Experiment: Vanillin 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hofB and ppdD are separated by 9 nucleotides ppdD and nadC are separated by 202 nucleotides
b0107: hofB - conserved protein with nucleoside triphosphate hydrolase domain (NCBI), at 115,714 to 117,099
hofB
b0108: ppdD - predicted major pilin subunit (NCBI), at 117,109 to 117,549
ppdD
b0109: nadC - nicotinate-nucleotide pyrophosphorylase (NCBI), at 117,752 to 118,645
nadC
Position (kb) 117
118 Strain fitness (log2 ratio) -2
-1
0
1 at 116.149 kb on - strand, within hofB at 116.335 kb on + strand, within hofB at 116.399 kb on + strand, within hofB at 116.430 kb on - strand, within hofB at 116.430 kb on - strand, within hofB at 116.471 kb on - strand, within hofB at 116.760 kb on - strand, within hofB at 116.777 kb on + strand, within hofB at 116.777 kb on + strand, within hofB at 116.872 kb on + strand, within hofB at 116.882 kb on + strand, within hofB at 116.957 kb on - strand, within hofB at 116.964 kb on - strand at 117.046 kb on + strand at 117.056 kb on + strand at 117.069 kb on + strand at 117.075 kb on + strand at 117.075 kb on + strand at 117.075 kb on + strand at 117.081 kb on - strand at 117.081 kb on - strand at 117.084 kb on + strand at 117.087 kb on + strand at 117.227 kb on - strand, within ppdD at 117.281 kb on + strand, within ppdD at 117.281 kb on + strand, within ppdD at 117.293 kb on - strand, within ppdD at 117.302 kb on + strand, within ppdD at 117.302 kb on + strand, within ppdD at 117.385 kb on + strand, within ppdD at 117.387 kb on + strand, within ppdD at 117.508 kb on - strand at 117.508 kb on - strand at 117.511 kb on - strand at 117.511 kb on - strand at 117.525 kb on - strand at 117.525 kb on - strand at 117.603 kb on + strand at 117.603 kb on + strand at 117.839 kb on + strand at 117.856 kb on - strand, within nadC at 117.856 kb on - strand, within nadC at 117.879 kb on - strand, within nadC at 117.879 kb on - strand, within nadC at 117.881 kb on - strand, within nadC at 117.922 kb on - strand, within nadC at 117.922 kb on - strand, within nadC at 118.107 kb on - strand, within nadC at 118.201 kb on + strand, within nadC at 118.230 kb on + strand, within nadC at 118.230 kb on + strand, within nadC at 118.296 kb on + strand, within nadC at 118.355 kb on - strand, within nadC at 118.355 kb on - strand, within nadC at 118.364 kb on + strand, within nadC at 118.382 kb on - strand, within nadC at 118.538 kb on + strand, within nadC
Per-strain Table
Position Strand Gene LocusTag Fraction Vanillin 5 mM remove 116,149 - hofB b0107 0.31 +0.5 116,335 + hofB b0107 0.45 +0.3 116,399 + hofB b0107 0.49 -1.1 116,430 - hofB b0107 0.52 +0.4 116,430 - hofB b0107 0.52 -1.6 116,471 - hofB b0107 0.55 +0.7 116,760 - hofB b0107 0.75 +0.4 116,777 + hofB b0107 0.77 +0.6 116,777 + hofB b0107 0.77 -1.1 116,872 + hofB b0107 0.84 -0.1 116,882 + hofB b0107 0.84 +0.1 116,957 - hofB b0107 0.90 -0.1 116,964 - +0.1 117,046 + -0.3 117,056 + -0.1 117,069 + -0.4 117,075 + +0.0 117,075 + -1.8 117,075 + +0.6 117,081 - +0.4 117,081 - +0.8 117,084 + +0.5 117,087 + -0.0 117,227 - ppdD b0108 0.27 -0.4 117,281 + ppdD b0108 0.39 -1.6 117,281 + ppdD b0108 0.39 +0.6 117,293 - ppdD b0108 0.42 -0.4 117,302 + ppdD b0108 0.44 +0.3 117,302 + ppdD b0108 0.44 +0.0 117,385 + ppdD b0108 0.63 -0.1 117,387 + ppdD b0108 0.63 -1.8 117,508 - +1.6 117,508 - +0.0 117,511 - -0.4 117,511 - +0.4 117,525 - +1.0 117,525 - +0.0 117,603 + +0.3 117,603 + -1.4 117,839 + +0.3 117,856 - nadC b0109 0.12 +1.6 117,856 - nadC b0109 0.12 -1.0 117,879 - nadC b0109 0.14 -0.4 117,879 - nadC b0109 0.14 +0.4 117,881 - nadC b0109 0.14 +0.9 117,922 - nadC b0109 0.19 -0.9 117,922 - nadC b0109 0.19 +0.4 118,107 - nadC b0109 0.40 -0.0 118,201 + nadC b0109 0.50 +0.1 118,230 + nadC b0109 0.53 -0.6 118,230 + nadC b0109 0.53 +0.0 118,296 + nadC b0109 0.61 -1.9 118,355 - nadC b0109 0.67 +1.6 118,355 - nadC b0109 0.67 +0.3 118,364 + nadC b0109 0.68 -0.6 118,382 - nadC b0109 0.70 -1.7 118,538 + nadC b0109 0.88 +0.6
Or see this region's nucleotide sequence