Strain Fitness in Escherichia coli BL21 around ECD_00680

Experiment: P2_phage_0.5625_MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00679 and ECD_00680 are separated by 389 nucleotidesECD_00680 and ECD_00681 are separated by 708 nucleotides ECD_00679: ECD_00679 - putative fimbrial-like adhesin protein, at 710,799 to 711,365 _00679 ECD_00680: ECD_00680 - citrate synthase, at 711,755 to 713,038 _00680 ECD_00681: ECD_00681 - succinate dehydrogenase, membrane subunit, binds cytochrome b556, at 713,747 to 714,136 _00681 Position (kb) 711 712 713 714Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 710.858 kb on + strand, within ECD_00679at 711.800 kb on - strandat 711.801 kb on + strandat 711.829 kb on + strandat 711.875 kb on - strandat 712.157 kb on - strand, within ECD_00680at 712.319 kb on - strand, within ECD_00680at 712.373 kb on - strand, within ECD_00680at 712.381 kb on + strand, within ECD_00680at 712.439 kb on - strand, within ECD_00680at 712.459 kb on - strand, within ECD_00680at 712.503 kb on + strand, within ECD_00680at 712.504 kb on - strand, within ECD_00680at 712.510 kb on - strand, within ECD_00680at 712.583 kb on - strand, within ECD_00680at 712.628 kb on - strand, within ECD_00680at 712.628 kb on - strand, within ECD_00680at 712.738 kb on - strand, within ECD_00680at 712.776 kb on + strand, within ECD_00680at 712.777 kb on - strand, within ECD_00680at 712.792 kb on - strand, within ECD_00680at 712.792 kb on - strand, within ECD_00680at 712.843 kb on + strand, within ECD_00680at 712.951 kb on + strandat 713.060 kb on - strandat 713.243 kb on - strandat 713.308 kb on - strandat 713.476 kb on + strandat 713.722 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction P2_phage_0.5625_MOI
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710,858 + ECD_00679 0.10 -0.4
711,800 - -2.7
711,801 + -1.0
711,829 + -0.8
711,875 - -2.5
712,157 - ECD_00680 0.31 -0.3
712,319 - ECD_00680 0.44 -1.8
712,373 - ECD_00680 0.48 -0.4
712,381 + ECD_00680 0.49 -0.8
712,439 - ECD_00680 0.53 +0.7
712,459 - ECD_00680 0.55 -1.1
712,503 + ECD_00680 0.58 -1.2
712,504 - ECD_00680 0.58 +0.9
712,510 - ECD_00680 0.59 -1.8
712,583 - ECD_00680 0.64 -2.2
712,628 - ECD_00680 0.68 -0.2
712,628 - ECD_00680 0.68 +0.1
712,738 - ECD_00680 0.77 -2.2
712,776 + ECD_00680 0.80 -0.6
712,777 - ECD_00680 0.80 +1.4
712,792 - ECD_00680 0.81 -1.8
712,792 - ECD_00680 0.81 -1.5
712,843 + ECD_00680 0.85 -2.2
712,951 + -0.9
713,060 - -0.1
713,243 - -1.0
713,308 - +4.0
713,476 + +1.5
713,722 + -1.4

Or see this region's nucleotide sequence