Strain Fitness in Escherichia coli BL21 around ECD_01105

Experiment: P2_phage_0.5625_MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01103 and ECD_01104 are separated by 22 nucleotidesECD_01104 and ECD_01105 are separated by 407 nucleotidesECD_01105 and ECD_01106 are separated by 226 nucleotidesECD_01106 and ECD_01107 are separated by 61 nucleotides ECD_01103: ECD_01103 - beta N-acetyl-glucosaminidase, at 1,166,662 to 1,167,687 _01103 ECD_01104: ECD_01104 - putative UPF0227 family esterase, at 1,167,710 to 1,168,252 _01104 ECD_01105: ECD_01105 - respiratory NADH dehydrogenase 2/cupric reductase, at 1,168,660 to 1,169,964 _01105 ECD_01106: ECD_01106 - uncharacterized protein, at 1,170,191 to 1,170,730 _01106 ECD_01107: ECD_01107 - repressor for bhsA(ycfR), at 1,170,792 to 1,171,424 _01107 Position (kb) 1168 1169 1170Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 1167.697 kb on - strandat 1167.795 kb on + strand, within ECD_01104at 1167.804 kb on + strand, within ECD_01104at 1168.417 kb on - strandat 1168.767 kb on + strandat 1169.207 kb on + strand, within ECD_01105at 1169.291 kb on + strand, within ECD_01105at 1169.300 kb on + strand, within ECD_01105at 1169.316 kb on + strand, within ECD_01105at 1169.340 kb on + strand, within ECD_01105at 1169.340 kb on + strand, within ECD_01105at 1169.341 kb on - strand, within ECD_01105at 1169.529 kb on + strand, within ECD_01105at 1169.590 kb on + strand, within ECD_01105at 1169.691 kb on + strand, within ECD_01105at 1169.846 kb on + strandat 1169.848 kb on + strandat 1169.850 kb on + strandat 1169.850 kb on + strandat 1169.860 kb on + strandat 1169.906 kb on + strandat 1169.925 kb on + strandat 1169.971 kb on + strandat 1170.019 kb on + strandat 1170.894 kb on - strand, within ECD_01107

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Per-strain Table

Position Strand Gene LocusTag Fraction P2_phage_0.5625_MOI
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1,167,697 - +4.7
1,167,795 + ECD_01104 0.16 +2.4
1,167,804 + ECD_01104 0.17 -0.3
1,168,417 - +0.2
1,168,767 + +0.3
1,169,207 + ECD_01105 0.42 -0.3
1,169,291 + ECD_01105 0.48 -1.1
1,169,300 + ECD_01105 0.49 +0.4
1,169,316 + ECD_01105 0.50 -1.4
1,169,340 + ECD_01105 0.52 -0.5
1,169,340 + ECD_01105 0.52 -0.7
1,169,341 - ECD_01105 0.52 -0.8
1,169,529 + ECD_01105 0.67 -1.8
1,169,590 + ECD_01105 0.71 -1.5
1,169,691 + ECD_01105 0.79 -2.6
1,169,846 + -0.7
1,169,848 + +0.3
1,169,850 + +1.1
1,169,850 + -0.9
1,169,860 + -0.9
1,169,906 + +1.8
1,169,925 + +0.0
1,169,971 + -0.2
1,170,019 + +1.6
1,170,894 - ECD_01107 0.16 -0.7

Or see this region's nucleotide sequence