Strain Fitness in Escherichia coli BL21 around ECD_00423

Experiment: P2_phage_0.5625_MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00421 and ECD_00422 are separated by 52 nucleotidesECD_00422 and ECD_00423 overlap by 1 nucleotidesECD_00423 and ECD_00424 are separated by 109 nucleotides ECD_00421: ECD_00421 - DNA polymerase III/DNA elongation factor III, tau and gamma subunits, at 461,138 to 463,069 _00421 ECD_00422: ECD_00422 - DNA-binding protein, putative nucleoid-associated protein, at 463,122 to 463,451 _00422 ECD_00423: ECD_00423 - gap repair protein, at 463,451 to 464,056 _00423 ECD_00424: ECD_00424 - protein refolding molecular co-chaperone Hsp90, Hsp70-dependent; heat-shock protein; ATPase, at 464,166 to 466,040 _00424 Position (kb) 463 464 465Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 463.084 kb on + strandat 463.084 kb on + strandat 463.121 kb on + strandat 463.121 kb on + strandat 463.121 kb on + strandat 463.480 kb on + strandat 463.727 kb on + strand, within ECD_00423at 463.763 kb on + strand, within ECD_00423at 463.916 kb on + strand, within ECD_00423at 463.916 kb on + strand, within ECD_00423at 463.916 kb on + strand, within ECD_00423at 463.916 kb on + strand, within ECD_00423at 463.916 kb on + strand, within ECD_00423at 463.917 kb on - strand, within ECD_00423at 463.941 kb on - strand, within ECD_00423at 464.042 kb on - strandat 464.094 kb on + strandat 464.095 kb on - strandat 464.150 kb on - strandat 464.158 kb on - strandat 464.164 kb on - strandat 464.164 kb on - strandat 464.164 kb on - strandat 464.164 kb on - strandat 464.270 kb on + strandat 464.270 kb on + strandat 464.271 kb on - strandat 464.271 kb on - strandat 464.276 kb on + strandat 464.649 kb on + strand, within ECD_00424at 464.649 kb on + strand, within ECD_00424at 464.649 kb on + strand, within ECD_00424at 464.649 kb on + strand, within ECD_00424at 464.649 kb on + strand, within ECD_00424at 464.763 kb on + strand, within ECD_00424at 464.987 kb on + strand, within ECD_00424at 464.987 kb on + strand, within ECD_00424at 464.988 kb on - strand, within ECD_00424at 465.000 kb on + strand, within ECD_00424at 465.000 kb on + strand, within ECD_00424at 465.044 kb on + strand, within ECD_00424at 465.044 kb on + strand, within ECD_00424

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Per-strain Table

Position Strand Gene LocusTag Fraction P2_phage_0.5625_MOI
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463,084 + +1.1
463,084 + -0.4
463,121 + -1.8
463,121 + -2.2
463,121 + -1.1
463,480 + -1.8
463,727 + ECD_00423 0.46 -0.6
463,763 + ECD_00423 0.51 -1.6
463,916 + ECD_00423 0.77 -2.2
463,916 + ECD_00423 0.77 -0.8
463,916 + ECD_00423 0.77 -2.9
463,916 + ECD_00423 0.77 -1.2
463,916 + ECD_00423 0.77 -2.6
463,917 - ECD_00423 0.77 -2.4
463,941 - ECD_00423 0.81 -1.3
464,042 - +0.4
464,094 + +1.6
464,095 - +2.1
464,150 - -1.5
464,158 - -1.6
464,164 - -1.8
464,164 - -1.6
464,164 - -2.2
464,164 - +2.0
464,270 + -0.1
464,270 + +2.2
464,271 - -1.5
464,271 - +1.1
464,276 + +1.0
464,649 + ECD_00424 0.26 +1.9
464,649 + ECD_00424 0.26 -0.3
464,649 + ECD_00424 0.26 -0.5
464,649 + ECD_00424 0.26 +3.4
464,649 + ECD_00424 0.26 -1.8
464,763 + ECD_00424 0.32 +0.7
464,987 + ECD_00424 0.44 +1.7
464,987 + ECD_00424 0.44 +3.1
464,988 - ECD_00424 0.44 -1.7
465,000 + ECD_00424 0.44 +0.3
465,000 + ECD_00424 0.44 -0.3
465,044 + ECD_00424 0.47 +0.6
465,044 + ECD_00424 0.47 +1.1

Or see this region's nucleotide sequence