Strain Fitness in Escherichia coli BL21 around ECD_01223

Experiment: P2_phage_0.5625_MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01221 and ECD_01222 are separated by 52 nucleotidesECD_01222 and ECD_01223 are separated by 34 nucleotidesECD_01223 and ECD_04308 are separated by 31 nucleotidesECD_04308 and ECD_01224 are separated by 54 nucleotides ECD_01221: ECD_01221 - oligopeptide ABC transporter ATPase, at 1,291,356 to 1,292,360 _01221 ECD_01222: ECD_01222 - UPF0263 family protein, at 1,292,413 to 1,292,742 _01222 ECD_01223: ECD_01223 - cardiolipin synthase 1, at 1,292,777 to 1,294,237 _01223 ECD_04308: ECD_04308 - uncharacterized protein, at 1,294,269 to 1,294,553 _04308 ECD_01224: ECD_01224 - voltage-gated potassium channel, at 1,294,608 to 1,295,861 _01224 Position (kb) 1292 1293 1294 1295Strain fitness (log2 ratio) -1 0 1 2at 1292.100 kb on + strand, within ECD_01221at 1292.218 kb on + strand, within ECD_01221at 1293.189 kb on - strand, within ECD_01223at 1293.647 kb on - strand, within ECD_01223at 1293.968 kb on - strand, within ECD_01223at 1293.974 kb on - strand, within ECD_01223at 1294.360 kb on + strand, within ECD_04308at 1294.367 kb on + strand, within ECD_04308at 1294.372 kb on + strand, within ECD_04308at 1294.372 kb on + strand, within ECD_04308at 1294.557 kb on + strandat 1294.588 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction P2_phage_0.5625_MOI
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1,292,100 + ECD_01221 0.74 -0.8
1,292,218 + ECD_01221 0.86 -1.4
1,293,189 - ECD_01223 0.28 +0.9
1,293,647 - ECD_01223 0.60 +0.8
1,293,968 - ECD_01223 0.82 -0.8
1,293,974 - ECD_01223 0.82 -0.0
1,294,360 + ECD_04308 0.32 +2.6
1,294,367 + ECD_04308 0.34 +1.8
1,294,372 + ECD_04308 0.36 +2.0
1,294,372 + ECD_04308 0.36 +1.6
1,294,557 + +0.5
1,294,588 + -0.2

Or see this region's nucleotide sequence