Experiment: P2_phage_0.5625_MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECD_01159 and ECD_01160 are separated by 45 nucleotides ECD_01160 and ECD_01161 are separated by 145 nucleotides ECD_01161 and ECD_01162 are separated by 220 nucleotides ECD_01162 and ECD_01163 are separated by 51 nucleotides
ECD_01159: ECD_01159 - translesion error-prone DNA polymerase V subunit; DNA polymerase activity, at 1,219,334 to 1,220,602
_01159
ECD_01160: ECD_01160 - oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I, at 1,220,648 to 1,221,178
_01160
ECD_01161: ECD_01161 - sodium:proton antiporter, at 1,221,324 to 1,222,865
_01161
ECD_01162: ECD_01162 - fatty acid metabolism regulon transcriptional regulator, at 1,223,086 to 1,223,805
_01162
ECD_01163: ECD_01163 - SpoVR family stationary phase protein, at 1,223,857 to 1,225,389
_01163
Position (kb)
1221
1222
1223 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1220.567 kb on - strand at 1220.918 kb on + strand, within ECD_01160 at 1220.919 kb on - strand, within ECD_01160 at 1220.942 kb on - strand, within ECD_01160 at 1220.943 kb on + strand, within ECD_01160 at 1220.943 kb on + strand, within ECD_01160 at 1220.944 kb on - strand, within ECD_01160 at 1220.944 kb on - strand, within ECD_01160 at 1220.944 kb on - strand, within ECD_01160 at 1220.967 kb on + strand, within ECD_01160 at 1221.042 kb on + strand, within ECD_01160 at 1221.047 kb on - strand, within ECD_01160 at 1221.304 kb on - strand at 1221.425 kb on - strand at 1221.632 kb on - strand, within ECD_01161 at 1222.102 kb on - strand, within ECD_01161 at 1222.382 kb on - strand, within ECD_01161 at 1222.501 kb on - strand, within ECD_01161 at 1222.519 kb on - strand, within ECD_01161 at 1222.546 kb on + strand, within ECD_01161 at 1222.682 kb on - strand, within ECD_01161 at 1223.026 kb on + strand at 1223.172 kb on + strand, within ECD_01162 at 1223.528 kb on + strand, within ECD_01162 at 1223.662 kb on + strand, within ECD_01162
Per-strain Table
Position Strand Gene LocusTag Fraction P2_phage_0.5625_MOI remove 1,220,567 - +0.3 1,220,918 + ECD_01160 0.51 +0.3 1,220,919 - ECD_01160 0.51 +3.2 1,220,942 - ECD_01160 0.55 +1.8 1,220,943 + ECD_01160 0.56 -0.7 1,220,943 + ECD_01160 0.56 +2.9 1,220,944 - ECD_01160 0.56 +1.9 1,220,944 - ECD_01160 0.56 +1.2 1,220,944 - ECD_01160 0.56 +0.3 1,220,967 + ECD_01160 0.60 +3.3 1,221,042 + ECD_01160 0.74 -1.2 1,221,047 - ECD_01160 0.75 +2.1 1,221,304 - -1.2 1,221,425 - +0.1 1,221,632 - ECD_01161 0.20 -0.1 1,222,102 - ECD_01161 0.50 -1.3 1,222,382 - ECD_01161 0.69 -1.1 1,222,501 - ECD_01161 0.76 -1.3 1,222,519 - ECD_01161 0.77 +0.9 1,222,546 + ECD_01161 0.79 +1.9 1,222,682 - ECD_01161 0.88 +1.1 1,223,026 + -0.3 1,223,172 + ECD_01162 0.12 -0.1 1,223,528 + ECD_01162 0.61 -0.1 1,223,662 + ECD_01162 0.80 -0.7
Or see this region's nucleotide sequence