Strain Fitness in Escherichia coli BL21 around ECD_00607

Experiment: P2_phage_0.5625_MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00605 and ECD_00606 are separated by 3 nucleotidesECD_00606 and ECD_00607 are separated by 259 nucleotidesECD_00607 and ECD_00608 are separated by 23 nucleotidesECD_00608 and ECD_00609 are separated by 1 nucleotides ECD_00605: ECD_00605 - 23S rRNA m(3)Psi1915 pseudouridine methyltransferase, SAM-dependent, at 628,611 to 629,078 _00605 ECD_00606: ECD_00606 - ribosomal silencing factor, at 629,082 to 629,399 _00606 ECD_00607: ECD_00607 - putative alpha-ribazole-5'-P phosphatase, at 629,659 to 630,270 _00607 ECD_00608: ECD_00608 - nicotinic acid mononucleotide adenylyltransferase, NAD(P)-dependent, at 630,294 to 630,935 _00608 ECD_00609: ECD_00609 - DNA polymerase III, delta subunit, at 630,937 to 631,968 _00609 Position (kb) 629 630 631Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 628.704 kb on - strand, within ECD_00605at 629.108 kb on - strandat 629.256 kb on - strand, within ECD_00606at 629.256 kb on - strand, within ECD_00606at 629.256 kb on - strand, within ECD_00606at 629.256 kb on - strand, within ECD_00606at 629.256 kb on - strand, within ECD_00606at 629.256 kb on - strand, within ECD_00606at 629.256 kb on - strand, within ECD_00606at 629.274 kb on - strand, within ECD_00606at 629.445 kb on - strandat 629.445 kb on - strandat 629.816 kb on - strand, within ECD_00607at 629.836 kb on + strand, within ECD_00607at 629.865 kb on + strand, within ECD_00607at 630.048 kb on + strand, within ECD_00607at 630.119 kb on - strand, within ECD_00607at 630.146 kb on - strand, within ECD_00607at 630.153 kb on + strand, within ECD_00607at 630.154 kb on - strand, within ECD_00607at 630.215 kb on + strandat 630.302 kb on - strandat 630.953 kb on - strandat 630.988 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction P2_phage_0.5625_MOI
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628,704 - ECD_00605 0.20 +1.7
629,108 - -1.3
629,256 - ECD_00606 0.55 +0.7
629,256 - ECD_00606 0.55 +0.6
629,256 - ECD_00606 0.55 +4.5
629,256 - ECD_00606 0.55 +2.4
629,256 - ECD_00606 0.55 +2.0
629,256 - ECD_00606 0.55 -1.2
629,256 - ECD_00606 0.55 -0.4
629,274 - ECD_00606 0.60 -0.2
629,445 - -1.4
629,445 - +1.1
629,816 - ECD_00607 0.26 -1.6
629,836 + ECD_00607 0.29 -0.3
629,865 + ECD_00607 0.34 -2.0
630,048 + ECD_00607 0.64 +1.5
630,119 - ECD_00607 0.75 +1.7
630,146 - ECD_00607 0.80 -1.1
630,153 + ECD_00607 0.81 -0.6
630,154 - ECD_00607 0.81 -0.4
630,215 + +0.4
630,302 - -1.0
630,953 - +0.5
630,988 + -1.4

Or see this region's nucleotide sequence