Strain Fitness in Escherichia coli BL21 around ECD_00047

Experiment: P2_phage_0.5625_MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00046 and ECD_00047 are separated by 50 nucleotidesECD_00047 and ECD_00048 overlap by 4 nucleotidesECD_00048 and ECD_00049 are separated by 56 nucleotides ECD_00046: ECD_00046 - putative electron transfer flavoprotein, NAD/FAD-binding domain and ETFP adenine nucleotide-binding domain-like protein, at 47,384 to 48,325 _00046 ECD_00047: ECD_00047 - putative oxidoreductase, at 48,376 to 49,662 _00047 ECD_00048: ECD_00048 - putative 4Fe-4S ferredoxin-type protein, at 49,659 to 49,946 _00048 ECD_00049: ECD_00049 - putative MFS sugar transporter; membrane protein, at 50,003 to 51,334 _00049 Position (kb) 48 49 50Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 47.384 kb on - strandat 47.547 kb on + strand, within ECD_00046at 47.730 kb on + strand, within ECD_00046at 47.731 kb on - strand, within ECD_00046at 47.766 kb on - strand, within ECD_00046at 48.045 kb on + strand, within ECD_00046at 48.111 kb on - strand, within ECD_00046at 48.227 kb on - strand, within ECD_00046at 48.227 kb on - strand, within ECD_00046at 48.232 kb on + strandat 48.376 kb on - strandat 48.470 kb on + strandat 48.471 kb on - strandat 48.471 kb on - strandat 48.471 kb on - strandat 48.526 kb on + strand, within ECD_00047at 48.549 kb on + strand, within ECD_00047at 48.579 kb on - strand, within ECD_00047at 48.683 kb on - strand, within ECD_00047at 48.947 kb on + strand, within ECD_00047at 49.056 kb on + strand, within ECD_00047at 49.056 kb on + strand, within ECD_00047at 49.056 kb on + strand, within ECD_00047at 49.056 kb on + strand, within ECD_00047at 49.297 kb on - strand, within ECD_00047at 49.297 kb on - strand, within ECD_00047at 49.297 kb on - strand, within ECD_00047at 49.458 kb on + strand, within ECD_00047at 49.458 kb on + strand, within ECD_00047at 49.458 kb on + strand, within ECD_00047at 49.458 kb on + strand, within ECD_00047at 49.458 kb on + strand, within ECD_00047at 49.458 kb on + strand, within ECD_00047at 49.459 kb on - strand, within ECD_00047at 49.459 kb on - strand, within ECD_00047at 49.459 kb on - strand, within ECD_00047at 49.459 kb on - strand, within ECD_00047at 49.800 kb on + strand, within ECD_00048at 49.800 kb on + strand, within ECD_00048at 49.801 kb on - strand, within ECD_00048at 49.801 kb on - strand, within ECD_00048at 49.958 kb on + strandat 49.958 kb on + strandat 50.096 kb on + strandat 50.097 kb on - strandat 50.100 kb on + strandat 50.101 kb on - strandat 50.101 kb on - strandat 50.107 kb on - strandat 50.228 kb on - strand, within ECD_00049at 50.347 kb on + strand, within ECD_00049at 50.456 kb on + strand, within ECD_00049at 50.457 kb on - strand, within ECD_00049at 50.457 kb on - strand, within ECD_00049at 50.643 kb on + strand, within ECD_00049at 50.644 kb on - strand, within ECD_00049

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Per-strain Table

Position Strand Gene LocusTag Fraction P2_phage_0.5625_MOI
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47,384 - -0.1
47,547 + ECD_00046 0.17 -0.9
47,730 + ECD_00046 0.37 +1.4
47,731 - ECD_00046 0.37 +1.4
47,766 - ECD_00046 0.41 +1.5
48,045 + ECD_00046 0.70 -0.3
48,111 - ECD_00046 0.77 -1.9
48,227 - ECD_00046 0.89 +0.4
48,227 - ECD_00046 0.89 +0.2
48,232 + -1.1
48,376 - +1.2
48,470 + -0.1
48,471 - +1.9
48,471 - -0.6
48,471 - -0.2
48,526 + ECD_00047 0.12 +2.7
48,549 + ECD_00047 0.13 +0.8
48,579 - ECD_00047 0.16 +0.4
48,683 - ECD_00047 0.24 -0.3
48,947 + ECD_00047 0.44 +1.2
49,056 + ECD_00047 0.53 +1.9
49,056 + ECD_00047 0.53 -0.1
49,056 + ECD_00047 0.53 +1.9
49,056 + ECD_00047 0.53 -0.6
49,297 - ECD_00047 0.72 -1.9
49,297 - ECD_00047 0.72 +1.4
49,297 - ECD_00047 0.72 +2.9
49,458 + ECD_00047 0.84 -2.9
49,458 + ECD_00047 0.84 -3.4
49,458 + ECD_00047 0.84 +4.2
49,458 + ECD_00047 0.84 -1.7
49,458 + ECD_00047 0.84 +0.9
49,458 + ECD_00047 0.84 -0.0
49,459 - ECD_00047 0.84 -0.3
49,459 - ECD_00047 0.84 -1.8
49,459 - ECD_00047 0.84 +1.4
49,459 - ECD_00047 0.84 +2.1
49,800 + ECD_00048 0.49 +1.3
49,800 + ECD_00048 0.49 -1.7
49,801 - ECD_00048 0.49 +0.6
49,801 - ECD_00048 0.49 -2.7
49,958 + +0.2
49,958 + -1.2
50,096 + +1.2
50,097 - -1.1
50,100 + -1.7
50,101 - -2.0
50,101 - +0.8
50,107 - -2.4
50,228 - ECD_00049 0.17 -1.6
50,347 + ECD_00049 0.26 -0.9
50,456 + ECD_00049 0.34 -3.2
50,457 - ECD_00049 0.34 -1.9
50,457 - ECD_00049 0.34 +1.1
50,643 + ECD_00049 0.48 -1.0
50,644 - ECD_00049 0.48 +3.0

Or see this region's nucleotide sequence