Strain Fitness in Escherichia coli BL21 around ECD_01109

Experiment: P2_phage_0.5625_MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01107 and ECD_01108 are separated by 240 nucleotidesECD_01108 and ECD_01109 are separated by 82 nucleotidesECD_01109 and ECD_01110 are separated by 143 nucleotides ECD_01107: ECD_01107 - repressor for bhsA(ycfR), at 1,170,792 to 1,171,424 _01107 ECD_01108: ECD_01108 - biofilm, cell surface and signaling protein, at 1,171,665 to 1,171,922 _01108 ECD_01109: ECD_01109 - L,D-transpeptidase linking Lpp to murein, at 1,172,005 to 1,172,967 _01109 ECD_01110: ECD_01110 - transcription-repair coupling factor, at 1,173,111 to 1,176,557 _01110 Position (kb) 1172 1173Strain fitness (log2 ratio) -1 0 1 2 3at 1171.258 kb on - strand, within ECD_01107at 1172.570 kb on - strand, within ECD_01109at 1172.624 kb on + strand, within ECD_01109at 1172.627 kb on + strand, within ECD_01109at 1172.627 kb on + strand, within ECD_01109at 1173.471 kb on - strand, within ECD_01110at 1173.633 kb on - strand, within ECD_01110at 1173.634 kb on + strand, within ECD_01110at 1173.635 kb on - strand, within ECD_01110at 1173.635 kb on - strand, within ECD_01110at 1173.635 kb on - strand, within ECD_01110at 1173.635 kb on - strand, within ECD_01110

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Per-strain Table

Position Strand Gene LocusTag Fraction P2_phage_0.5625_MOI
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1,171,258 - ECD_01107 0.74 +0.1
1,172,570 - ECD_01109 0.59 -1.2
1,172,624 + ECD_01109 0.64 -0.4
1,172,627 + ECD_01109 0.65 +1.6
1,172,627 + ECD_01109 0.65 -0.3
1,173,471 - ECD_01110 0.10 -0.0
1,173,633 - ECD_01110 0.15 -0.0
1,173,634 + ECD_01110 0.15 -0.1
1,173,635 - ECD_01110 0.15 +2.9
1,173,635 - ECD_01110 0.15 +1.8
1,173,635 - ECD_01110 0.15 +2.9
1,173,635 - ECD_01110 0.15 +0.9

Or see this region's nucleotide sequence