Strain Fitness in Escherichia coli BL21 around ECD_01803

Experiment: P2_phage_0.5625_MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01801 and ECD_01802 are separated by 19 nucleotidesECD_01802 and ECD_01803 are separated by 96 nucleotidesECD_01803 and ECD_01804 are separated by 75 nucleotides ECD_01801: ECD_01801 - carboxy-terminal protease for penicillin-binding protein 3, at 1,858,493 to 1,860,541 _01801 ECD_01802: ECD_01802 - RNA chaperone, putative ProP translation regulator, at 1,860,561 to 1,861,259 _01802 ECD_01803: ECD_01803 - free methionine-(R)-sulfoxide reductase, at 1,861,356 to 1,861,907 _01803 ECD_01804: ECD_01804 - inner membrane PqiA domain protein, at 1,861,983 to 1,863,266 _01804 Position (kb) 1861 1862Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1860.467 kb on - strandat 1860.474 kb on - strandat 1860.642 kb on + strand, within ECD_01802at 1860.646 kb on - strand, within ECD_01802at 1860.973 kb on - strand, within ECD_01802at 1860.973 kb on - strand, within ECD_01802at 1860.973 kb on - strand, within ECD_01802at 1860.973 kb on - strand, within ECD_01802at 1860.973 kb on - strand, within ECD_01802at 1860.973 kb on - strand, within ECD_01802at 1860.973 kb on - strand, within ECD_01802at 1861.070 kb on - strand, within ECD_01802at 1861.090 kb on - strand, within ECD_01802at 1861.245 kb on - strandat 1861.245 kb on - strandat 1861.245 kb on - strandat 1861.273 kb on + strandat 1861.284 kb on + strandat 1861.284 kb on + strandat 1861.318 kb on - strandat 1861.318 kb on - strandat 1861.318 kb on - strandat 1861.319 kb on + strandat 1861.320 kb on - strandat 1861.320 kb on - strandat 1861.320 kb on - strandat 1861.341 kb on + strandat 1861.342 kb on - strandat 1861.435 kb on - strand, within ECD_01803at 1861.435 kb on - strand, within ECD_01803at 1861.435 kb on - strand, within ECD_01803at 1861.476 kb on - strand, within ECD_01803at 1861.562 kb on + strand, within ECD_01803at 1861.729 kb on - strand, within ECD_01803at 1861.748 kb on - strand, within ECD_01803at 1861.748 kb on - strand, within ECD_01803at 1861.748 kb on - strand, within ECD_01803at 1861.857 kb on - strandat 1861.908 kb on + strandat 1862.263 kb on + strand, within ECD_01804at 1862.394 kb on + strand, within ECD_01804at 1862.815 kb on + strand, within ECD_01804at 1862.834 kb on + strand, within ECD_01804at 1862.835 kb on - strand, within ECD_01804

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Per-strain Table

Position Strand Gene LocusTag Fraction P2_phage_0.5625_MOI
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1,860,467 - +2.1
1,860,474 - +2.4
1,860,642 + ECD_01802 0.12 -0.3
1,860,646 - ECD_01802 0.12 +0.8
1,860,973 - ECD_01802 0.59 +1.7
1,860,973 - ECD_01802 0.59 +3.2
1,860,973 - ECD_01802 0.59 +0.7
1,860,973 - ECD_01802 0.59 -1.0
1,860,973 - ECD_01802 0.59 +1.3
1,860,973 - ECD_01802 0.59 +1.5
1,860,973 - ECD_01802 0.59 +0.3
1,861,070 - ECD_01802 0.73 -1.2
1,861,090 - ECD_01802 0.76 +1.0
1,861,245 - +2.2
1,861,245 - -1.7
1,861,245 - +1.4
1,861,273 + +1.5
1,861,284 + -0.4
1,861,284 + +1.4
1,861,318 - -0.6
1,861,318 - -0.0
1,861,318 - -0.1
1,861,319 + +0.3
1,861,320 - +1.3
1,861,320 - +3.1
1,861,320 - +1.8
1,861,341 + +1.0
1,861,342 - +3.0
1,861,435 - ECD_01803 0.14 +1.6
1,861,435 - ECD_01803 0.14 +1.1
1,861,435 - ECD_01803 0.14 -0.7
1,861,476 - ECD_01803 0.22 +0.6
1,861,562 + ECD_01803 0.37 +0.9
1,861,729 - ECD_01803 0.68 +2.2
1,861,748 - ECD_01803 0.71 +1.6
1,861,748 - ECD_01803 0.71 +1.2
1,861,748 - ECD_01803 0.71 +2.4
1,861,857 - +1.3
1,861,908 + +2.3
1,862,263 + ECD_01804 0.22 +1.9
1,862,394 + ECD_01804 0.32 -1.9
1,862,815 + ECD_01804 0.65 -0.1
1,862,834 + ECD_01804 0.66 +1.5
1,862,835 - ECD_01804 0.66 +0.8

Or see this region's nucleotide sequence