Experiment: 5-Hydroxymethylfurfural 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt recC and ppdC are separated by 12 nucleotides ppdC and ygdB overlap by 16 nucleotides ygdB and ppdB overlap by 4 nucleotides ppdB and ppdA overlap by 10 nucleotides
b2822: recC - exonuclease V (RecBCD complex), gamma chain (NCBI), at 2,957,082 to 2,960,450
recC
b2823: ppdC - hypothetical protein (NCBI), at 2,960,463 to 2,960,786
ppdC
b2824: ygdB - orf, hypothetical protein (VIMSS), at 2,960,771 to 2,961,178
ygdB
b2825: ppdB - hypothetical protein (NCBI), at 2,961,175 to 2,961,738
ppdB
b2826: ppdA - hypothetical protein (NCBI), at 2,961,729 to 2,962,199
ppdA
Position (kb)
2960
2961
2962 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2960.337 kb on - strand at 2960.370 kb on + strand at 2960.441 kb on + strand at 2960.489 kb on + strand at 2960.523 kb on - strand, within ppdC at 2960.523 kb on - strand, within ppdC at 2960.535 kb on + strand, within ppdC at 2960.661 kb on + strand, within ppdC at 2960.726 kb on - strand, within ppdC at 2960.726 kb on - strand, within ppdC at 2960.736 kb on + strand, within ppdC at 2960.759 kb on - strand at 2960.759 kb on - strand at 2960.974 kb on - strand, within ygdB at 2961.100 kb on + strand, within ygdB at 2961.100 kb on + strand, within ygdB at 2961.102 kb on - strand, within ygdB at 2961.141 kb on + strand at 2961.173 kb on + strand at 2961.343 kb on - strand, within ppdB at 2961.469 kb on + strand, within ppdB at 2961.469 kb on + strand, within ppdB at 2961.483 kb on - strand, within ppdB at 2961.483 kb on - strand, within ppdB at 2961.493 kb on - strand, within ppdB at 2961.493 kb on - strand, within ppdB at 2961.518 kb on + strand, within ppdB at 2961.529 kb on - strand, within ppdB at 2961.529 kb on - strand, within ppdB at 2961.543 kb on - strand, within ppdB at 2961.543 kb on - strand, within ppdB at 2961.543 kb on - strand, within ppdB at 2961.664 kb on + strand, within ppdB at 2961.664 kb on + strand, within ppdB at 2961.794 kb on + strand, within ppdA at 2961.794 kb on + strand, within ppdA at 2961.832 kb on - strand, within ppdA at 2961.832 kb on - strand, within ppdA at 2961.852 kb on - strand, within ppdA at 2961.875 kb on + strand, within ppdA at 2961.875 kb on + strand, within ppdA at 2961.906 kb on + strand, within ppdA at 2961.918 kb on + strand, within ppdA at 2961.943 kb on + strand, within ppdA at 2961.943 kb on + strand, within ppdA at 2961.958 kb on + strand, within ppdA at 2961.958 kb on + strand, within ppdA at 2961.963 kb on + strand, within ppdA at 2962.113 kb on + strand, within ppdA at 2962.122 kb on - strand, within ppdA at 2962.130 kb on + strand, within ppdA at 2962.130 kb on + strand, within ppdA at 2962.149 kb on - strand, within ppdA at 2962.149 kb on - strand, within ppdA at 2962.151 kb on + strand, within ppdA at 2962.173 kb on - strand at 2962.175 kb on + strand at 2962.175 kb on + strand at 2962.175 kb on + strand at 2962.175 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 20 mM remove 2,960,337 - -1.4 2,960,370 + -0.4 2,960,441 + -0.4 2,960,489 + +0.3 2,960,523 - ppdC b2823 0.19 +2.1 2,960,523 - ppdC b2823 0.19 -0.9 2,960,535 + ppdC b2823 0.22 +0.2 2,960,661 + ppdC b2823 0.61 +0.0 2,960,726 - ppdC b2823 0.81 -0.4 2,960,726 - ppdC b2823 0.81 +0.1 2,960,736 + ppdC b2823 0.84 -0.3 2,960,759 - +0.1 2,960,759 - +0.1 2,960,974 - ygdB b2824 0.50 -0.2 2,961,100 + ygdB b2824 0.81 -1.1 2,961,100 + ygdB b2824 0.81 -1.9 2,961,102 - ygdB b2824 0.81 +1.1 2,961,141 + +0.4 2,961,173 + +1.1 2,961,343 - ppdB b2825 0.30 +0.3 2,961,469 + ppdB b2825 0.52 +0.1 2,961,469 + ppdB b2825 0.52 +1.9 2,961,483 - ppdB b2825 0.55 +0.1 2,961,483 - ppdB b2825 0.55 +0.0 2,961,493 - ppdB b2825 0.56 +0.7 2,961,493 - ppdB b2825 0.56 -0.1 2,961,518 + ppdB b2825 0.61 -1.0 2,961,529 - ppdB b2825 0.63 +1.1 2,961,529 - ppdB b2825 0.63 -0.4 2,961,543 - ppdB b2825 0.65 -0.4 2,961,543 - ppdB b2825 0.65 +0.1 2,961,543 - ppdB b2825 0.65 -0.1 2,961,664 + ppdB b2825 0.87 +0.1 2,961,664 + ppdB b2825 0.87 -0.0 2,961,794 + ppdA b2826 0.14 +0.3 2,961,794 + ppdA b2826 0.14 -0.4 2,961,832 - ppdA b2826 0.22 -1.2 2,961,832 - ppdA b2826 0.22 +0.1 2,961,852 - ppdA b2826 0.26 -0.2 2,961,875 + ppdA b2826 0.31 -0.3 2,961,875 + ppdA b2826 0.31 +0.9 2,961,906 + ppdA b2826 0.38 +0.5 2,961,918 + ppdA b2826 0.40 +0.4 2,961,943 + ppdA b2826 0.45 +1.3 2,961,943 + ppdA b2826 0.45 +0.7 2,961,958 + ppdA b2826 0.49 +0.7 2,961,958 + ppdA b2826 0.49 +1.1 2,961,963 + ppdA b2826 0.50 -0.7 2,962,113 + ppdA b2826 0.82 -0.0 2,962,122 - ppdA b2826 0.83 -1.2 2,962,130 + ppdA b2826 0.85 +0.7 2,962,130 + ppdA b2826 0.85 -0.3 2,962,149 - ppdA b2826 0.89 -1.2 2,962,149 - ppdA b2826 0.89 +2.7 2,962,151 + ppdA b2826 0.90 +0.7 2,962,173 - -0.1 2,962,175 + +1.1 2,962,175 + -0.7 2,962,175 + +0.1 2,962,175 + +0.8
Or see this region's nucleotide sequence