Experiment: P2_phage_0.5625_MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECD_01021 and ECD_01022 are separated by 344 nucleotides ECD_01022 and ECD_01023 are separated by 49 nucleotides ECD_01023 and ECD_01024 are separated by 1 nucleotides
ECD_01021: ECD_01021 - deferrrochelatase, periplasmic, at 1,087,659 to 1,088,930
_01021
ECD_01022: ECD_01022 - ATP-binding protein; putative PhoH family P-loop ATPase, at 1,089,275 to 1,090,339
_01022
ECD_01023: ECD_01023 - biofilm PGA synthase PgaCD, regulatory subunit; c-di-GMP-stimulated activity and dimerization, at 1,090,389 to 1,090,802
_01023
ECD_01024: ECD_01024 - biofilm PGA synthase PgaCD, catalytic subunit; poly-beta-1,6-N-acetyl-D-glucosamine synthase, at 1,090,804 to 1,092,129
_01024
Position (kb) 1089
1090
1091 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1088.321 kb on + strand, within ECD_01021 at 1088.463 kb on + strand, within ECD_01021 at 1088.473 kb on + strand, within ECD_01021 at 1088.712 kb on + strand, within ECD_01021 at 1089.463 kb on - strand, within ECD_01022 at 1089.625 kb on + strand, within ECD_01022 at 1089.853 kb on + strand, within ECD_01022 at 1089.853 kb on + strand, within ECD_01022
Per-strain Table
Position Strand Gene LocusTag Fraction P2_phage_0.5625_MOI remove 1,088,321 + ECD_01021 0.52 +1.1 1,088,463 + ECD_01021 0.63 +1.8 1,088,473 + ECD_01021 0.64 -1.8 1,088,712 + ECD_01021 0.83 -1.3 1,089,463 - ECD_01022 0.18 -1.2 1,089,625 + ECD_01022 0.33 +1.8 1,089,853 + ECD_01022 0.54 -2.2 1,089,853 + ECD_01022 0.54 +0.1
Or see this region's nucleotide sequence