Strain Fitness in Escherichia coli BL21 around ECD_00323

Experiment: P2_phage_0.5625_MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00321 and ECD_00322 are separated by 87 nucleotidesECD_00322 and ECD_00323 are separated by 0 nucleotidesECD_00323 and ECD_00324 are separated by 351 nucleotides ECD_00321: ECD_00321 - hypothetical protein, at 356,137 to 359,043 _00321 ECD_00322: ECD_00322 - putative transcriptional regulator, at 359,131 to 359,754 _00322 ECD_00323: ECD_00323 - D-alanyl-D-alanine- carboxypeptidase/endopeptidase; penicillin-binding protein; weak beta-lactamase, at 359,755 to 360,912 _00323 ECD_00324: ECD_00324 - peptide antibiotic transporter, at 361,264 to 362,484 _00324 Position (kb) 359 360 361Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 358.760 kb on - strandat 358.785 kb on + strandat 358.785 kb on + strandat 358.785 kb on + strandat 358.785 kb on + strandat 358.786 kb on - strandat 358.786 kb on - strandat 358.795 kb on + strandat 358.796 kb on - strandat 358.837 kb on + strandat 358.837 kb on + strandat 358.855 kb on - strandat 358.974 kb on + strandat 358.979 kb on - strandat 358.979 kb on - strandat 359.445 kb on + strand, within ECD_00322at 359.749 kb on + strandat 359.750 kb on - strandat 359.753 kb on - strandat 359.792 kb on - strandat 359.878 kb on - strand, within ECD_00323at 359.902 kb on - strand, within ECD_00323at 359.903 kb on + strand, within ECD_00323at 359.903 kb on + strand, within ECD_00323at 359.904 kb on - strand, within ECD_00323at 359.904 kb on - strand, within ECD_00323at 359.904 kb on - strand, within ECD_00323at 359.904 kb on - strand, within ECD_00323at 359.971 kb on - strand, within ECD_00323at 359.971 kb on - strand, within ECD_00323at 359.971 kb on - strand, within ECD_00323at 359.972 kb on + strand, within ECD_00323at 359.973 kb on - strand, within ECD_00323at 359.973 kb on - strand, within ECD_00323at 359.984 kb on - strand, within ECD_00323at 359.984 kb on - strand, within ECD_00323at 360.110 kb on + strand, within ECD_00323at 360.248 kb on + strand, within ECD_00323at 360.248 kb on + strand, within ECD_00323at 360.248 kb on + strand, within ECD_00323at 360.249 kb on - strand, within ECD_00323at 360.249 kb on - strand, within ECD_00323at 360.305 kb on + strand, within ECD_00323at 360.305 kb on + strand, within ECD_00323at 360.305 kb on + strand, within ECD_00323at 360.305 kb on + strand, within ECD_00323at 360.305 kb on + strand, within ECD_00323at 360.360 kb on + strand, within ECD_00323at 360.539 kb on + strand, within ECD_00323at 360.539 kb on + strand, within ECD_00323at 360.539 kb on + strand, within ECD_00323at 360.539 kb on + strand, within ECD_00323at 360.573 kb on + strand, within ECD_00323at 360.574 kb on - strand, within ECD_00323at 360.574 kb on - strand, within ECD_00323at 360.574 kb on - strand, within ECD_00323at 360.590 kb on - strand, within ECD_00323at 360.693 kb on + strand, within ECD_00323at 360.696 kb on + strand, within ECD_00323at 360.696 kb on + strand, within ECD_00323at 360.716 kb on + strand, within ECD_00323at 360.747 kb on + strand, within ECD_00323at 360.813 kb on + strandat 360.951 kb on - strandat 360.955 kb on - strandat 360.955 kb on - strandat 360.996 kb on + strandat 360.996 kb on + strandat 360.999 kb on + strandat 361.087 kb on + strandat 361.088 kb on - strandat 361.091 kb on + strandat 361.150 kb on + strandat 361.162 kb on + strandat 361.202 kb on + strandat 361.202 kb on + strandat 361.203 kb on - strandat 361.234 kb on + strandat 361.263 kb on + strandat 361.263 kb on + strandat 361.264 kb on - strandat 361.453 kb on + strand, within ECD_00324at 361.454 kb on - strand, within ECD_00324at 361.597 kb on + strand, within ECD_00324at 361.610 kb on - strand, within ECD_00324at 361.747 kb on + strand, within ECD_00324at 361.748 kb on - strand, within ECD_00324at 361.764 kb on + strand, within ECD_00324at 361.780 kb on + strand, within ECD_00324at 361.783 kb on + strand, within ECD_00324at 361.783 kb on + strand, within ECD_00324at 361.784 kb on - strand, within ECD_00324at 361.875 kb on + strand, within ECD_00324

