Experiment: NAG (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0027 and Atu0028 overlap by 4 nucleotides Atu0028 and Atu0029 are separated by 44 nucleotides Atu0029 and Atu0030 are separated by 128 nucleotides Atu0030 and Atu0031 are separated by 11 nucleotides Atu0031 and Atu0032 are separated by 125 nucleotides
Atu0027: Atu0027 - two component sensor kinase, at 29,028 to 31,673
Atu0027
Atu0028: Atu0028 - hypothetical protein, at 31,670 to 31,837
Atu0028
Atu0029: Atu0029 - S-adenosylhomocysteine hydrolase, at 31,882 to 33,282
Atu0029
Atu0030: Atu0030 - phosphocarrier protein HPr, at 33,411 to 33,686
Atu0030
Atu0031: Atu0031 - PTS system, IIA component, at 33,698 to 34,099
Atu0031
Atu0032: Atu0032 - hypothetical protein, at 34,225 to 34,689
Atu0032
Position (kb) 31
32
33
34 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 32.177 kb on + strand, within Atu0029 at 32.234 kb on + strand, within Atu0029 at 32.236 kb on - strand, within Atu0029 at 32.419 kb on + strand, within Atu0029 at 32.534 kb on - strand, within Atu0029 at 32.770 kb on - strand, within Atu0029 at 32.770 kb on - strand, within Atu0029 at 32.802 kb on - strand, within Atu0029 at 32.862 kb on + strand, within Atu0029 at 32.971 kb on + strand, within Atu0029 at 33.222 kb on - strand at 33.251 kb on - strand at 33.349 kb on + strand at 33.357 kb on - strand at 33.409 kb on + strand at 33.416 kb on + strand at 33.416 kb on - strand at 33.416 kb on - strand at 33.523 kb on + strand, within Atu0030 at 33.665 kb on + strand at 33.773 kb on + strand, within Atu0031 at 34.084 kb on + strand at 34.092 kb on - strand at 34.108 kb on + strand at 34.202 kb on + strand at 34.210 kb on - strand at 34.228 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction NAG (C) remove 32,177 + Atu0029 0.21 +0.7 32,234 + Atu0029 0.25 -1.8 32,236 - Atu0029 0.25 -2.2 32,419 + Atu0029 0.38 -1.7 32,534 - Atu0029 0.47 -2.8 32,770 - Atu0029 0.63 -1.6 32,770 - Atu0029 0.63 -1.7 32,802 - Atu0029 0.66 -2.2 32,862 + Atu0029 0.70 -1.1 32,971 + Atu0029 0.78 -1.0 33,222 - -2.0 33,251 - -1.0 33,349 + +0.5 33,357 - -0.6 33,409 + +0.8 33,416 + -1.2 33,416 - -0.4 33,416 - +0.3 33,523 + Atu0030 0.41 -4.9 33,665 + -5.2 33,773 + Atu0031 0.19 -4.6 34,084 + -1.7 34,092 - -3.3 34,108 + -2.2 34,202 + +0.4 34,210 - +0.2 34,228 + +0.5
Or see this region's nucleotide sequence