Experiment: Vanillin 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wcaL and wcaK overlap by 4 nucleotides wcaK and wzxC are separated by 275 nucleotides
b2044: wcaL - predicted glycosyl transferase (NCBI), at 2,113,931 to 2,115,151
wcaL
b2045: wcaK - predicted pyruvyl transferase (NCBI), at 2,115,148 to 2,116,428
wcaK
b2046: wzxC - colanic acid exporter (NCBI), at 2,116,704 to 2,118,182
wzxC
Position (kb)
2115
2116
2117 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2114.251 kb on - strand, within wcaL at 2114.539 kb on - strand, within wcaL at 2114.789 kb on - strand, within wcaL at 2114.874 kb on - strand, within wcaL at 2115.177 kb on - strand at 2115.320 kb on + strand, within wcaK at 2115.414 kb on - strand, within wcaK at 2115.426 kb on - strand, within wcaK at 2115.426 kb on - strand, within wcaK at 2115.441 kb on + strand, within wcaK at 2115.507 kb on - strand, within wcaK at 2115.528 kb on + strand, within wcaK at 2115.548 kb on + strand, within wcaK at 2115.584 kb on + strand, within wcaK at 2115.585 kb on - strand, within wcaK at 2115.603 kb on - strand, within wcaK at 2115.651 kb on + strand, within wcaK at 2115.660 kb on + strand, within wcaK at 2115.708 kb on + strand, within wcaK at 2115.716 kb on - strand, within wcaK at 2115.754 kb on - strand, within wcaK at 2115.790 kb on + strand, within wcaK at 2115.941 kb on + strand, within wcaK at 2115.941 kb on + strand, within wcaK at 2115.949 kb on - strand, within wcaK at 2115.959 kb on - strand, within wcaK at 2115.959 kb on - strand, within wcaK at 2115.968 kb on + strand, within wcaK at 2115.968 kb on + strand, within wcaK at 2115.976 kb on - strand, within wcaK at 2115.995 kb on + strand, within wcaK at 2116.108 kb on + strand, within wcaK at 2116.108 kb on + strand, within wcaK at 2116.413 kb on + strand at 2116.513 kb on - strand at 2116.513 kb on - strand at 2116.566 kb on - strand at 2116.566 kb on - strand at 2116.604 kb on - strand at 2116.868 kb on + strand, within wzxC at 2116.868 kb on + strand, within wzxC at 2116.880 kb on + strand, within wzxC at 2116.888 kb on - strand, within wzxC at 2116.888 kb on - strand, within wzxC at 2116.925 kb on + strand, within wzxC at 2117.172 kb on - strand, within wzxC at 2117.175 kb on + strand, within wzxC at 2117.196 kb on - strand, within wzxC at 2117.246 kb on + strand, within wzxC at 2117.262 kb on - strand, within wzxC at 2117.262 kb on - strand, within wzxC at 2117.272 kb on + strand, within wzxC at 2117.301 kb on + strand, within wzxC
Per-strain Table
Position Strand Gene LocusTag Fraction Vanillin 5 mM remove 2,114,251 - wcaL b2044 0.26 -0.1 2,114,539 - wcaL b2044 0.50 +0.2 2,114,789 - wcaL b2044 0.70 -0.6 2,114,874 - wcaL b2044 0.77 +2.0 2,115,177 - +0.9 2,115,320 + wcaK b2045 0.13 -0.2 2,115,414 - wcaK b2045 0.21 -0.0 2,115,426 - wcaK b2045 0.22 -0.3 2,115,426 - wcaK b2045 0.22 +1.7 2,115,441 + wcaK b2045 0.23 -0.3 2,115,507 - wcaK b2045 0.28 -0.8 2,115,528 + wcaK b2045 0.30 -0.3 2,115,548 + wcaK b2045 0.31 +0.7 2,115,584 + wcaK b2045 0.34 +0.3 2,115,585 - wcaK b2045 0.34 +0.3 2,115,603 - wcaK b2045 0.36 -0.7 2,115,651 + wcaK b2045 0.39 +0.9 2,115,660 + wcaK b2045 0.40 -0.9 2,115,708 + wcaK b2045 0.44 -1.1 2,115,716 - wcaK b2045 0.44 -0.1 2,115,754 - wcaK b2045 0.47 -0.9 2,115,790 + wcaK b2045 0.50 -0.0 2,115,941 + wcaK b2045 0.62 +0.1 2,115,941 + wcaK b2045 0.62 +0.7 2,115,949 - wcaK b2045 0.63 -1.2 2,115,959 - wcaK b2045 0.63 +0.2 2,115,959 - wcaK b2045 0.63 -0.0 2,115,968 + wcaK b2045 0.64 -0.9 2,115,968 + wcaK b2045 0.64 -0.2 2,115,976 - wcaK b2045 0.65 +1.4 2,115,995 + wcaK b2045 0.66 +0.2 2,116,108 + wcaK b2045 0.75 -1.9 2,116,108 + wcaK b2045 0.75 -0.7 2,116,413 + -0.3 2,116,513 - +0.4 2,116,513 - -0.0 2,116,566 - +2.5 2,116,566 - -0.7 2,116,604 - -0.3 2,116,868 + wzxC b2046 0.11 -1.9 2,116,868 + wzxC b2046 0.11 +0.7 2,116,880 + wzxC b2046 0.12 -0.8 2,116,888 - wzxC b2046 0.12 -0.6 2,116,888 - wzxC b2046 0.12 -0.4 2,116,925 + wzxC b2046 0.15 +0.3 2,117,172 - wzxC b2046 0.32 +0.3 2,117,175 + wzxC b2046 0.32 +3.5 2,117,196 - wzxC b2046 0.33 +0.5 2,117,246 + wzxC b2046 0.37 -0.0 2,117,262 - wzxC b2046 0.38 +0.7 2,117,262 - wzxC b2046 0.38 +0.7 2,117,272 + wzxC b2046 0.38 +0.4 2,117,301 + wzxC b2046 0.40 -0.4
Or see this region's nucleotide sequence