Strain Fitness in Escherichia coli BL21 around ECD_01952

Experiment: P2_phage_0.5625_MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01951 and ECD_01952 are separated by 161 nucleotidesECD_01952 and ECD_01953 are separated by 1 nucleotides ECD_01951: ECD_01951 - colanic acid biosynthesis protein, at 2,016,156 to 2,017,436 _01951 ECD_01952: ECD_01952 - putative colanic acid exporter, at 2,017,598 to 2,019,076 _01952 ECD_01953: ECD_01953 - colanic biosynthesis UDP-glucose lipid carrier transferase, at 2,019,078 to 2,020,472 _01953 Position (kb) 2017 2018 2019 2020Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 2016.616 kb on + strand, within ECD_01951at 2016.772 kb on + strand, within ECD_01951at 2016.773 kb on - strand, within ECD_01951at 2016.773 kb on - strand, within ECD_01951at 2017.073 kb on + strand, within ECD_01951at 2017.457 kb on + strandat 2017.458 kb on - strandat 2017.473 kb on + strandat 2017.592 kb on - strandat 2017.837 kb on - strand, within ECD_01952at 2017.837 kb on - strand, within ECD_01952at 2017.881 kb on + strand, within ECD_01952at 2017.901 kb on - strand, within ECD_01952at 2017.961 kb on - strand, within ECD_01952at 2018.107 kb on + strand, within ECD_01952at 2018.217 kb on + strand, within ECD_01952at 2018.218 kb on - strand, within ECD_01952at 2018.340 kb on + strand, within ECD_01952at 2018.399 kb on - strand, within ECD_01952at 2018.835 kb on + strand, within ECD_01952at 2018.846 kb on - strand, within ECD_01952at 2019.069 kb on - strandat 2019.230 kb on + strand, within ECD_01953at 2019.281 kb on - strand, within ECD_01953at 2019.914 kb on + strand, within ECD_01953

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Per-strain Table

Position Strand Gene LocusTag Fraction P2_phage_0.5625_MOI
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2,016,616 + ECD_01951 0.36 +3.8
2,016,772 + ECD_01951 0.48 -0.7
2,016,773 - ECD_01951 0.48 +3.1
2,016,773 - ECD_01951 0.48 -1.3
2,017,073 + ECD_01951 0.72 +1.7
2,017,457 + -1.6
2,017,458 - -1.4
2,017,473 + +3.3
2,017,592 - +0.6
2,017,837 - ECD_01952 0.16 -0.3
2,017,837 - ECD_01952 0.16 -1.5
2,017,881 + ECD_01952 0.19 -0.5
2,017,901 - ECD_01952 0.20 +1.2
2,017,961 - ECD_01952 0.25 -0.3
2,018,107 + ECD_01952 0.34 +0.0
2,018,217 + ECD_01952 0.42 -0.7
2,018,218 - ECD_01952 0.42 -1.2
2,018,340 + ECD_01952 0.50 -1.1
2,018,399 - ECD_01952 0.54 +0.3
2,018,835 + ECD_01952 0.84 -0.6
2,018,846 - ECD_01952 0.84 -1.9
2,019,069 - +0.3
2,019,230 + ECD_01953 0.11 -0.4
2,019,281 - ECD_01953 0.15 -0.0
2,019,914 + ECD_01953 0.60 -1.3

Or see this region's nucleotide sequence