Strain Fitness in Escherichia coli BL21 around ECD_01950

Experiment: P2_phage_0.5625_MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01949 and ECD_01950 are separated by 10 nucleotidesECD_01950 and ECD_01951 overlap by 4 nucleotides ECD_01949: ECD_01949 - colanic acid biosynthesis protein, at 2,013,534 to 2,014,928 _01949 ECD_01950: ECD_01950 - putative glycosyl transferase, at 2,014,939 to 2,016,159 _01950 ECD_01951: ECD_01951 - colanic acid biosynthesis protein, at 2,016,156 to 2,017,436 _01951 Position (kb) 2014 2015 2016 2017Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 2014.507 kb on + strand, within ECD_01949at 2014.862 kb on + strandat 2014.929 kb on - strandat 2014.929 kb on - strandat 2014.940 kb on + strandat 2015.669 kb on + strand, within ECD_01950at 2015.670 kb on - strand, within ECD_01950at 2016.079 kb on - strandat 2016.085 kb on - strandat 2016.376 kb on + strand, within ECD_01951at 2016.377 kb on - strand, within ECD_01951at 2016.377 kb on - strand, within ECD_01951at 2016.574 kb on + strand, within ECD_01951at 2016.574 kb on + strand, within ECD_01951at 2016.616 kb on + strand, within ECD_01951at 2016.772 kb on + strand, within ECD_01951at 2016.773 kb on - strand, within ECD_01951at 2016.773 kb on - strand, within ECD_01951at 2017.073 kb on + strand, within ECD_01951

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Per-strain Table

Position Strand Gene LocusTag Fraction P2_phage_0.5625_MOI
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2,014,507 + ECD_01949 0.70 -0.7
2,014,862 + -1.1
2,014,929 - -2.4
2,014,929 - -0.4
2,014,940 + +2.1
2,015,669 + ECD_01950 0.60 -2.0
2,015,670 - ECD_01950 0.60 -0.2
2,016,079 - -0.2
2,016,085 - -0.2
2,016,376 + ECD_01951 0.17 -1.8
2,016,377 - ECD_01951 0.17 -0.8
2,016,377 - ECD_01951 0.17 -0.8
2,016,574 + ECD_01951 0.33 -2.1
2,016,574 + ECD_01951 0.33 -0.7
2,016,616 + ECD_01951 0.36 +3.8
2,016,772 + ECD_01951 0.48 -0.7
2,016,773 - ECD_01951 0.48 +3.1
2,016,773 - ECD_01951 0.48 -1.3
2,017,073 + ECD_01951 0.72 +1.7

Or see this region's nucleotide sequence