Experiment: P2_phage_0.5625_MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECD_00298 and ECD_00299 are separated by 122 nucleotides ECD_00299 and ECD_00300 are separated by 76 nucleotides
ECD_00298: ECD_00298 - beta-D-galactosidase, at 332,766 to 335,840
_00298
ECD_00299: ECD_00299 - lactose-inducible lac operon transcriptional repressor, at 335,963 to 337,045
_00299
ECD_00300: ECD_00300 - mhp operon transcriptional activator, at 337,122 to 337,955
_00300
Position (kb) 335
336
337
338 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 335.077 kb on - strand, within ECD_00298 at 335.190 kb on - strand, within ECD_00298 at 335.840 kb on + strand at 335.890 kb on - strand at 337.129 kb on - strand at 337.164 kb on - strand at 337.264 kb on + strand, within ECD_00300 at 337.264 kb on + strand, within ECD_00300 at 337.265 kb on - strand, within ECD_00300 at 337.265 kb on - strand, within ECD_00300 at 337.265 kb on - strand, within ECD_00300 at 337.430 kb on - strand, within ECD_00300 at 337.777 kb on + strand, within ECD_00300 at 337.777 kb on + strand, within ECD_00300 at 337.777 kb on + strand, within ECD_00300 at 337.777 kb on + strand, within ECD_00300 at 337.778 kb on - strand, within ECD_00300 at 337.778 kb on - strand, within ECD_00300 at 337.778 kb on - strand, within ECD_00300 at 337.886 kb on + strand at 337.891 kb on - strand at 337.978 kb on - strand at 338.043 kb on + strand at 338.044 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P2_phage_0.5625_MOI remove 335,077 - ECD_00298 0.75 +0.2 335,190 - ECD_00298 0.79 -0.7 335,840 + -1.1 335,890 - -1.7 337,129 - -1.2 337,164 - -2.8 337,264 + ECD_00300 0.17 +0.1 337,264 + ECD_00300 0.17 +0.7 337,265 - ECD_00300 0.17 -1.6 337,265 - ECD_00300 0.17 +4.4 337,265 - ECD_00300 0.17 -2.5 337,430 - ECD_00300 0.37 -2.0 337,777 + ECD_00300 0.79 -0.7 337,777 + ECD_00300 0.79 +1.6 337,777 + ECD_00300 0.79 -0.6 337,777 + ECD_00300 0.79 +0.0 337,778 - ECD_00300 0.79 +0.8 337,778 - ECD_00300 0.79 +1.6 337,778 - ECD_00300 0.79 -2.2 337,886 + +0.7 337,891 - +2.9 337,978 - +1.0 338,043 + +1.2 338,044 - -1.5
Or see this region's nucleotide sequence