Experiment: 2-Furfuraldehyde 0.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt wcaH and fcl are separated by 2 nucleotides fcl and gmd are separated by 2 nucleotides gmd and wcaF are separated by 25 nucleotides wcaF and wcaE are separated by 15 nucleotides
b2051: wcaH - GDP-mannose mannosyl hydrolase (VIMSS), at 2,123,767 to 2,124,246
wcaH
b2052: fcl - bifunctional GDP-fucose synthetase: GDP-4-dehydro-6-deoxy-D-mannose epimerase/ GDP-4-dehydro-6-L-deoxygalactose reductase (NCBI), at 2,124,249 to 2,125,214
fcl
b2053: gmd - GDP-D-mannose dehydratase, NAD(P)-binding (NCBI), at 2,125,217 to 2,126,338
gmd
b2054: wcaF - predicted acyl transferase (NCBI), at 2,126,364 to 2,126,912
wcaF
b2055: wcaE - predicted glycosyl transferase (NCBI), at 2,126,928 to 2,127,674
wcaE
Position (kb)
2125
2126
2127 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2124.282 kb on + strand at 2124.282 kb on + strand at 2124.465 kb on + strand, within fcl at 2124.601 kb on + strand, within fcl at 2124.630 kb on + strand, within fcl at 2124.630 kb on + strand, within fcl at 2124.722 kb on - strand, within fcl at 2124.813 kb on - strand, within fcl at 2124.848 kb on - strand, within fcl at 2124.896 kb on + strand, within fcl at 2124.951 kb on + strand, within fcl at 2124.951 kb on + strand, within fcl at 2125.046 kb on - strand, within fcl at 2125.113 kb on + strand, within fcl at 2125.189 kb on + strand at 2125.221 kb on - strand at 2125.556 kb on + strand, within gmd at 2125.566 kb on + strand, within gmd at 2125.570 kb on + strand, within gmd at 2125.570 kb on + strand, within gmd at 2125.643 kb on + strand, within gmd at 2125.706 kb on + strand, within gmd at 2125.707 kb on - strand, within gmd at 2125.797 kb on + strand, within gmd at 2125.797 kb on + strand, within gmd at 2125.828 kb on + strand, within gmd at 2125.887 kb on + strand, within gmd at 2125.941 kb on - strand, within gmd at 2126.048 kb on + strand, within gmd at 2126.090 kb on - strand, within gmd at 2126.093 kb on - strand, within gmd at 2126.151 kb on + strand, within gmd at 2126.181 kb on - strand, within gmd at 2126.225 kb on - strand, within gmd at 2126.225 kb on - strand, within gmd at 2126.247 kb on - strand at 2126.247 kb on - strand at 2126.247 kb on - strand at 2126.267 kb on + strand at 2126.388 kb on + strand at 2126.398 kb on - strand at 2126.451 kb on - strand at 2126.451 kb on - strand, within wcaF at 2126.456 kb on - strand, within wcaF at 2126.456 kb on - strand, within wcaF at 2126.498 kb on + strand, within wcaF at 2126.515 kb on + strand, within wcaF at 2126.550 kb on - strand, within wcaF at 2126.550 kb on - strand, within wcaF at 2126.562 kb on + strand, within wcaF at 2126.562 kb on - strand, within wcaF at 2126.562 kb on - strand, within wcaF at 2126.577 kb on + strand, within wcaF at 2126.577 kb on + strand, within wcaF at 2126.584 kb on - strand, within wcaF at 2126.587 kb on + strand, within wcaF at 2126.614 kb on + strand, within wcaF at 2126.616 kb on + strand, within wcaF at 2126.621 kb on + strand, within wcaF at 2126.629 kb on + strand, within wcaF at 2126.660 kb on - strand, within wcaF at 2126.681 kb on + strand, within wcaF at 2126.681 kb on + strand, within wcaF at 2126.914 kb on + strand at 2127.243 kb on - strand, within wcaE
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Furfuraldehyde 0.25 vol% remove 2,124,282 + +0.5 2,124,282 + -0.1 2,124,465 + fcl b2052 0.22 +1.7 2,124,601 + fcl b2052 0.36 -0.9 2,124,630 + fcl b2052 0.39 -0.1 2,124,630 + fcl b2052 0.39 +0.9 2,124,722 - fcl b2052 0.49 +0.1 2,124,813 - fcl b2052 0.58 -0.1 2,124,848 - fcl b2052 0.62 +1.6 2,124,896 + fcl b2052 0.67 +1.0 2,124,951 + fcl b2052 0.73 +0.4 2,124,951 + fcl b2052 0.73 +0.4 2,125,046 - fcl b2052 0.83 -0.4 2,125,113 + fcl b2052 0.89 -0.6 2,125,189 + +0.2 2,125,221 - +0.6 2,125,556 + gmd b2053 0.30 +0.5 2,125,566 + gmd b2053 0.31 +0.6 2,125,570 + gmd b2053 0.31 +0.3 2,125,570 + gmd b2053 0.31 +0.4 2,125,643 + gmd b2053 0.38 +0.4 2,125,706 + gmd b2053 0.44 +0.1 2,125,707 - gmd b2053 0.44 +0.1 2,125,797 + gmd b2053 0.52 +0.9 2,125,797 + gmd b2053 0.52 -1.1 2,125,828 + gmd b2053 0.54 -0.7 2,125,887 + gmd b2053 0.60 -0.4 2,125,941 - gmd b2053 0.65 +0.6 2,126,048 + gmd b2053 0.74 +0.3 2,126,090 - gmd b2053 0.78 +0.9 2,126,093 - gmd b2053 0.78 +1.4 2,126,151 + gmd b2053 0.83 +1.6 2,126,181 - gmd b2053 0.86 -0.1 2,126,225 - gmd b2053 0.90 -0.3 2,126,225 - gmd b2053 0.90 +0.2 2,126,247 - +0.5 2,126,247 - -0.1 2,126,247 - +0.3 2,126,267 + -1.3 2,126,388 + -0.4 2,126,398 - -0.7 2,126,451 - +1.5 2,126,451 - wcaF b2054 0.16 -0.7 2,126,456 - wcaF b2054 0.17 -0.1 2,126,456 - wcaF b2054 0.17 -0.1 2,126,498 + wcaF b2054 0.24 +0.6 2,126,515 + wcaF b2054 0.28 -0.4 2,126,550 - wcaF b2054 0.34 +0.7 2,126,550 - wcaF b2054 0.34 +0.0 2,126,562 + wcaF b2054 0.36 +0.9 2,126,562 - wcaF b2054 0.36 -3.3 2,126,562 - wcaF b2054 0.36 +0.9 2,126,577 + wcaF b2054 0.39 -0.9 2,126,577 + wcaF b2054 0.39 -1.1 2,126,584 - wcaF b2054 0.40 +0.9 2,126,587 + wcaF b2054 0.41 -0.9 2,126,614 + wcaF b2054 0.46 +0.5 2,126,616 + wcaF b2054 0.46 +0.1 2,126,621 + wcaF b2054 0.47 -0.8 2,126,629 + wcaF b2054 0.48 +1.2 2,126,660 - wcaF b2054 0.54 -0.1 2,126,681 + wcaF b2054 0.58 -0.1 2,126,681 + wcaF b2054 0.58 +1.4 2,126,914 + +0.2 2,127,243 - wcaE b2055 0.42 +0.2
Or see this region's nucleotide sequence