Experiment: P2_phage_0.5625_MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECD_00187 and ECD_00188 overlap by 8 nucleotides ECD_00188 and ECD_00189 are separated by 165 nucleotides ECD_00189 and ECD_00190 overlap by 4 nucleotides ECD_00190 and ECD_00191 are separated by 13 nucleotides ECD_00191 and ECD_00192 are separated by 199 nucleotides
ECD_00187: ECD_00187 - modulator of Rho-dependent transcription termination, at 216,521 to 216,775
_00187
ECD_00188: ECD_00188 - UPF0253 family protein, at 216,768 to 216,968
_00188
ECD_00189: ECD_00189 - PDDEXK superfamily protein, at 217,134 to 217,679
_00189
ECD_00190: ECD_00190 - alternative stalled-ribosome rescue factor B; peptidyl-tRNA hydrolase, ribosome-attached, at 217,676 to 218,098
_00190
ECD_00191: ECD_00191 - lipoprotein involved with copper homeostasis and adhesion, at 218,112 to 218,822
_00191
ECD_00192: ECD_00192 - putative lipoprotein, at 219,022 to 219,846
_00192
Position (kb) 217
218
219 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 216.704 kb on + strand, within ECD_00187 at 216.705 kb on - strand, within ECD_00187 at 216.739 kb on - strand, within ECD_00187 at 216.739 kb on - strand, within ECD_00187 at 216.763 kb on - strand at 216.767 kb on - strand at 216.767 kb on - strand at 216.811 kb on + strand, within ECD_00188 at 216.812 kb on - strand, within ECD_00188 at 216.910 kb on - strand, within ECD_00188 at 216.910 kb on - strand, within ECD_00188 at 216.959 kb on - strand at 217.012 kb on + strand at 217.049 kb on + strand at 217.050 kb on - strand at 217.050 kb on - strand at 217.051 kb on + strand at 217.051 kb on + strand at 217.051 kb on + strand at 217.051 kb on + strand at 217.052 kb on - strand at 217.052 kb on - strand at 217.052 kb on - strand at 217.052 kb on - strand at 217.093 kb on - strand at 217.250 kb on + strand, within ECD_00189 at 217.251 kb on - strand, within ECD_00189 at 217.281 kb on + strand, within ECD_00189 at 217.281 kb on + strand, within ECD_00189 at 217.452 kb on + strand, within ECD_00189 at 217.452 kb on + strand, within ECD_00189 at 217.452 kb on + strand, within ECD_00189 at 217.452 kb on + strand, within ECD_00189 at 217.453 kb on - strand, within ECD_00189 at 217.453 kb on - strand, within ECD_00189 at 217.454 kb on + strand, within ECD_00189 at 217.454 kb on + strand, within ECD_00189 at 217.638 kb on - strand at 217.769 kb on - strand, within ECD_00190 at 217.826 kb on + strand, within ECD_00190 at 217.827 kb on - strand, within ECD_00190 at 217.829 kb on + strand, within ECD_00190 at 217.829 kb on + strand, within ECD_00190 at 217.968 kb on + strand, within ECD_00190 at 217.968 kb on + strand, within ECD_00190 at 218.159 kb on + strand at 218.159 kb on + strand at 218.174 kb on + strand at 218.177 kb on + strand at 218.373 kb on + strand, within ECD_00191 at 218.378 kb on + strand, within ECD_00191 at 218.508 kb on + strand, within ECD_00191 at 218.509 kb on - strand, within ECD_00191 at 218.511 kb on - strand, within ECD_00191 at 218.536 kb on + strand, within ECD_00191 at 218.536 kb on + strand, within ECD_00191 at 218.537 kb on - strand, within ECD_00191 at 218.540 kb on + strand, within ECD_00191 at 218.568 kb on + strand, within ECD_00191 at 218.574 kb on + strand, within ECD_00191 at 218.575 kb on - strand, within ECD_00191 at 218.576 kb on + strand, within ECD_00191 at 218.695 kb on + strand, within ECD_00191 at 218.704 kb on + strand, within ECD_00191 at 218.718 kb on - strand, within ECD_00191 at 218.759 kb on - strand at 218.969 kb on + strand at 218.970 kb on - strand at 218.978 kb on + strand at 218.978 kb on + strand at 218.993 kb on - strand at 219.015 kb on + strand at 219.016 kb on - strand at 219.059 kb on + strand at 219.059 kb on + strand at 219.060 kb on - strand at 219.086 kb on + strand at 219.087 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction P2_phage_0.5625_MOI remove 216,704 + ECD_00187 0.72 -1.2 216,705 - ECD_00187 0.72 +0.7 216,739 - ECD_00187 0.85 -0.0 216,739 - ECD_00187 0.85 +0.1 216,763 - -0.7 216,767 - -1.8 216,767 - +1.2 216,811 + ECD_00188 0.21 +0.9 216,812 - ECD_00188 0.22 +1.9 216,910 - ECD_00188 0.71 -0.8 216,910 - ECD_00188 0.71 +1.6 216,959 - +2.5 217,012 + -0.9 217,049 + -1.1 217,050 - -0.8 217,050 - -0.3 217,051 + -3.0 217,051 + -1.8 217,051 + -1.6 217,051 + +0.7 217,052 - +1.1 217,052 - +1.0 217,052 - +0.2 217,052 - -0.7 217,093 - -1.5 217,250 + ECD_00189 0.21 -2.2 217,251 - ECD_00189 0.21 -1.5 217,281 + ECD_00189 0.27 -3.2 217,281 + ECD_00189 0.27 +2.9 217,452 + ECD_00189 0.58 +0.9 217,452 + ECD_00189 0.58 +1.4 217,452 + ECD_00189 0.58 -1.0 217,452 + ECD_00189 0.58 +0.2 217,453 - ECD_00189 0.58 -0.8 217,453 - ECD_00189 0.58 -1.8 217,454 + ECD_00189 0.59 -2.1 217,454 + ECD_00189 0.59 -1.1 217,638 - -0.8 217,769 - ECD_00190 0.22 -0.1 217,826 + ECD_00190 0.35 -1.6 217,827 - ECD_00190 0.36 -1.0 217,829 + ECD_00190 0.36 +1.2 217,829 + ECD_00190 0.36 -2.3 217,968 + ECD_00190 0.69 -2.3 217,968 + ECD_00190 0.69 -0.2 218,159 + +2.0 218,159 + +0.4 218,174 + -2.8 218,177 + +1.3 218,373 + ECD_00191 0.37 +1.1 218,378 + ECD_00191 0.37 +0.8 218,508 + ECD_00191 0.56 +1.3 218,509 - ECD_00191 0.56 -1.8 218,511 - ECD_00191 0.56 -1.7 218,536 + ECD_00191 0.60 -3.0 218,536 + ECD_00191 0.60 +2.6 218,537 - ECD_00191 0.60 -2.1 218,540 + ECD_00191 0.60 -1.1 218,568 + ECD_00191 0.64 +1.5 218,574 + ECD_00191 0.65 -2.0 218,575 - ECD_00191 0.65 -0.4 218,576 + ECD_00191 0.65 -0.5 218,695 + ECD_00191 0.82 +1.4 218,704 + ECD_00191 0.83 +1.0 218,718 - ECD_00191 0.85 +0.1 218,759 - +0.7 218,969 + -0.6 218,970 - -0.0 218,978 + -3.0 218,978 + -2.1 218,993 - -0.7 219,015 + +1.1 219,016 - -0.7 219,059 + +0.6 219,059 + +0.7 219,060 - +1.2 219,086 + +2.1 219,087 - -2.2
Or see this region's nucleotide sequence