Experiment: P2_phage_0.5625_MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECD_00365 and ECD_00366 overlap by 23 nucleotides ECD_00366 and ECD_00367 are separated by 53 nucleotides ECD_00367 and ECD_00368 are separated by 179 nucleotides
ECD_00365: ECD_00365 - thiamine monophosphate kinase, at 401,035 to 402,012
_00365
ECD_00366: ECD_00366 - phosphatidylglycerophosphatase A, at 401,990 to 402,508
_00366
ECD_00367: ECD_00367 - 2-carboxybenzaldehyde reductase, at 402,562 to 403,536
_00367
ECD_00368: ECD_00368 - 1-deoxyxylulose-5-phosphate synthase, thiamine triphosphate-binding, FAD-requiring, at 403,716 to 405,578
_00368
Position (kb)
402
403
404 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 402.043 kb on + strand, within ECD_00366 at 402.043 kb on + strand, within ECD_00366 at 402.057 kb on + strand, within ECD_00366 at 402.084 kb on + strand, within ECD_00366 at 402.137 kb on + strand, within ECD_00366 at 402.206 kb on + strand, within ECD_00366 at 402.507 kb on - strand at 402.682 kb on - strand, within ECD_00367 at 402.933 kb on - strand, within ECD_00367 at 403.099 kb on + strand, within ECD_00367 at 403.179 kb on - strand, within ECD_00367 at 403.360 kb on - strand, within ECD_00367 at 403.360 kb on - strand, within ECD_00367 at 403.563 kb on - strand at 403.611 kb on - strand at 403.615 kb on - strand at 403.615 kb on - strand at 403.615 kb on - strand at 403.665 kb on + strand at 403.666 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P2_phage_0.5625_MOI remove 402,043 + ECD_00366 0.10 -0.1 402,043 + ECD_00366 0.10 -2.7 402,057 + ECD_00366 0.13 -1.3 402,084 + ECD_00366 0.18 -0.1 402,137 + ECD_00366 0.28 -1.8 402,206 + ECD_00366 0.42 -2.3 402,507 - -1.5 402,682 - ECD_00367 0.12 -1.5 402,933 - ECD_00367 0.38 -1.3 403,099 + ECD_00367 0.55 -0.9 403,179 - ECD_00367 0.63 -2.3 403,360 - ECD_00367 0.82 -2.3 403,360 - ECD_00367 0.82 -2.1 403,563 - +0.0 403,611 - -0.5 403,615 - -2.3 403,615 - +0.1 403,615 - -1.4 403,665 + +0.3 403,666 - +2.6
Or see this region's nucleotide sequence