Strain Fitness in Escherichia coli BL21 around ECD_01161

Experiment: P2_phage_0.5625_MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01159 and ECD_01160 are separated by 45 nucleotidesECD_01160 and ECD_01161 are separated by 145 nucleotidesECD_01161 and ECD_01162 are separated by 220 nucleotidesECD_01162 and ECD_01163 are separated by 51 nucleotides ECD_01159: ECD_01159 - translesion error-prone DNA polymerase V subunit; DNA polymerase activity, at 1,219,334 to 1,220,602 _01159 ECD_01160: ECD_01160 - oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I, at 1,220,648 to 1,221,178 _01160 ECD_01161: ECD_01161 - sodium:proton antiporter, at 1,221,324 to 1,222,865 _01161 ECD_01162: ECD_01162 - fatty acid metabolism regulon transcriptional regulator, at 1,223,086 to 1,223,805 _01162 ECD_01163: ECD_01163 - SpoVR family stationary phase protein, at 1,223,857 to 1,225,389 _01163 Position (kb) 1221 1222 1223Strain fitness (log2 ratio) -1 0 1 2 3at 1220.567 kb on - strandat 1220.918 kb on + strand, within ECD_01160at 1220.919 kb on - strand, within ECD_01160at 1220.942 kb on - strand, within ECD_01160at 1220.943 kb on + strand, within ECD_01160at 1220.943 kb on + strand, within ECD_01160at 1220.944 kb on - strand, within ECD_01160at 1220.944 kb on - strand, within ECD_01160at 1220.944 kb on - strand, within ECD_01160at 1220.967 kb on + strand, within ECD_01160at 1221.042 kb on + strand, within ECD_01160at 1221.047 kb on - strand, within ECD_01160at 1221.304 kb on - strandat 1221.425 kb on - strandat 1221.632 kb on - strand, within ECD_01161at 1222.102 kb on - strand, within ECD_01161at 1222.382 kb on - strand, within ECD_01161at 1222.501 kb on - strand, within ECD_01161at 1222.519 kb on - strand, within ECD_01161at 1222.546 kb on + strand, within ECD_01161at 1222.682 kb on - strand, within ECD_01161at 1223.026 kb on + strandat 1223.172 kb on + strand, within ECD_01162at 1223.528 kb on + strand, within ECD_01162at 1223.662 kb on + strand, within ECD_01162

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Per-strain Table

Position Strand Gene LocusTag Fraction P2_phage_0.5625_MOI
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1,220,567 - +0.3
1,220,918 + ECD_01160 0.51 +0.3
1,220,919 - ECD_01160 0.51 +3.2
1,220,942 - ECD_01160 0.55 +1.8
1,220,943 + ECD_01160 0.56 -0.7
1,220,943 + ECD_01160 0.56 +2.9
1,220,944 - ECD_01160 0.56 +1.9
1,220,944 - ECD_01160 0.56 +1.2
1,220,944 - ECD_01160 0.56 +0.3
1,220,967 + ECD_01160 0.60 +3.3
1,221,042 + ECD_01160 0.74 -1.2
1,221,047 - ECD_01160 0.75 +2.1
1,221,304 - -1.2
1,221,425 - +0.1
1,221,632 - ECD_01161 0.20 -0.1
1,222,102 - ECD_01161 0.50 -1.3
1,222,382 - ECD_01161 0.69 -1.1
1,222,501 - ECD_01161 0.76 -1.3
1,222,519 - ECD_01161 0.77 +0.9
1,222,546 + ECD_01161 0.79 +1.9
1,222,682 - ECD_01161 0.88 +1.1
1,223,026 + -0.3
1,223,172 + ECD_01162 0.12 -0.1
1,223,528 + ECD_01162 0.61 -0.1
1,223,662 + ECD_01162 0.80 -0.7

Or see this region's nucleotide sequence