Strain Fitness in Escherichia coli BL21 around ECD_00775

Experiment: P2_phage_0.5625_MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00774 and ECD_00775 overlap by 4 nucleotidesECD_00775 and ECD_00776 are separated by 260 nucleotidesECD_00776 and ECD_00777 overlap by 4 nucleotides ECD_00774: ECD_00774 - 23S rRNA m(6)A1618 methyltransferase, SAM-dependent, at 826,324 to 827,250 _00774 ECD_00775: ECD_00775 - mechanosensitive channel protein, intermediate conductance, at 827,247 to 829,472 _00775 ECD_00776: ECD_00776 - glutamine transporter subunit, at 829,733 to 830,455 _00776 ECD_00777: ECD_00777 - glutamine transporter subunit, at 830,452 to 831,111 _00777 Position (kb) 827 828 829 830Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 826.269 kb on - strandat 826.323 kb on + strandat 826.672 kb on - strand, within ECD_00774at 826.699 kb on + strand, within ECD_00774at 827.102 kb on + strand, within ECD_00774at 827.248 kb on + strandat 827.315 kb on - strandat 827.656 kb on + strand, within ECD_00775at 827.677 kb on - strand, within ECD_00775at 827.790 kb on - strand, within ECD_00775at 827.832 kb on + strand, within ECD_00775at 827.943 kb on - strand, within ECD_00775at 828.242 kb on + strand, within ECD_00775at 828.243 kb on - strand, within ECD_00775at 828.243 kb on - strand, within ECD_00775at 828.252 kb on - strand, within ECD_00775at 828.252 kb on - strand, within ECD_00775at 828.252 kb on - strand, within ECD_00775at 828.524 kb on + strand, within ECD_00775at 828.598 kb on - strand, within ECD_00775at 828.598 kb on - strand, within ECD_00775at 828.648 kb on + strand, within ECD_00775at 828.649 kb on - strand, within ECD_00775at 828.753 kb on + strand, within ECD_00775at 828.754 kb on - strand, within ECD_00775at 828.867 kb on + strand, within ECD_00775at 828.867 kb on + strand, within ECD_00775at 828.899 kb on + strand, within ECD_00775at 828.985 kb on + strand, within ECD_00775at 829.018 kb on + strand, within ECD_00775at 829.400 kb on + strandat 829.400 kb on + strandat 829.510 kb on - strandat 829.511 kb on + strandat 829.511 kb on + strandat 829.511 kb on + strandat 829.511 kb on + strandat 829.511 kb on + strandat 829.511 kb on + strandat 829.512 kb on - strandat 829.588 kb on - strandat 829.731 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction P2_phage_0.5625_MOI
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826,269 - -0.6
826,323 + -0.4
826,672 - ECD_00774 0.38 -1.6
826,699 + ECD_00774 0.40 -2.6
827,102 + ECD_00774 0.84 +0.1
827,248 + +0.9
827,315 - +2.0
827,656 + ECD_00775 0.18 -0.6
827,677 - ECD_00775 0.19 +0.1
827,790 - ECD_00775 0.24 +1.5
827,832 + ECD_00775 0.26 +0.6
827,943 - ECD_00775 0.31 -0.5
828,242 + ECD_00775 0.45 -1.6
828,243 - ECD_00775 0.45 +2.8
828,243 - ECD_00775 0.45 -2.8
828,252 - ECD_00775 0.45 +2.0
828,252 - ECD_00775 0.45 -0.2
828,252 - ECD_00775 0.45 +0.9
828,524 + ECD_00775 0.57 -1.5
828,598 - ECD_00775 0.61 -1.3
828,598 - ECD_00775 0.61 -2.1
828,648 + ECD_00775 0.63 -0.9
828,649 - ECD_00775 0.63 -1.6
828,753 + ECD_00775 0.68 +0.2
828,754 - ECD_00775 0.68 +1.7
828,867 + ECD_00775 0.73 -0.1
828,867 + ECD_00775 0.73 -2.0
828,899 + ECD_00775 0.74 -1.4
828,985 + ECD_00775 0.78 +0.9
829,018 + ECD_00775 0.80 -0.2
829,400 + +1.4
829,400 + -1.4
829,510 - -1.5
829,511 + -0.7
829,511 + -0.9
829,511 + -1.2
829,511 + +0.1
829,511 + -2.1
829,511 + -0.5
829,512 - +1.9
829,588 - -0.1
829,731 + -0.9

Or see this region's nucleotide sequence