Strain Fitness in Pseudomonas fluorescens GW456-L13 around PfGW456L13_120

Experiment: D-Alanine (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPfGW456L13_117 and PfGW456L13_118 are separated by 128 nucleotidesPfGW456L13_118 and PfGW456L13_119 are separated by 13 nucleotidesPfGW456L13_119 and PfGW456L13_120 are separated by 352 nucleotidesPfGW456L13_120 and PfGW456L13_121 overlap by 4 nucleotidesPfGW456L13_121 and PfGW456L13_122 overlap by 4 nucleotides PfGW456L13_117 - Oxidoreductase, short chain dehydrogenase/reductase family, at 134,357 to 135,118 _117 PfGW456L13_118 - hypothetical protein, at 135,247 to 135,402 _118 PfGW456L13_119 - hypothetical protein, at 135,416 to 135,604 _119 PfGW456L13_120 - Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1), at 135,957 to 136,673 _120 PfGW456L13_121 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 136,670 to 137,542 _121 PfGW456L13_122 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 137,539 to 138,822 _122 Position (kb) 135 136 137Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 135.100 kb on + strandat 135.100 kb on + strandat 135.100 kb on + strandat 135.100 kb on + strandat 135.100 kb on + strandat 135.100 kb on + strandat 135.100 kb on + strandat 135.263 kb on - strand, within PfGW456L13_118at 135.263 kb on - strand, within PfGW456L13_118at 135.263 kb on - strand, within PfGW456L13_118at 135.628 kb on + strandat 135.629 kb on - strandat 135.629 kb on - strandat 135.715 kb on - strandat 135.715 kb on - strandat 135.715 kb on - strandat 135.729 kb on + strandat 135.730 kb on - strandat 135.730 kb on - strandat 135.897 kb on + strandat 135.898 kb on - strandat 135.898 kb on - strandat 135.898 kb on - strandat 135.898 kb on - strandat 135.944 kb on + strandat 135.945 kb on - strandat 135.980 kb on + strandat 136.051 kb on + strand, within PfGW456L13_120at 136.051 kb on + strand, within PfGW456L13_120at 136.051 kb on + strand, within PfGW456L13_120at 136.051 kb on + strand, within PfGW456L13_120at 136.052 kb on - strand, within PfGW456L13_120at 136.339 kb on + strand, within PfGW456L13_120at 136.340 kb on - strand, within PfGW456L13_120at 136.340 kb on - strand, within PfGW456L13_120at 136.340 kb on - strand, within PfGW456L13_120at 136.359 kb on - strand, within PfGW456L13_120at 136.359 kb on - strand, within PfGW456L13_120at 136.388 kb on + strand, within PfGW456L13_120at 136.447 kb on + strand, within PfGW456L13_120at 136.447 kb on + strand, within PfGW456L13_120at 136.448 kb on - strand, within PfGW456L13_120at 136.448 kb on - strand, within PfGW456L13_120at 136.448 kb on - strand, within PfGW456L13_120at 136.448 kb on - strand, within PfGW456L13_120at 136.448 kb on - strand, within PfGW456L13_120at 136.610 kb on + strandat 136.611 kb on - strandat 136.611 kb on - strandat 136.611 kb on - strandat 136.667 kb on + strandat 136.667 kb on + strandat 136.668 kb on - strandat 136.673 kb on + strandat 136.674 kb on - strandat 136.674 kb on - strandat 136.674 kb on - strandat 136.701 kb on + strandat 136.702 kb on - strandat 136.795 kb on + strand, within PfGW456L13_121at 136.795 kb on + strand, within PfGW456L13_121at 136.796 kb on - strand, within PfGW456L13_121at 136.796 kb on - strand, within PfGW456L13_121at 136.796 kb on - strand, within PfGW456L13_121at 136.796 kb on - strand, within PfGW456L13_121at 136.801 kb on + strand, within PfGW456L13_121at 136.801 kb on + strand, within PfGW456L13_121at 136.802 kb on - strand, within PfGW456L13_121at 136.986 kb on + strand, within PfGW456L13_121at 137.096 kb on - strand, within PfGW456L13_121at 137.250 kb on + strand, within PfGW456L13_121at 137.250 kb on + strand, within PfGW456L13_121at 137.380 kb on - strand, within PfGW456L13_121at 137.380 kb on - strand, within PfGW456L13_121at 137.380 kb on - strand, within PfGW456L13_121at 137.380 kb on - strand, within PfGW456L13_121at 137.528 kb on + strandat 137.529 kb on - strandat 137.549 kb on - strandat 137.557 kb on - strandat 137.574 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Alanine (C)
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135,100 + +2.6
135,100 + +0.0
135,100 + -0.7
135,100 + +0.2
135,100 + +0.5
135,100 + +1.2
135,100 + +0.5
135,263 - PfGW456L13_118 0.10 -0.8
135,263 - PfGW456L13_118 0.10 +1.9
135,263 - PfGW456L13_118 0.10 -0.5
135,628 + +0.5
135,629 - -0.9
135,629 - +1.4
135,715 - -1.8
135,715 - -0.6
135,715 - +0.1
135,729 + -0.8
135,730 - +0.1
135,730 - +0.1
135,897 + +0.5
135,898 - +0.4
135,898 - -0.3
135,898 - +0.9
135,898 - +0.6
135,944 + +0.4
135,945 - -1.5
135,980 + -0.4
136,051 + PfGW456L13_120 0.13 -1.3
136,051 + PfGW456L13_120 0.13 -0.5
136,051 + PfGW456L13_120 0.13 -1.3
136,051 + PfGW456L13_120 0.13 -1.5
136,052 - PfGW456L13_120 0.13 -3.6
136,339 + PfGW456L13_120 0.53 -1.8
136,340 - PfGW456L13_120 0.53 -3.4
136,340 - PfGW456L13_120 0.53 -1.1
136,340 - PfGW456L13_120 0.53 -2.5
136,359 - PfGW456L13_120 0.56 +0.0
136,359 - PfGW456L13_120 0.56 -2.4
136,388 + PfGW456L13_120 0.60 -1.1
136,447 + PfGW456L13_120 0.68 -0.7
136,447 + PfGW456L13_120 0.68 -3.0
136,448 - PfGW456L13_120 0.68 -0.6
136,448 - PfGW456L13_120 0.68 -1.0
136,448 - PfGW456L13_120 0.68 -0.9
136,448 - PfGW456L13_120 0.68 -2.3
136,448 - PfGW456L13_120 0.68 -1.3
136,610 + -1.1
136,611 - -1.2
136,611 - -2.2
136,611 - -1.3
136,667 + -0.4
136,667 + -1.0
136,668 - -0.7
136,673 + +0.0
136,674 - +0.0
136,674 - -1.7
136,674 - -0.8
136,701 + -2.4
136,702 - +1.6
136,795 + PfGW456L13_121 0.14 +1.9
136,795 + PfGW456L13_121 0.14 -1.8
136,796 - PfGW456L13_121 0.14 -0.7
136,796 - PfGW456L13_121 0.14 -1.4
136,796 - PfGW456L13_121 0.14 -1.2
136,796 - PfGW456L13_121 0.14 -2.4
136,801 + PfGW456L13_121 0.15 -1.2
136,801 + PfGW456L13_121 0.15 -1.4
136,802 - PfGW456L13_121 0.15 -0.5
136,986 + PfGW456L13_121 0.36 -2.7
137,096 - PfGW456L13_121 0.49 -1.1
137,250 + PfGW456L13_121 0.66 -1.5
137,250 + PfGW456L13_121 0.66 -1.8
137,380 - PfGW456L13_121 0.81 -1.2
137,380 - PfGW456L13_121 0.81 +0.2
137,380 - PfGW456L13_121 0.81 -2.7
137,380 - PfGW456L13_121 0.81 -0.8
137,528 + -1.3
137,529 - -0.6
137,549 - -3.5
137,557 - -1.7
137,574 + +0.0

Or see this region's nucleotide sequence