Strain Fitness in Escherichia coli BW25113 around b2935

Experiment: 2-Furfuraldehyde 0.25 vol%

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntcmtA and cmtB are separated by 27 nucleotidescmtB and tktA are separated by 313 nucleotidestktA and yggG are separated by 277 nucleotides b2933: cmtA - predicted fused mannitol-specific PTS enzymes: IIB component/IIC component (NCBI), at 3,075,493 to 3,076,881 cmtA b2934: cmtB - predicted mannitol-specific enzyme IIA component of PTS (NCBI), at 3,076,909 to 3,077,352 cmtB b2935: tktA - transketolase 1, thiamin-binding (NCBI), at 3,077,666 to 3,079,657 tktA b2936: yggG - orf, hypothetical protein (VIMSS), at 3,079,935 to 3,080,693 yggG Position (kb) 3077 3078 3079 3080Strain fitness (log2 ratio) -1 0 1 2 3at 3076.668 kb on + strand, within cmtAat 3076.668 kb on + strand, within cmtAat 3076.676 kb on + strand, within cmtAat 3076.676 kb on + strand, within cmtAat 3076.676 kb on + strand, within cmtAat 3076.677 kb on + strand, within cmtAat 3076.677 kb on + strand, within cmtAat 3076.701 kb on + strand, within cmtAat 3076.750 kb on + strandat 3076.750 kb on + strandat 3076.754 kb on + strandat 3076.754 kb on + strandat 3076.813 kb on - strandat 3076.837 kb on + strandat 3076.837 kb on - strandat 3076.841 kb on + strandat 3076.853 kb on + strandat 3076.854 kb on + strandat 3076.854 kb on + strandat 3077.141 kb on - strand, within cmtBat 3077.180 kb on + strand, within cmtBat 3077.305 kb on + strand, within cmtBat 3077.305 kb on + strand, within cmtBat 3077.438 kb on - strandat 3077.496 kb on - strandat 3077.496 kb on - strandat 3077.516 kb on + strandat 3077.531 kb on + strandat 3077.531 kb on + strandat 3077.576 kb on + strandat 3077.596 kb on - strandat 3077.596 kb on - strandat 3077.617 kb on - strandat 3077.695 kb on - strandat 3077.707 kb on - strandat 3077.717 kb on - strandat 3077.778 kb on - strandat 3077.778 kb on - strandat 3078.018 kb on + strand, within tktAat 3078.067 kb on + strand, within tktAat 3078.104 kb on + strand, within tktAat 3078.233 kb on + strand, within tktAat 3078.406 kb on - strand, within tktAat 3078.580 kb on + strand, within tktAat 3078.776 kb on + strand, within tktAat 3079.066 kb on - strand, within tktAat 3079.341 kb on - strand, within tktAat 3079.494 kb on + strandat 3079.549 kb on - strandat 3079.762 kb on + strandat 3079.812 kb on - strandat 3079.870 kb on + strandat 3079.977 kb on + strandat 3079.985 kb on - strandat 3080.005 kb on + strandat 3080.126 kb on + strand, within yggGat 3080.177 kb on + strand, within yggGat 3080.197 kb on - strand, within yggGat 3080.220 kb on - strand, within yggGat 3080.220 kb on - strand, within yggGat 3080.246 kb on + strand, within yggGat 3080.275 kb on + strand, within yggGat 3080.302 kb on - strand, within yggGat 3080.340 kb on + strand, within yggGat 3080.348 kb on - strand, within yggGat 3080.540 kb on + strand, within yggGat 3080.553 kb on - strand, within yggGat 3080.626 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 2-Furfuraldehyde 0.25 vol%
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3,076,668 + cmtA b2933 0.85 -0.3
3,076,668 + cmtA b2933 0.85 +0.9
3,076,676 + cmtA b2933 0.85 +0.3
3,076,676 + cmtA b2933 0.85 -0.5
3,076,676 + cmtA b2933 0.85 -0.2
3,076,677 + cmtA b2933 0.85 -0.9
3,076,677 + cmtA b2933 0.85 -1.3
3,076,701 + cmtA b2933 0.87 -0.4
3,076,750 + +0.7
3,076,750 + -0.3
3,076,754 + +1.3
3,076,754 + -1.5
3,076,813 - +0.6
3,076,837 + +1.3
3,076,837 - -0.8
3,076,841 + +0.7
3,076,853 + +0.3
3,076,854 + +0.3
3,076,854 + +0.4
3,077,141 - cmtB b2934 0.52 +2.8
3,077,180 + cmtB b2934 0.61 -0.4
3,077,305 + cmtB b2934 0.89 -0.0
3,077,305 + cmtB b2934 0.89 -0.7
3,077,438 - -1.2
3,077,496 - +0.6
3,077,496 - -0.1
3,077,516 + +1.1
3,077,531 + -0.5
3,077,531 + -0.5
3,077,576 + +0.1
3,077,596 - +0.1
3,077,596 - +1.3
3,077,617 - -0.3
3,077,695 - +2.2
3,077,707 - +1.1
3,077,717 - +1.4
3,077,778 - -0.5
3,077,778 - -1.3
3,078,018 + tktA b2935 0.18 +1.1
3,078,067 + tktA b2935 0.20 -1.5
3,078,104 + tktA b2935 0.22 -1.7
3,078,233 + tktA b2935 0.28 +1.6
3,078,406 - tktA b2935 0.37 -1.3
3,078,580 + tktA b2935 0.46 -1.3
3,078,776 + tktA b2935 0.56 -0.5
3,079,066 - tktA b2935 0.70 -1.3
3,079,341 - tktA b2935 0.84 -1.3
3,079,494 + -1.5
3,079,549 - -0.5
3,079,762 + +1.0
3,079,812 - +1.1
3,079,870 + +0.5
3,079,977 + +1.1
3,079,985 - -0.3
3,080,005 + +1.1
3,080,126 + yggG b2936 0.25 +0.2
3,080,177 + yggG b2936 0.32 +0.1
3,080,197 - yggG b2936 0.35 -0.3
3,080,220 - yggG b2936 0.38 +0.1
3,080,220 - yggG b2936 0.38 +1.1
3,080,246 + yggG b2936 0.41 -1.3
3,080,275 + yggG b2936 0.45 -1.7
3,080,302 - yggG b2936 0.48 +0.5
3,080,340 + yggG b2936 0.53 -0.8
3,080,348 - yggG b2936 0.54 -0.1
3,080,540 + yggG b2936 0.80 -0.1
3,080,553 - yggG b2936 0.81 -0.6
3,080,626 - +0.7

Or see this region's nucleotide sequence