Strain Fitness in Escherichia coli BL21 around ECD_01070

Experiment: P2_phage_0.5625_MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_01068 and ECD_01069 are separated by 154 nucleotidesECD_01069 and ECD_01070 are separated by 3 nucleotidesECD_01070 and ECD_01071 are separated by 11 nucleotidesECD_01071 and ECD_01072 are separated by 24 nucleotides ECD_01068: ECD_01068 - assembly protein for flagellar basal-body periplasmic P ring, at 1,132,774 to 1,133,433 _01068 ECD_01069: ECD_01069 - flagellar component of cell-proximal portion of basal-body rod, at 1,133,588 to 1,134,004 _01069 ECD_01070: ECD_01070 - flagellar component of cell-proximal portion of basal-body rod, at 1,134,008 to 1,134,412 _01070 ECD_01071: ECD_01071 - flagellar hook assembly protein, at 1,134,424 to 1,135,119 _01071 ECD_01072: ECD_01072 - flagellar hook protein, at 1,135,144 to 1,136,349 _01072 Position (kb) 1134 1135Strain fitness (log2 ratio) -2 -1 0 1at 1133.145 kb on - strand, within ECD_01068at 1133.162 kb on - strand, within ECD_01068at 1133.178 kb on - strand, within ECD_01068at 1133.290 kb on - strand, within ECD_01068at 1133.381 kb on - strandat 1133.454 kb on - strandat 1133.467 kb on + strandat 1133.500 kb on - strandat 1133.572 kb on + strandat 1133.587 kb on + strandat 1133.745 kb on + strand, within ECD_01069at 1133.745 kb on + strand, within ECD_01069at 1133.846 kb on + strand, within ECD_01069at 1133.878 kb on + strand, within ECD_01069at 1133.933 kb on + strand, within ECD_01069at 1134.150 kb on + strand, within ECD_01070at 1134.410 kb on + strandat 1134.423 kb on + strandat 1134.483 kb on + strandat 1134.876 kb on + strand, within ECD_01071at 1134.965 kb on + strand, within ECD_01071at 1134.965 kb on + strand, within ECD_01071at 1135.078 kb on + strandat 1135.078 kb on + strandat 1135.112 kb on + strandat 1135.239 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction P2_phage_0.5625_MOI
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1,133,145 - ECD_01068 0.56 -0.9
1,133,162 - ECD_01068 0.59 +1.5
1,133,178 - ECD_01068 0.61 -2.1
1,133,290 - ECD_01068 0.78 -0.2
1,133,381 - -0.6
1,133,454 - -1.4
1,133,467 + +1.7
1,133,500 - -1.6
1,133,572 + -0.0
1,133,587 + +1.3
1,133,745 + ECD_01069 0.38 -0.6
1,133,745 + ECD_01069 0.38 -0.5
1,133,846 + ECD_01069 0.62 +1.7
1,133,878 + ECD_01069 0.70 +1.7
1,133,933 + ECD_01069 0.83 -0.2
1,134,150 + ECD_01070 0.35 +1.6
1,134,410 + -1.3
1,134,423 + +1.4
1,134,483 + +1.0
1,134,876 + ECD_01071 0.65 +0.7
1,134,965 + ECD_01071 0.78 +1.1
1,134,965 + ECD_01071 0.78 +0.0
1,135,078 + +0.6
1,135,078 + -1.6
1,135,112 + +0.2
1,135,239 + -2.2

Or see this region's nucleotide sequence