Experiment: P2_phage_0.5625_MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECD_00157 and ECD_00158 overlap by 8 nucleotides ECD_00158 and ECD_00159 are separated by 83 nucleotides ECD_00159 and ECD_00160 are separated by 129 nucleotides
ECD_00157: ECD_00157 - vitamin B12 ABC transporter periplasmic binding protein, at 180,505 to 181,305
_00157
ECD_00158: ECD_00158 - 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase, at 181,298 to 181,996
_00158
ECD_00159: ECD_00159 - deoxyguanosine triphosphate triphosphohydrolase, at 182,080 to 183,597
_00159
ECD_00160: ECD_00160 - serine endoprotease (protease Do), membrane-associated, at 183,727 to 185,151
_00160
Position (kb) 182
183
184 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 181.299 kb on + strand at 181.299 kb on + strand at 181.300 kb on - strand at 182.315 kb on - strand, within ECD_00159 at 182.315 kb on - strand, within ECD_00159 at 182.352 kb on + strand, within ECD_00159 at 182.474 kb on + strand, within ECD_00159 at 182.559 kb on - strand, within ECD_00159 at 182.559 kb on - strand, within ECD_00159 at 182.618 kb on + strand, within ECD_00159 at 182.618 kb on + strand, within ECD_00159 at 182.618 kb on + strand, within ECD_00159 at 182.618 kb on + strand, within ECD_00159 at 182.618 kb on + strand, within ECD_00159 at 182.619 kb on - strand, within ECD_00159 at 182.619 kb on - strand, within ECD_00159 at 182.619 kb on - strand, within ECD_00159 at 182.619 kb on - strand, within ECD_00159 at 182.661 kb on + strand, within ECD_00159 at 182.661 kb on + strand, within ECD_00159 at 182.661 kb on + strand, within ECD_00159 at 182.662 kb on - strand, within ECD_00159 at 182.662 kb on - strand, within ECD_00159 at 182.662 kb on - strand, within ECD_00159 at 182.662 kb on - strand, within ECD_00159 at 182.662 kb on - strand, within ECD_00159 at 182.762 kb on - strand, within ECD_00159 at 182.766 kb on - strand, within ECD_00159 at 182.804 kb on - strand, within ECD_00159 at 182.894 kb on - strand, within ECD_00159 at 183.142 kb on - strand, within ECD_00159 at 183.320 kb on - strand, within ECD_00159 at 183.503 kb on - strand at 183.505 kb on + strand at 183.505 kb on + strand at 183.529 kb on + strand at 183.530 kb on - strand at 183.531 kb on + strand at 183.554 kb on - strand at 183.569 kb on - strand at 183.588 kb on - strand at 183.725 kb on - strand at 183.725 kb on - strand at 183.782 kb on + strand at 183.896 kb on + strand, within ECD_00160 at 183.896 kb on + strand, within ECD_00160 at 183.896 kb on + strand, within ECD_00160 at 183.896 kb on + strand, within ECD_00160 at 183.896 kb on + strand, within ECD_00160 at 183.896 kb on + strand, within ECD_00160 at 183.919 kb on - strand, within ECD_00160 at 184.099 kb on + strand, within ECD_00160 at 184.100 kb on - strand, within ECD_00160 at 184.100 kb on - strand, within ECD_00160 at 184.100 kb on - strand, within ECD_00160 at 184.100 kb on - strand, within ECD_00160 at 184.143 kb on + strand, within ECD_00160 at 184.254 kb on + strand, within ECD_00160 at 184.254 kb on + strand, within ECD_00160 at 184.291 kb on + strand, within ECD_00160 at 184.291 kb on + strand, within ECD_00160 at 184.292 kb on - strand, within ECD_00160 at 184.527 kb on + strand, within ECD_00160
Per-strain Table
Position Strand Gene LocusTag Fraction P2_phage_0.5625_MOI remove 181,299 + -0.4 181,299 + -0.2 181,300 - +0.5 182,315 - ECD_00159 0.15 -1.0 182,315 - ECD_00159 0.15 +1.6 182,352 + ECD_00159 0.18 -1.5 182,474 + ECD_00159 0.26 -4.2 182,559 - ECD_00159 0.32 -1.4 182,559 - ECD_00159 0.32 +0.3 182,618 + ECD_00159 0.35 +0.9 182,618 + ECD_00159 0.35 +1.7 182,618 + ECD_00159 0.35 +0.7 182,618 + ECD_00159 0.35 -0.4 182,618 + ECD_00159 0.35 -2.0 182,619 - ECD_00159 0.36 -1.1 182,619 - ECD_00159 0.36 -0.9 182,619 - ECD_00159 0.36 +0.1 182,619 - ECD_00159 0.36 +0.6 182,661 + ECD_00159 0.38 -2.2 182,661 + ECD_00159 0.38 -0.7 182,661 + ECD_00159 0.38 -0.6 182,662 - ECD_00159 0.38 -1.8 182,662 - ECD_00159 0.38 -1.8 182,662 - ECD_00159 0.38 +1.0 182,662 - ECD_00159 0.38 +2.4 182,662 - ECD_00159 0.38 -2.2 182,762 - ECD_00159 0.45 -0.8 182,766 - ECD_00159 0.45 +3.6 182,804 - ECD_00159 0.48 -1.0 182,894 - ECD_00159 0.54 -0.4 183,142 - ECD_00159 0.70 -1.0 183,320 - ECD_00159 0.82 -1.2 183,503 - -2.3 183,505 + -0.5 183,505 + -1.2 183,529 + +1.2 183,530 - +0.7 183,531 + +0.5 183,554 - +1.8 183,569 - -2.5 183,588 - -0.1 183,725 - -2.5 183,725 - +2.4 183,782 + +0.5 183,896 + ECD_00160 0.12 +1.9 183,896 + ECD_00160 0.12 +0.9 183,896 + ECD_00160 0.12 -0.1 183,896 + ECD_00160 0.12 +1.1 183,896 + ECD_00160 0.12 -3.0 183,896 + ECD_00160 0.12 -0.4 183,919 - ECD_00160 0.13 +1.0 184,099 + ECD_00160 0.26 +0.0 184,100 - ECD_00160 0.26 -1.9 184,100 - ECD_00160 0.26 -0.4 184,100 - ECD_00160 0.26 -2.1 184,100 - ECD_00160 0.26 -2.6 184,143 + ECD_00160 0.29 +0.9 184,254 + ECD_00160 0.37 -2.0 184,254 + ECD_00160 0.37 -2.2 184,291 + ECD_00160 0.40 -0.4 184,291 + ECD_00160 0.40 +0.7 184,292 - ECD_00160 0.40 -2.2 184,527 + ECD_00160 0.56 -1.4
Or see this region's nucleotide sequence