Experiment: syringaldehyde 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt afuB and insB-2 are separated by 16 nucleotides insB-2 and insA-2 overlap by 82 nucleotides insA-2 and insX are separated by 238 nucleotides insX and yagB are separated by 64 nucleotides yagB and yagA are separated by 93 nucleotides
b0263: afuB - CP4-6 prophage; predicted ferric trasnporter subunit (RefSeq), at 278,038 to 278,385
afuB
b0264: insB-2 - CP4-6 prophage; IS1 transposase InsAB' (NCBI), at 278,402 to 278,905
insB-2
b0265: insA-2 - CP4-6 prophage; IS1 repressor protein InsA (NCBI), at 278,824 to 279,099
insA-2
b4505: insX - predicted IS protein (RefSeq), at 279,338 to 279,586
insX
b0266: yagB - orf, hypothetical protein (VIMSS), at 279,651 to 279,959
yagB
b0267: yagA - CP4-6 prophage; predicted DNA-binding transcriptional regulator (NCBI), at 280,053 to 281,207
yagA
Position (kb) 279
280 Strain fitness (log2 ratio) -2
-1
0
1
2 at 278.350 kb on + strand, within afuB at 279.472 kb on - strand, within insX at 279.476 kb on - strand, within insX at 279.573 kb on + strand at 279.581 kb on - strand at 279.581 kb on - strand at 279.584 kb on + strand at 279.584 kb on + strand at 279.624 kb on - strand at 279.624 kb on - strand at 279.714 kb on + strand, within yagB at 279.719 kb on + strand, within yagB at 279.719 kb on + strand, within yagB at 279.719 kb on + strand, within yagB at 279.721 kb on + strand, within yagB at 279.721 kb on + strand, within yagB at 279.727 kb on - strand, within yagB at 279.729 kb on - strand, within yagB at 279.729 kb on - strand, within yagB at 279.747 kb on - strand, within yagB at 279.757 kb on - strand, within yagB at 279.824 kb on - strand, within yagB at 279.829 kb on - strand, within yagB at 279.857 kb on - strand, within yagB at 279.882 kb on + strand, within yagB at 279.882 kb on + strand, within yagB at 279.890 kb on - strand, within yagB at 279.890 kb on - strand, within yagB at 279.909 kb on + strand, within yagB at 279.936 kb on + strand at 280.030 kb on + strand at 280.060 kb on + strand at 280.106 kb on + strand at 280.106 kb on + strand at 280.122 kb on - strand at 280.122 kb on - strand at 280.127 kb on + strand at 280.172 kb on - strand, within yagA at 280.182 kb on - strand, within yagA at 280.184 kb on + strand, within yagA at 280.184 kb on + strand, within yagA at 280.288 kb on + strand, within yagA at 280.288 kb on + strand, within yagA at 280.362 kb on - strand, within yagA at 280.362 kb on - strand, within yagA at 280.370 kb on - strand, within yagA at 280.390 kb on - strand, within yagA at 280.395 kb on + strand, within yagA at 280.395 kb on - strand, within yagA at 280.395 kb on - strand, within yagA at 280.398 kb on - strand, within yagA at 280.581 kb on + strand, within yagA
Per-strain Table
Position Strand Gene LocusTag Fraction syringaldehyde 5 mM remove 278,350 + afuB b0263 0.90 +0.1 279,472 - insX b4505 0.54 -1.4 279,476 - insX b4505 0.55 -1.4 279,573 + -0.5 279,581 - +0.6 279,581 - +0.5 279,584 + -1.1 279,584 + +0.6 279,624 - +0.6 279,624 - -0.3 279,714 + yagB b0266 0.20 +0.2 279,719 + yagB b0266 0.22 -0.4 279,719 + yagB b0266 0.22 -2.1 279,719 + yagB b0266 0.22 +0.4 279,721 + yagB b0266 0.23 +0.5 279,721 + yagB b0266 0.23 -1.1 279,727 - yagB b0266 0.25 +0.9 279,729 - yagB b0266 0.25 +0.1 279,729 - yagB b0266 0.25 -0.1 279,747 - yagB b0266 0.31 -1.4 279,757 - yagB b0266 0.34 -0.5 279,824 - yagB b0266 0.56 -0.3 279,829 - yagB b0266 0.58 +0.9 279,857 - yagB b0266 0.67 -1.1 279,882 + yagB b0266 0.75 -0.5 279,882 + yagB b0266 0.75 -0.3 279,890 - yagB b0266 0.77 +0.9 279,890 - yagB b0266 0.77 -0.1 279,909 + yagB b0266 0.83 -0.3 279,936 + +0.9 280,030 + +0.2 280,060 + -0.8 280,106 + +1.1 280,106 + +0.9 280,122 - +0.0 280,122 - -0.5 280,127 + +0.5 280,172 - yagA b0267 0.10 -0.1 280,182 - yagA b0267 0.11 +0.4 280,184 + yagA b0267 0.11 +1.2 280,184 + yagA b0267 0.11 -0.6 280,288 + yagA b0267 0.20 +0.9 280,288 + yagA b0267 0.20 -1.7 280,362 - yagA b0267 0.27 -1.2 280,362 - yagA b0267 0.27 -0.8 280,370 - yagA b0267 0.27 -0.7 280,390 - yagA b0267 0.29 +0.8 280,395 + yagA b0267 0.30 +1.0 280,395 - yagA b0267 0.30 -1.4 280,395 - yagA b0267 0.30 +2.5 280,398 - yagA b0267 0.30 +1.5 280,581 + yagA b0267 0.46 -1.2
Or see this region's nucleotide sequence