Experiment: P2_phage_0.5625_MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECD_01774 and ECD_01775 are separated by 69 nucleotides ECD_01775 and ECD_01776 are separated by 204 nucleotides ECD_01776 and ECD_01777 are separated by 39 nucleotides
ECD_01774: ECD_01774 - ribonuclease D, at 1,832,590 to 1,833,717
_01774
ECD_01775: ECD_01775 - acyl-CoA synthetase (long-chain-fatty-acid--CoA ligase), at 1,833,787 to 1,835,472
_01775
ECD_01776: ECD_01776 - Slp family lipoprotein, RpoE-regulated, at 1,835,677 to 1,836,258
_01776
ECD_01777: ECD_01777 - tRNA(ANN) t(6)A37 threonylcarbamoyladenosine modification protein; binding partner and protease for TsaD, at 1,836,298 to 1,836,993
_01777
Position (kb) 1833
1834
1835
1836 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1833.220 kb on - strand, within ECD_01774 at 1833.557 kb on - strand, within ECD_01774 at 1834.034 kb on - strand, within ECD_01775 at 1834.084 kb on - strand, within ECD_01775 at 1834.201 kb on - strand, within ECD_01775 at 1835.329 kb on + strand at 1835.601 kb on - strand at 1836.108 kb on - strand, within ECD_01776 at 1836.227 kb on - strand at 1836.227 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P2_phage_0.5625_MOI remove 1,833,220 - ECD_01774 0.56 +0.4 1,833,557 - ECD_01774 0.86 -2.3 1,834,034 - ECD_01775 0.15 -1.2 1,834,084 - ECD_01775 0.18 -0.4 1,834,201 - ECD_01775 0.25 +0.1 1,835,329 + +2.6 1,835,601 - -1.1 1,836,108 - ECD_01776 0.74 +0.8 1,836,227 - +1.5 1,836,227 - +3.0
Or see this region's nucleotide sequence