534,837 to 2,536,198 _02430 ECD_02431: ECD_02431 - 3-phenylpropionate dioxygenase, small (beta) subunit, at 2,536,195 to 2,536,713 _02431 ECD_02432: ECD_02432 - 3-phenylpropionate dioxygenase, ferredoxin subunit, at 2,536,713 to 2,537,033 _02432 ECD_02433: ECD_02433 - 2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase, at 2,537,030 to 2,537,842 _02433 ECD_02434: ECD_02434 - phenylpropionate dioxygenase, ferredoxin reductase subunit, at 2,537,852 to 2,539,054 _02434 Position (kb) 2536 2537 2538Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2535.721 kb on - strand, within ECD_02430at 2535.755 kb on - strand, within ECD_02430at 2535.760 kb on + strand, within ECD_02430at 2535.884 kb on - strand, within ECD_02430at 2536.072 kb on + strandat 2536.073 kb on - strandat 2536.073 kb on - strandat 2536.073 kb on - strandat 2536.073 kb on - strandat 2536.073 kb on - strandat 2536.220 kb on + strandat 2536.220 kb on + strandat 2536.221 kb on - strandat 2536.488 kb on - strand, within ECD_02431at 2536.553 kb on + strand, within ECD_02431at 2536.592 kb on - strand, within ECD_02431at 2536.659 kb on + strand, within ECD_02431at 2536.703 kb on + strandat 2536.704 kb on - strandat 2536.725 kb on + strandat 2536.775 kb on + strand, within ECD_02432at 2536.847 kb on + strand, within ECD_02432at 2537.189 kb on - strand, within ECD_02433at 2537.324 kb on - strand, within ECD_02433at 2537.327 kb on - strand, within ECD_02433at 2537.327 kb on - strand, within ECD_02433at 2537.379 kb on + strand, within ECD_02433at 2537.380 kb on - strand, within ECD_02433at 2537.380 kb on - strand, within ECD_02433at 2537.495 kb on + strand, within ECD_02433at 2537.544 kb on - strand, within ECD_02433at 2537.590 kb on + strand, within ECD_02433at 2537.727 kb on - strand, within ECD_02433at 2537.872 kb on + strandat 2537.975 kb on + strand, within ECD_02434at 2538.027 kb on + strand, within ECD_02434

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Per-strain Table

Position Strand Gene LocusTag Fraction P2_phage_0.5625_MOI
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2,535,721 - ECD_02430 0.65 -2.4
2,535,755 - ECD_02430 0.67 +1.5
2,535,760 + ECD_02430 0.68 -1.8
2,535,884 - ECD_02430 0.77 +1.7
2,536,072 + -0.6
2,536,073 - -0.6
2,536,073 - -1.0
2,536,073 - +0.3
2,536,073 - +0.9
2,536,073 - +0.2
2,536,220 + +1.0
2,536,220 + +0.7
2,536,221 - +0.7
2,536,488 - ECD_02431 0.56 -0.6
2,536,553 + ECD_02431 0.69 -0.4
2,536,592 - ECD_02431 0.76 -1.4
2,536,659 + ECD_02431 0.89 -0.8
2,536,703 + +0.7
2,536,704 - +0.9
2,536,725 + -2.0
2,536,775 + ECD_02432 0.19 -1.1
2,536,847 + ECD_02432 0.42 -1.5
2,537,189 - ECD_02433 0.20 -1.9
2,537,324 - ECD_02433 0.36 -0.3
2,537,327 - ECD_02433 0.37 -2.0
2,537,327 - ECD_02433 0.37 -1.6
2,537,379 + ECD_02433 0.43 -0.1
2,537,380 - ECD_02433 0.43 -2.7
2,537,380 - ECD_02433 0.43 +2.4
2,537,495 + ECD_02433 0.57 -1.6
2,537,544 - ECD_02433 0.63 -2.8
2,537,590 + ECD_02433 0.69 +1.3
2,537,727 - ECD_02433 0.86 -0.2
2,537,872 + -1.3
2,537,975 + ECD_02434 0.10 -0.3
2,538,027 + ECD_02434 0.15 +1.9

Or see this region's nucleotide sequence