Experiment: P2_phage_0.5625_MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECD_01121 and ECD_01122 overlap by 4 nucleotides ECD_01122 and ECD_01123 overlap by 4 nucleotides ECD_01123 and ECD_01124 are separated by 13 nucleotides
ECD_01121: ECD_01121 - spermidine/putrescine ABC transporter periplasmic binding protein, at 1,185,151 to 1,186,197
_01121
ECD_01122: ECD_01122 - spermidine/putrescine ABC transporter permease, at 1,186,194 to 1,186,988
_01122
ECD_01123: ECD_01123 - spermidine/putrescine ABC transporter permease, at 1,186,985 to 1,187,812
_01123
ECD_01124: ECD_01124 - spermidine/putrescine ABC transporter ATPase, at 1,187,826 to 1,188,962
_01124
Position (kb) 1186
1187 Strain fitness (log2 ratio) -1
0
1
2
3 at 1185.461 kb on - strand, within ECD_01121 at 1185.478 kb on - strand, within ECD_01121 at 1185.546 kb on - strand, within ECD_01121 at 1185.612 kb on - strand, within ECD_01121 at 1185.811 kb on - strand, within ECD_01121 at 1185.945 kb on - strand, within ECD_01121 at 1185.945 kb on - strand, within ECD_01121 at 1186.002 kb on - strand, within ECD_01121 at 1186.002 kb on - strand, within ECD_01121 at 1186.023 kb on - strand, within ECD_01121 at 1186.104 kb on - strand at 1186.110 kb on - strand at 1186.282 kb on - strand, within ECD_01122 at 1186.417 kb on - strand, within ECD_01122 at 1186.584 kb on - strand, within ECD_01122 at 1186.934 kb on - strand at 1187.176 kb on - strand, within ECD_01123 at 1187.649 kb on - strand, within ECD_01123
Per-strain Table
Position Strand Gene LocusTag Fraction P2_phage_0.5625_MOI remove 1,185,461 - ECD_01121 0.30 +1.6 1,185,478 - ECD_01121 0.31 +2.6 1,185,546 - ECD_01121 0.38 +1.3 1,185,612 - ECD_01121 0.44 +1.3 1,185,811 - ECD_01121 0.63 -0.3 1,185,945 - ECD_01121 0.76 +0.9 1,185,945 - ECD_01121 0.76 -0.2 1,186,002 - ECD_01121 0.81 +2.8 1,186,002 - ECD_01121 0.81 -0.9 1,186,023 - ECD_01121 0.83 +0.6 1,186,104 - +1.7 1,186,110 - -0.6 1,186,282 - ECD_01122 0.11 -1.7 1,186,417 - ECD_01122 0.28 +2.1 1,186,584 - ECD_01122 0.49 +1.9 1,186,934 - -0.4 1,187,176 - ECD_01123 0.23 -0.3 1,187,649 - ECD_01123 0.80 +0.7
Or see this region's nucleotide sequence