Experiment: P2_phage_0.5625_MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECD_01956 and ECD_01957 overlap by 4 nucleotides ECD_01957 and ECD_01958 are separated by 2 nucleotides ECD_01958 and ECD_01959 are separated by 2 nucleotides
ECD_01956: ECD_01956 - putative glycosyl transferase, at 2,023,529 to 2,024,752
_01956
ECD_01957: ECD_01957 - GDP-mannose mannosyl hydrolase, at 2,024,749 to 2,025,228
_01957
ECD_01958: ECD_01958 - bifunctional GDP-fucose synthetase: GDP-4-dehydro-6-deoxy-D-mannose epimerase/ GDP-4-dehydro-6-L-deoxygalactose reductase, at 2,025,231 to 2,026,196
_01958
ECD_01959: ECD_01959 - GDP-D-mannose dehydratase, NAD(P)-binding, at 2,026,199 to 2,027,320
_01959
Position (kb)
2025
2026
2027 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2024.535 kb on + strand, within ECD_01956 at 2024.596 kb on - strand, within ECD_01956 at 2024.626 kb on - strand, within ECD_01956 at 2024.925 kb on - strand, within ECD_01957 at 2024.963 kb on + strand, within ECD_01957 at 2025.233 kb on - strand at 2025.271 kb on + strand at 2025.360 kb on - strand, within ECD_01958 at 2025.748 kb on + strand, within ECD_01958 at 2025.869 kb on + strand, within ECD_01958 at 2025.870 kb on - strand, within ECD_01958 at 2026.098 kb on + strand, within ECD_01958 at 2026.099 kb on - strand, within ECD_01958 at 2026.228 kb on - strand at 2026.544 kb on + strand, within ECD_01959 at 2026.620 kb on - strand, within ECD_01959 at 2026.620 kb on - strand, within ECD_01959 at 2026.833 kb on + strand, within ECD_01959 at 2027.017 kb on + strand, within ECD_01959 at 2027.120 kb on - strand, within ECD_01959 at 2027.120 kb on - strand, within ECD_01959
Per-strain Table
Position Strand Gene LocusTag Fraction P2_phage_0.5625_MOI remove 2,024,535 + ECD_01956 0.82 -2.1 2,024,596 - ECD_01956 0.87 +0.7 2,024,626 - ECD_01956 0.90 -0.9 2,024,925 - ECD_01957 0.37 -1.2 2,024,963 + ECD_01957 0.45 +1.9 2,025,233 - +2.4 2,025,271 + -0.1 2,025,360 - ECD_01958 0.13 -1.5 2,025,748 + ECD_01958 0.54 +0.1 2,025,869 + ECD_01958 0.66 -1.4 2,025,870 - ECD_01958 0.66 -0.5 2,026,098 + ECD_01958 0.90 -0.4 2,026,099 - ECD_01958 0.90 +0.3 2,026,228 - -1.3 2,026,544 + ECD_01959 0.31 -0.9 2,026,620 - ECD_01959 0.38 -1.6 2,026,620 - ECD_01959 0.38 -1.0 2,026,833 + ECD_01959 0.57 +2.5 2,027,017 + ECD_01959 0.73 -0.7 2,027,120 - ECD_01959 0.82 -0.7 2,027,120 - ECD_01959 0.82 -0.2
Or see this region's nucleotide sequence