Strain Fitness in Escherichia coli BL21 around ECD_00673

Experiment: P2_phage_0.5625_MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00671 and ECD_00672 overlap by 7 nucleotidesECD_00672 and ECD_00673 overlap by 11 nucleotidesECD_00673 and ECD_00674 are separated by 35 nucleotidesECD_00674 and ECD_00675 overlap by 4 nucleotides ECD_00671: ECD_00671 - putative allophanate hydrolase, subunit 1, at 702,157 to 702,813 _00671 ECD_00672: ECD_00672 - putative allophanate hydrolase, subunit 2, at 702,807 to 703,739 _00672 ECD_00673: ECD_00673 - UPF0271 family protein, at 703,729 to 704,463 _00673 ECD_00674: ECD_00674 - endonuclease VIII and 5-formyluracil/5-hydroxymethyluracil DNA glycosylase, at 704,499 to 705,290 _00674 ECD_00675: ECD_00675 - regulator of aidB expression; inner membrane protein, at 705,287 to 706,333 _00675 Position (kb) 703 704 705Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 702.758 kb on + strandat 702.758 kb on + strandat 702.758 kb on + strandat 702.758 kb on + strandat 702.761 kb on + strandat 702.788 kb on + strandat 702.834 kb on + strandat 702.834 kb on + strandat 702.834 kb on + strandat 702.834 kb on + strandat 702.834 kb on + strandat 702.834 kb on + strandat 702.835 kb on - strandat 702.835 kb on - strandat 702.836 kb on + strandat 703.055 kb on + strand, within ECD_00672at 703.055 kb on + strand, within ECD_00672at 703.112 kb on + strand, within ECD_00672at 703.160 kb on + strand, within ECD_00672at 703.283 kb on + strand, within ECD_00672at 703.350 kb on + strand, within ECD_00672at 703.439 kb on - strand, within ECD_00672at 703.480 kb on + strand, within ECD_00672at 703.602 kb on - strand, within ECD_00672at 703.602 kb on - strand, within ECD_00672at 703.605 kb on + strand, within ECD_00672at 703.605 kb on + strand, within ECD_00672at 704.056 kb on + strand, within ECD_00673at 704.057 kb on - strand, within ECD_00673at 704.111 kb on - strand, within ECD_00673at 704.111 kb on - strand, within ECD_00673at 704.304 kb on - strand, within ECD_00673at 704.427 kb on + strandat 704.471 kb on + strandat 704.687 kb on - strand, within ECD_00674at 704.810 kb on + strand, within ECD_00674at 705.006 kb on + strand, within ECD_00674at 705.006 kb on + strand, within ECD_00674at 705.006 kb on + strand, within ECD_00674at 705.155 kb on + strand, within ECD_00674at 705.155 kb on + strand, within ECD_00674at 705.156 kb on - strand, within ECD_00674at 705.352 kb on + strandat 705.385 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction P2_phage_0.5625_MOI
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702,758 + +3.3
702,758 + +2.2
702,758 + -2.0
702,758 + +1.9
702,761 + +0.4
702,788 + -1.3
702,834 + -1.0
702,834 + +2.6
702,834 + +0.2
702,834 + +0.1
702,834 + +1.1
702,834 + +1.9
702,835 - +0.9
702,835 - +2.3
702,836 + +0.1
703,055 + ECD_00672 0.27 +1.2
703,055 + ECD_00672 0.27 +1.2
703,112 + ECD_00672 0.33 +1.7
703,160 + ECD_00672 0.38 -1.4
703,283 + ECD_00672 0.51 +0.1
703,350 + ECD_00672 0.58 -1.0
703,439 - ECD_00672 0.68 +2.7
703,480 + ECD_00672 0.72 -0.7
703,602 - ECD_00672 0.85 -2.0
703,602 - ECD_00672 0.85 -1.1
703,605 + ECD_00672 0.86 +1.9
703,605 + ECD_00672 0.86 +1.0
704,056 + ECD_00673 0.44 +4.4
704,057 - ECD_00673 0.45 -2.1
704,111 - ECD_00673 0.52 -0.6
704,111 - ECD_00673 0.52 -1.5
704,304 - ECD_00673 0.78 -1.5
704,427 + -2.2
704,471 + +2.3
704,687 - ECD_00674 0.24 -3.2
704,810 + ECD_00674 0.39 -1.1
705,006 + ECD_00674 0.64 +0.0
705,006 + ECD_00674 0.64 -1.6
705,006 + ECD_00674 0.64 +0.4
705,155 + ECD_00674 0.83 -1.0
705,155 + ECD_00674 0.83 -0.1
705,156 - ECD_00674 0.83 -2.5
705,352 + +0.3
705,385 + +0.4

Or see this region's nucleotide sequence