Experiment: 5-Hydroxymethylfurfural 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phnP and phnO are separated by 1 nucleotides phnO and phnN overlap by 14 nucleotides phnN and phnM overlap by 1 nucleotides
b4092: phnP - carbon-phosphorus lyase complex accessory protein (NCBI), at 4,312,367 to 4,313,125
phnP
b4093: phnO - predicted acyltransferase with acyl-CoA N-acyltransferase domain (NCBI), at 4,313,127 to 4,313,561
phnO
b4094: phnN - ribose 1,5-bisphosphokinase (NCBI), at 4,313,548 to 4,314,105
phnN
b4095: phnM - carbon-phosphorus lyase complex subunit (NCBI), at 4,314,105 to 4,315,241
phnM
Position (kb)
4313
4314
4315 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4312.567 kb on + strand, within phnP at 4312.658 kb on - strand, within phnP at 4312.658 kb on - strand, within phnP at 4312.663 kb on - strand, within phnP at 4312.663 kb on - strand, within phnP at 4312.668 kb on - strand, within phnP at 4312.668 kb on - strand, within phnP at 4312.700 kb on + strand, within phnP at 4312.714 kb on - strand, within phnP at 4312.720 kb on + strand, within phnP at 4312.720 kb on + strand, within phnP at 4312.721 kb on - strand, within phnP at 4312.728 kb on - strand, within phnP at 4312.788 kb on - strand, within phnP at 4312.803 kb on + strand, within phnP at 4312.808 kb on + strand, within phnP at 4312.948 kb on - strand, within phnP at 4312.965 kb on - strand, within phnP at 4313.024 kb on - strand, within phnP at 4313.041 kb on + strand, within phnP at 4313.060 kb on + strand at 4313.094 kb on - strand at 4313.115 kb on - strand at 4313.115 kb on - strand at 4313.127 kb on + strand at 4313.185 kb on - strand, within phnO at 4313.195 kb on - strand, within phnO at 4313.274 kb on + strand, within phnO at 4313.433 kb on + strand, within phnO at 4313.502 kb on - strand, within phnO at 4313.502 kb on - strand, within phnO at 4313.529 kb on - strand at 4313.529 kb on - strand at 4313.565 kb on - strand at 4313.634 kb on + strand, within phnN at 4313.662 kb on - strand, within phnN at 4313.821 kb on + strand, within phnN at 4313.821 kb on + strand, within phnN at 4313.881 kb on - strand, within phnN at 4313.881 kb on - strand, within phnN at 4313.956 kb on - strand, within phnN at 4314.001 kb on - strand, within phnN at 4314.043 kb on + strand, within phnN at 4314.122 kb on - strand at 4314.213 kb on + strand at 4314.213 kb on + strand at 4314.357 kb on - strand, within phnM at 4314.363 kb on + strand, within phnM at 4314.396 kb on + strand, within phnM at 4314.445 kb on + strand, within phnM at 4314.445 kb on + strand, within phnM at 4314.742 kb on + strand, within phnM at 4314.819 kb on + strand, within phnM at 4314.819 kb on + strand, within phnM at 4314.822 kb on - strand, within phnM at 4314.822 kb on - strand, within phnM at 4314.857 kb on - strand, within phnM at 4314.857 kb on - strand, within phnM at 4315.006 kb on - strand, within phnM
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 20 mM remove 4,312,567 + phnP b4092 0.26 +1.5 4,312,658 - phnP b4092 0.38 +0.7 4,312,658 - phnP b4092 0.38 +1.3 4,312,663 - phnP b4092 0.39 +1.2 4,312,663 - phnP b4092 0.39 -0.7 4,312,668 - phnP b4092 0.40 -0.1 4,312,668 - phnP b4092 0.40 +0.0 4,312,700 + phnP b4092 0.44 +0.3 4,312,714 - phnP b4092 0.46 +0.6 4,312,720 + phnP b4092 0.47 -0.8 4,312,720 + phnP b4092 0.47 +0.5 4,312,721 - phnP b4092 0.47 +1.9 4,312,728 - phnP b4092 0.48 -0.2 4,312,788 - phnP b4092 0.55 +0.2 4,312,803 + phnP b4092 0.57 +0.5 4,312,808 + phnP b4092 0.58 -0.6 4,312,948 - phnP b4092 0.77 +0.5 4,312,965 - phnP b4092 0.79 +0.1 4,313,024 - phnP b4092 0.87 +0.8 4,313,041 + phnP b4092 0.89 -1.0 4,313,060 + +0.7 4,313,094 - +1.7 4,313,115 - -1.5 4,313,115 - +0.2 4,313,127 + +0.2 4,313,185 - phnO b4093 0.13 +1.5 4,313,195 - phnO b4093 0.16 -0.3 4,313,274 + phnO b4093 0.34 -1.0 4,313,433 + phnO b4093 0.70 -0.3 4,313,502 - phnO b4093 0.86 +1.5 4,313,502 - phnO b4093 0.86 -0.1 4,313,529 - -0.1 4,313,529 - -0.1 4,313,565 - +1.5 4,313,634 + phnN b4094 0.15 -1.0 4,313,662 - phnN b4094 0.20 -0.7 4,313,821 + phnN b4094 0.49 -1.2 4,313,821 + phnN b4094 0.49 -1.3 4,313,881 - phnN b4094 0.60 +0.5 4,313,881 - phnN b4094 0.60 -0.5 4,313,956 - phnN b4094 0.73 -0.5 4,314,001 - phnN b4094 0.81 -0.4 4,314,043 + phnN b4094 0.89 +0.5 4,314,122 - +0.4 4,314,213 + +0.5 4,314,213 + +0.8 4,314,357 - phnM b4095 0.22 -0.0 4,314,363 + phnM b4095 0.23 -0.1 4,314,396 + phnM b4095 0.26 -0.1 4,314,445 + phnM b4095 0.30 -0.6 4,314,445 + phnM b4095 0.30 +0.9 4,314,742 + phnM b4095 0.56 -2.1 4,314,819 + phnM b4095 0.63 -0.5 4,314,819 + phnM b4095 0.63 +0.5 4,314,822 - phnM b4095 0.63 +0.5 4,314,822 - phnM b4095 0.63 -1.2 4,314,857 - phnM b4095 0.66 -0.9 4,314,857 - phnM b4095 0.66 -0.2 4,315,006 - phnM b4095 0.79 +0.5
Or see this region's nucleotide sequence