Experiment: 5-Hydroxymethylfurfural 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mdoD and ydcH are separated by 139 nucleotides ydcH and rimL are separated by 62 nucleotides rimL and ydcK overlap by 9 nucleotides
b1424: mdoD - glucan biosynthesis protein, periplasmic (RefSeq), at 1,494,880 to 1,496,535
mdoD
b1426: ydcH - orf, hypothetical protein (VIMSS), at 1,496,675 to 1,496,899
ydcH
b1427: rimL - ribosomal-protein-L7/L12-serine acetyltransferase (NCBI), at 1,496,962 to 1,497,501
rimL
b1428: ydcK - predicted enzyme (NCBI), at 1,497,493 to 1,498,473
ydcK
Position (kb)
1496
1497 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1495.719 kb on + strand, within mdoD at 1495.895 kb on - strand, within mdoD at 1496.356 kb on - strand, within mdoD at 1496.356 kb on - strand, within mdoD at 1496.405 kb on + strand at 1496.405 kb on + strand at 1496.405 kb on + strand at 1496.413 kb on - strand at 1496.413 kb on - strand at 1496.413 kb on - strand at 1496.669 kb on - strand at 1496.669 kb on - strand at 1496.673 kb on - strand at 1496.808 kb on - strand, within ydcH at 1496.896 kb on - strand at 1497.142 kb on - strand, within rimL at 1497.231 kb on + strand, within rimL at 1497.266 kb on + strand, within rimL at 1497.328 kb on - strand, within rimL at 1497.407 kb on + strand, within rimL at 1497.636 kb on + strand, within ydcK at 1497.662 kb on + strand, within ydcK at 1497.751 kb on - strand, within ydcK at 1497.751 kb on - strand, within ydcK at 1497.751 kb on - strand, within ydcK at 1497.765 kb on + strand, within ydcK at 1497.766 kb on + strand, within ydcK at 1497.792 kb on - strand, within ydcK at 1497.805 kb on + strand, within ydcK at 1497.829 kb on - strand, within ydcK
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 20 mM remove 1,495,719 + mdoD b1424 0.51 -0.4 1,495,895 - mdoD b1424 0.61 -0.4 1,496,356 - mdoD b1424 0.89 -0.4 1,496,356 - mdoD b1424 0.89 -2.0 1,496,405 + +0.6 1,496,405 + -1.0 1,496,405 + -1.0 1,496,413 - +0.9 1,496,413 - +0.4 1,496,413 - -1.0 1,496,669 - -0.4 1,496,669 - -0.7 1,496,673 - -0.5 1,496,808 - ydcH b1426 0.59 -0.7 1,496,896 - -1.2 1,497,142 - rimL b1427 0.33 +1.0 1,497,231 + rimL b1427 0.50 +1.9 1,497,266 + rimL b1427 0.56 +0.2 1,497,328 - rimL b1427 0.68 +2.2 1,497,407 + rimL b1427 0.82 -2.0 1,497,636 + ydcK b1428 0.15 +1.9 1,497,662 + ydcK b1428 0.17 +0.4 1,497,751 - ydcK b1428 0.26 -0.9 1,497,751 - ydcK b1428 0.26 +1.3 1,497,751 - ydcK b1428 0.26 +1.8 1,497,765 + ydcK b1428 0.28 +0.4 1,497,766 + ydcK b1428 0.28 +0.4 1,497,792 - ydcK b1428 0.30 -1.4 1,497,805 + ydcK b1428 0.32 +0.3 1,497,829 - ydcK b1428 0.34 +0.6
Or see this region's nucleotide sequence