Strain Fitness in Escherichia coli BL21 around ECD_02254

Experiment: P2_phage_0.5625_MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_02252 and ECD_02253 overlap by 1 nucleotidesECD_02253 and ECD_02254 are separated by 3 nucleotidesECD_02254 and ECD_02255 are separated by 34 nucleotides ECD_02252: ECD_02252 - TauE/TSUP family inner membrane protein, at 2,332,004 to 2,332,813 _02252 ECD_02253: ECD_02253 - murein DD-endopeptidase, at 2,332,813 to 2,333,637 _02253 ECD_02254: ECD_02254 - chorismate synthase, at 2,333,641 to 2,334,726 _02254 ECD_02255: ECD_02255 - N5-glutamine methyltransferase, at 2,334,761 to 2,335,693 _02255 Position (kb) 2333 2334 2335Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2332.789 kb on + strandat 2332.801 kb on + strandat 2332.814 kb on + strandat 2332.997 kb on - strand, within ECD_02253at 2332.997 kb on - strand, within ECD_02253at 2332.997 kb on - strand, within ECD_02253at 2333.002 kb on + strand, within ECD_02253at 2333.003 kb on - strand, within ECD_02253at 2333.003 kb on - strand, within ECD_02253at 2333.435 kb on + strand, within ECD_02253at 2333.466 kb on - strand, within ECD_02253at 2333.506 kb on - strand, within ECD_02253at 2333.515 kb on - strand, within ECD_02253at 2333.515 kb on - strand, within ECD_02253at 2333.818 kb on - strand, within ECD_02254at 2334.401 kb on + strand, within ECD_02254at 2334.402 kb on - strand, within ECD_02254at 2334.433 kb on - strand, within ECD_02254at 2334.821 kb on - strandat 2334.887 kb on - strand, within ECD_02255at 2334.894 kb on - strand, within ECD_02255at 2334.894 kb on - strand, within ECD_02255at 2335.027 kb on - strand, within ECD_02255at 2335.049 kb on - strand, within ECD_02255at 2335.279 kb on + strand, within ECD_02255at 2335.419 kb on + strand, within ECD_02255at 2335.420 kb on - strand, within ECD_02255at 2335.557 kb on - strand, within ECD_02255at 2335.665 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction P2_phage_0.5625_MOI
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2,332,789 + -1.9
2,332,801 + -0.3
2,332,814 + +1.4
2,332,997 - ECD_02253 0.22 -0.7
2,332,997 - ECD_02253 0.22 +2.0
2,332,997 - ECD_02253 0.22 -2.7
2,333,002 + ECD_02253 0.23 -1.4
2,333,003 - ECD_02253 0.23 -1.7
2,333,003 - ECD_02253 0.23 +1.6
2,333,435 + ECD_02253 0.75 -1.0
2,333,466 - ECD_02253 0.79 +2.1
2,333,506 - ECD_02253 0.84 -0.9
2,333,515 - ECD_02253 0.85 +1.5
2,333,515 - ECD_02253 0.85 +1.2
2,333,818 - ECD_02254 0.16 -1.1
2,334,401 + ECD_02254 0.70 -0.1
2,334,402 - ECD_02254 0.70 -1.5
2,334,433 - ECD_02254 0.73 -0.8
2,334,821 - +1.3
2,334,887 - ECD_02255 0.14 +0.9
2,334,894 - ECD_02255 0.14 -2.3
2,334,894 - ECD_02255 0.14 +3.0
2,335,027 - ECD_02255 0.29 +1.7
2,335,049 - ECD_02255 0.31 +1.5
2,335,279 + ECD_02255 0.56 +0.7
2,335,419 + ECD_02255 0.71 +0.2
2,335,420 - ECD_02255 0.71 +0.2
2,335,557 - ECD_02255 0.85 -1.8
2,335,665 - -1.3

Or see this region's nucleotide sequence