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Per-strain Table

Position Strand Gene LocusTag Fraction P2_phage_0.5625_MOI
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358,760 - +0.2
358,785 + +0.6
358,785 + -2.0
358,785 + +1.4
358,785 + +1.6
358,786 - -1.5
358,786 - -1.2
358,795 + +1.1
358,796 - -2.2
358,837 + -1.2
358,837 + +0.5
358,855 - +1.7
358,974 + +0.0
358,979 - -0.3
358,979 - -3.1
359,445 + ECD_00322 0.50 +0.5
359,749 + +1.2
359,750 - -0.8
359,753 - -0.6
359,792 - -0.5
359,878 - ECD_00323 0.11 -0.9
359,902 - ECD_00323 0.13 +0.9
359,903 + ECD_00323 0.13 -0.8
359,903 + ECD_00323 0.13 -2.7
359,904 - ECD_00323 0.13 -2.5
359,904 - ECD_00323 0.13 +0.5
359,904 - ECD_00323 0.13 -2.6
359,904 - ECD_00323 0.13 -2.4
359,971 - ECD_00323 0.19 +0.4
359,971 - ECD_00323 0.19 -2.9
359,971 - ECD_00323 0.19 -1.6
359,972 + ECD_00323 0.19 -1.4
359,973 - ECD_00323 0.19 +1.4
359,973 - ECD_00323 0.19 +1.8
359,984 - ECD_00323 0.20 +0.4
359,984 - ECD_00323 0.20 -2.1
360,110 + ECD_00323 0.31 -1.0
360,248 + ECD_00323 0.43 +0.8
360,248 + ECD_00323 0.43 +1.0
360,248 + ECD_00323 0.43 +1.3
360,249 - ECD_00323 0.43 +2.1
360,249 - ECD_00323 0.43 +3.0
360,305 + ECD_00323 0.47 -0.7
360,305 + ECD_00323 0.47 +1.2
360,305 + ECD_00323 0.47 -0.6
360,305 + ECD_00323 0.47 -0.9
360,305 + ECD_00323 0.47 +1.9
360,360 + ECD_00323 0.52 -0.3
360,539 + ECD_00323 0.68 -0.4
360,539 + ECD_00323 0.68 +2.9
360,539 + ECD_00323 0.68 -3.0
360,539 + ECD_00323 0.68 +0.1
360,573 + ECD_00323 0.71 -1.0
360,574 - ECD_00323 0.71 -1.4
360,574 - ECD_00323 0.71 -1.0
360,574 - ECD_00323 0.71 -3.1
360,590 - ECD_00323 0.72 -1.8
360,693 + ECD_00323 0.81 -1.8
360,696 + ECD_00323 0.81 -0.9
360,696 + ECD_00323 0.81 +0.4
360,716 + ECD_00323 0.83 -1.6
360,747 + ECD_00323 0.86 -1.0
360,813 + -1.6
360,951 - -0.9
360,955 - -1.1
360,955 - +1.6
360,996 + +0.8
360,996 + -1.9
360,999 + +1.1
361,087 + +0.3
361,088 - +2.4
361,091 + +0.5
361,150 + +1.3
361,162 + +2.1
361,202 + +2.6
361,202 + +0.8
361,203 - -2.1
361,234 + -1.7
361,263 + -1.2
361,263 + +1.7
361,264 - -2.2
361,453 + ECD_00324 0.15 -2.0
361,454 - ECD_00324 0.16 -0.6
361,597 + ECD_00324 0.27 +1.5
361,610 - ECD_00324 0.28 -1.6
361,747 + ECD_00324 0.40 -0.3
361,748 - ECD_00324 0.40 -1.9
361,764 + ECD_00324 0.41 +1.4
361,780 + ECD_00324 0.42 -0.9
361,783 + ECD_00324 0.43 +0.5
361,783 + ECD_00324 0.43 -1.4
361,784 - ECD_00324 0.43 -2.4
361,875 + ECD_00324 0.50 +0.5

Or see this region's nucleotide sequence