Strain Fitness in Escherichia coli BL21 around ECD_00082

Experiment: P2_phage_0.5625_MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00081 and ECD_00082 are separated by 601 nucleotidesECD_00082 and ECD_00083 are separated by 1 nucleotidesECD_00083 and ECD_00084 overlap by 4 nucleotides ECD_00081: ECD_00081 - transcriptional repressor-activator for carbon metabolism, at 90,833 to 91,837 _00081 ECD_00082: ECD_00082 - RsmH methytransferase inhibitor, at 92,439 to 92,897 _00082 ECD_00083: ECD_00083 - 16S rRNA m(4)C1402 methyltransferase, SAM-dependent, at 92,899 to 93,840 _00083 ECD_00084: ECD_00084 - membrane bound cell division leucine zipper septum protein, at 93,837 to 94,202 _00084 Position (kb) 92 93Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 91.478 kb on + strand, within ECD_00081at 91.490 kb on + strand, within ECD_00081at 91.490 kb on + strand, within ECD_00081at 91.677 kb on + strand, within ECD_00081at 91.677 kb on + strand, within ECD_00081at 91.795 kb on + strandat 91.801 kb on + strandat 91.832 kb on - strandat 91.832 kb on - strandat 91.836 kb on - strandat 91.852 kb on + strandat 91.936 kb on + strandat 91.937 kb on - strandat 91.940 kb on - strandat 91.940 kb on - strandat 91.946 kb on - strandat 92.000 kb on + strandat 92.000 kb on + strandat 92.000 kb on + strandat 92.035 kb on + strandat 92.147 kb on + strandat 92.147 kb on + strandat 92.148 kb on - strandat 92.148 kb on - strandat 92.187 kb on - strandat 92.188 kb on + strandat 92.204 kb on + strandat 92.255 kb on + strandat 92.267 kb on + strandat 92.422 kb on + strandat 92.671 kb on + strand, within ECD_00082at 92.671 kb on + strand, within ECD_00082at 92.895 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction P2_phage_0.5625_MOI
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91,478 + ECD_00081 0.64 +1.1
91,490 + ECD_00081 0.65 -0.5
91,490 + ECD_00081 0.65 -2.7
91,677 + ECD_00081 0.84 +1.1
91,677 + ECD_00081 0.84 +0.5
91,795 + -0.9
91,801 + -1.6
91,832 - -0.5
91,832 - -0.8
91,836 - -2.0
91,852 + +1.9
91,936 + +1.5
91,937 - -2.3
91,940 - +2.1
91,940 - -0.1
91,946 - +0.2
92,000 + +0.5
92,000 + -0.0
92,000 + -1.7
92,035 + +1.7
92,147 + -2.5
92,147 + -2.9
92,148 - -1.2
92,148 - -0.7
92,187 - -2.9
92,188 + +0.3
92,204 + -1.7
92,255 + +0.5
92,267 + +0.5
92,422 + -1.2
92,671 + ECD_00082 0.51 +2.3
92,671 + ECD_00082 0.51 -1.4
92,895 + -2.1

Or see this region's nucleotide sequence