Experiment: 5-Hydroxymethylfurfural 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt astE and astB overlap by 8 nucleotides astB and astD overlap by 4 nucleotides
b1744: astE - succinylglutamate desuccinylase (NCBI), at 1,823,979 to 1,824,947
astE
b1745: astB - succinylarginine dihydrolase (NCBI), at 1,824,940 to 1,826,283
astB
b1746: astD - succinylglutamic semialdehyde dehydrogenase (NCBI), at 1,826,280 to 1,827,758
astD
Position (kb)
1824
1825
1826
1827 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1824.017 kb on - strand at 1824.017 kb on - strand at 1824.032 kb on + strand at 1824.047 kb on - strand at 1824.047 kb on - strand at 1824.052 kb on - strand at 1824.053 kb on - strand at 1824.059 kb on - strand at 1824.059 kb on - strand at 1824.087 kb on + strand, within astE at 1824.090 kb on - strand, within astE at 1824.090 kb on - strand, within astE at 1824.095 kb on - strand, within astE at 1824.095 kb on - strand, within astE at 1824.095 kb on - strand, within astE at 1824.095 kb on - strand, within astE at 1824.330 kb on + strand, within astE at 1824.333 kb on - strand, within astE at 1824.380 kb on + strand, within astE at 1824.397 kb on + strand, within astE at 1824.512 kb on + strand, within astE at 1824.650 kb on - strand, within astE at 1824.857 kb on + strand at 1824.871 kb on - strand at 1825.089 kb on - strand, within astB at 1825.285 kb on + strand, within astB at 1825.285 kb on + strand, within astB at 1825.293 kb on - strand, within astB at 1825.321 kb on - strand, within astB at 1825.321 kb on - strand, within astB at 1825.378 kb on + strand, within astB at 1825.727 kb on - strand, within astB at 1825.767 kb on - strand, within astB at 1826.034 kb on + strand, within astB at 1826.113 kb on - strand, within astB at 1826.358 kb on + strand at 1826.451 kb on - strand, within astD at 1826.451 kb on - strand, within astD at 1826.451 kb on - strand, within astD at 1826.614 kb on + strand, within astD at 1826.707 kb on + strand, within astD at 1826.730 kb on - strand, within astD at 1826.730 kb on - strand, within astD at 1826.786 kb on + strand, within astD at 1826.848 kb on - strand, within astD at 1826.848 kb on - strand, within astD at 1826.870 kb on - strand, within astD at 1826.949 kb on - strand, within astD at 1826.949 kb on - strand, within astD at 1827.115 kb on + strand, within astD at 1827.262 kb on - strand, within astD at 1827.262 kb on - strand, within astD
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 20 mM remove 1,824,017 - +0.4 1,824,017 - -1.6 1,824,032 + +0.5 1,824,047 - -0.5 1,824,047 - -0.8 1,824,052 - -0.2 1,824,053 - +1.0 1,824,059 - -0.3 1,824,059 - -0.5 1,824,087 + astE b1744 0.11 -0.8 1,824,090 - astE b1744 0.11 +0.1 1,824,090 - astE b1744 0.11 +0.2 1,824,095 - astE b1744 0.12 +0.7 1,824,095 - astE b1744 0.12 -0.5 1,824,095 - astE b1744 0.12 +1.0 1,824,095 - astE b1744 0.12 -0.8 1,824,330 + astE b1744 0.36 +1.1 1,824,333 - astE b1744 0.37 +0.0 1,824,380 + astE b1744 0.41 +0.7 1,824,397 + astE b1744 0.43 -0.5 1,824,512 + astE b1744 0.55 -0.2 1,824,650 - astE b1744 0.69 -1.1 1,824,857 + +0.6 1,824,871 - +1.7 1,825,089 - astB b1745 0.11 +0.7 1,825,285 + astB b1745 0.26 +1.1 1,825,285 + astB b1745 0.26 -0.7 1,825,293 - astB b1745 0.26 -1.3 1,825,321 - astB b1745 0.28 -0.3 1,825,321 - astB b1745 0.28 -0.8 1,825,378 + astB b1745 0.33 +0.7 1,825,727 - astB b1745 0.59 -0.9 1,825,767 - astB b1745 0.62 +0.4 1,826,034 + astB b1745 0.81 +0.5 1,826,113 - astB b1745 0.87 +1.1 1,826,358 + +0.1 1,826,451 - astD b1746 0.12 +0.5 1,826,451 - astD b1746 0.12 +0.9 1,826,451 - astD b1746 0.12 -0.9 1,826,614 + astD b1746 0.23 +0.1 1,826,707 + astD b1746 0.29 +1.1 1,826,730 - astD b1746 0.30 +1.7 1,826,730 - astD b1746 0.30 +0.6 1,826,786 + astD b1746 0.34 -0.9 1,826,848 - astD b1746 0.38 -0.3 1,826,848 - astD b1746 0.38 +2.7 1,826,870 - astD b1746 0.40 +0.1 1,826,949 - astD b1746 0.45 -0.9 1,826,949 - astD b1746 0.45 +0.7 1,827,115 + astD b1746 0.56 -2.1 1,827,262 - astD b1746 0.66 -0.0 1,827,262 - astD b1746 0.66 +0.1
Or see this region's nucleotide sequence