Strain Fitness in Escherichia coli BW25113 around b0186

Experiment: syringaldehyde 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntaccA and ldcC are separated by 98 nucleotidesldcC and yaeR are separated by 56 nucleotidesyaeR and tilS are separated by 64 nucleotides b0185: accA - acetyl-CoA carboxylase subunit alpha (NCBI), at 208,621 to 209,580 accA b0186: ldcC - lysine decarboxylase 2, constitutive (NCBI), at 209,679 to 211,820 ldcC b0187: yaeR - orf, hypothetical protein (VIMSS), at 211,877 to 212,266 yaeR b0188: tilS - tRNA(Ile)-lysidine synthetase (NCBI), at 212,331 to 213,629 tilS Position (kb) 209 210 211 212Strain fitness (log2 ratio) -1 0 1 2at 209.655 kb on - strandat 209.826 kb on - strandat 209.832 kb on - strandat 209.856 kb on + strandat 209.856 kb on + strandat 209.864 kb on - strandat 209.930 kb on - strand, within ldcCat 209.944 kb on - strand, within ldcCat 209.960 kb on + strand, within ldcCat 209.960 kb on + strand, within ldcCat 209.961 kb on - strand, within ldcCat 209.994 kb on + strand, within ldcCat 209.997 kb on - strand, within ldcCat 210.032 kb on + strand, within ldcCat 210.098 kb on - strand, within ldcCat 210.202 kb on + strand, within ldcCat 210.251 kb on + strand, within ldcCat 210.375 kb on + strand, within ldcCat 210.375 kb on + strand, within ldcCat 210.398 kb on - strand, within ldcCat 210.425 kb on - strand, within ldcCat 210.440 kb on + strand, within ldcCat 210.458 kb on - strand, within ldcCat 210.549 kb on + strand, within ldcCat 210.557 kb on - strand, within ldcCat 210.612 kb on + strand, within ldcCat 210.612 kb on + strand, within ldcCat 210.614 kb on + strand, within ldcCat 210.619 kb on - strand, within ldcCat 210.620 kb on - strand, within ldcCat 210.626 kb on - strand, within ldcCat 210.708 kb on - strand, within ldcCat 210.718 kb on - strand, within ldcCat 210.801 kb on - strand, within ldcCat 210.837 kb on - strand, within ldcCat 210.860 kb on - strand, within ldcCat 210.860 kb on - strand, within ldcCat 210.861 kb on + strand, within ldcCat 210.863 kb on - strand, within ldcCat 210.866 kb on + strand, within ldcCat 210.874 kb on - strand, within ldcCat 210.887 kb on + strand, within ldcCat 210.887 kb on + strand, within ldcCat 210.958 kb on + strand, within ldcCat 211.000 kb on - strand, within ldcCat 211.190 kb on + strand, within ldcCat 211.246 kb on + strand, within ldcCat 211.246 kb on + strand, within ldcCat 211.267 kb on + strand, within ldcCat 211.402 kb on - strand, within ldcCat 211.445 kb on + strand, within ldcCat 211.494 kb on + strand, within ldcCat 211.611 kb on - strandat 211.618 kb on + strandat 211.767 kb on + strandat 211.775 kb on - strandat 211.860 kb on + strandat 211.953 kb on + strand, within yaeRat 212.075 kb on + strand, within yaeRat 212.100 kb on + strand, within yaeRat 212.129 kb on - strand, within yaeRat 212.165 kb on + strand, within yaeRat 212.193 kb on + strand, within yaeRat 212.193 kb on + strand, within yaeRat 212.229 kb on - strandat 212.252 kb on + strandat 212.252 kb on + strandat 212.291 kb on + strandat 212.291 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction syringaldehyde 5 mM
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209,655 - +0.3
209,826 - -0.3
209,832 - +1.2
209,856 + +2.5
209,856 + +0.6
209,864 - -0.3
209,930 - ldcC b0186 0.12 -0.1
209,944 - ldcC b0186 0.12 +0.6
209,960 + ldcC b0186 0.13 +0.1
209,960 + ldcC b0186 0.13 -0.3
209,961 - ldcC b0186 0.13 -0.2
209,994 + ldcC b0186 0.15 -0.2
209,997 - ldcC b0186 0.15 -0.5
210,032 + ldcC b0186 0.16 +2.4
210,098 - ldcC b0186 0.20 +0.5
210,202 + ldcC b0186 0.24 +1.4
210,251 + ldcC b0186 0.27 -0.4
210,375 + ldcC b0186 0.32 +0.5
210,375 + ldcC b0186 0.32 +0.5
210,398 - ldcC b0186 0.34 -0.2
210,425 - ldcC b0186 0.35 +1.5
210,440 + ldcC b0186 0.36 +0.3
210,458 - ldcC b0186 0.36 -0.4
210,549 + ldcC b0186 0.41 +0.2
210,557 - ldcC b0186 0.41 +0.7
210,612 + ldcC b0186 0.44 +0.8
210,612 + ldcC b0186 0.44 -0.2
210,614 + ldcC b0186 0.44 -1.5
210,619 - ldcC b0186 0.44 +0.1
210,620 - ldcC b0186 0.44 +0.8
210,626 - ldcC b0186 0.44 +1.3
210,708 - ldcC b0186 0.48 +0.1
210,718 - ldcC b0186 0.49 +0.2
210,801 - ldcC b0186 0.52 +0.2
210,837 - ldcC b0186 0.54 +1.4
210,860 - ldcC b0186 0.55 +0.1
210,860 - ldcC b0186 0.55 +0.2
210,861 + ldcC b0186 0.55 +2.1
210,863 - ldcC b0186 0.55 +1.2
210,866 + ldcC b0186 0.55 +0.8
210,874 - ldcC b0186 0.56 +0.5
210,887 + ldcC b0186 0.56 +0.2
210,887 + ldcC b0186 0.56 +0.2
210,958 + ldcC b0186 0.60 +0.4
211,000 - ldcC b0186 0.62 +0.9
211,190 + ldcC b0186 0.71 +0.3
211,246 + ldcC b0186 0.73 -0.6
211,246 + ldcC b0186 0.73 +1.2
211,267 + ldcC b0186 0.74 +0.5
211,402 - ldcC b0186 0.80 -0.1
211,445 + ldcC b0186 0.82 +1.8
211,494 + ldcC b0186 0.85 +0.1
211,611 - +0.4
211,618 + +2.7
211,767 + -0.5
211,775 - +0.4
211,860 + +1.5
211,953 + yaeR b0187 0.19 -0.3
212,075 + yaeR b0187 0.51 +0.6
212,100 + yaeR b0187 0.57 +1.1
212,129 - yaeR b0187 0.65 -0.1
212,165 + yaeR b0187 0.74 -1.3
212,193 + yaeR b0187 0.81 +1.2
212,193 + yaeR b0187 0.81 -1.5
212,229 - +1.9
212,252 + +0.2
212,252 + -1.3
212,291 + +1.2
212,291 + -0.2

Or see this region's nucleotide sequence