Strain Fitness in Escherichia coli BL21 around ECD_01273

Experiment: P2_phage_0.5625_MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01271 and ECD_01272 are separated by 311 nucleotidesECD_01272 and ECD_01273 are separated by 133 nucleotidesECD_01273 and ECD_01274 are separated by 302 nucleotides ECD_01271: ECD_01271 - antimicrobial peptide transport ABC transporter periplasmic binding protein, at 1,341,834 to 1,343,477 _01271 ECD_01272: ECD_01272 - DUF2543 family protein, at 1,343,789 to 1,344,034 _01272 ECD_01273: ECD_01273 - putrescine importer, at 1,344,168 to 1,345,553 _01273 ECD_01274: ECD_01274 - glutamate--putrescine ligase, at 1,345,856 to 1,347,274 _01274 Position (kb) 1344 1345 1346Strain fitness (log2 ratio) -1 0 1 2 3 4at 1343.640 kb on - strandat 1343.640 kb on - strandat 1343.846 kb on - strand, within ECD_01272at 1344.203 kb on - strandat 1344.245 kb on - strandat 1344.707 kb on - strand, within ECD_01273at 1344.812 kb on - strand, within ECD_01273at 1345.292 kb on + strand, within ECD_01273at 1345.454 kb on - strandat 1345.696 kb on - strandat 1345.732 kb on + strandat 1345.965 kb on - strandat 1346.203 kb on + strand, within ECD_01274at 1346.204 kb on - strand, within ECD_01274at 1346.257 kb on - strand, within ECD_01274at 1346.257 kb on - strand, within ECD_01274at 1346.257 kb on - strand, within ECD_01274at 1346.257 kb on - strand, within ECD_01274at 1346.257 kb on - strand, within ECD_01274at 1346.471 kb on - strand, within ECD_01274

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Per-strain Table

Position Strand Gene LocusTag Fraction P2_phage_0.5625_MOI
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1,343,640 - -1.3
1,343,640 - +1.5
1,343,846 - ECD_01272 0.23 +1.7
1,344,203 - +1.8
1,344,245 - +2.1
1,344,707 - ECD_01273 0.39 +0.2
1,344,812 - ECD_01273 0.46 -0.1
1,345,292 + ECD_01273 0.81 -0.4
1,345,454 - +3.8
1,345,696 - -0.9
1,345,732 + +3.1
1,345,965 - +0.1
1,346,203 + ECD_01274 0.24 -0.7
1,346,204 - ECD_01274 0.25 +1.0
1,346,257 - ECD_01274 0.28 +3.5
1,346,257 - ECD_01274 0.28 +1.4
1,346,257 - ECD_01274 0.28 -0.2
1,346,257 - ECD_01274 0.28 +0.3
1,346,257 - ECD_01274 0.28 +2.1
1,346,471 - ECD_01274 0.43 -0.4

Or see this region's nucleotide sequence