Experiment: 2-Furfuraldehyde 0.25 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybdH and ybdL are separated by 108 nucleotides ybdL and ybdM are separated by 0 nucleotides ybdM and ybdN overlap by 28 nucleotides
b0599: ybdH - predicted oxidoreductase (NCBI), at 631,612 to 632,700
ybdH
b0600: ybdL - putative aminotransferase (NCBI), at 632,809 to 633,969
ybdL
b0601: ybdM - hypothetical protein (NCBI), at 633,970 to 634,599
ybdM
b0602: ybdN - hypothetical protein (NCBI), at 634,572 to 635,792
ybdN
Position (kb) 632
633
634 Strain fitness (log2 ratio) -2
-1
0
1
2 at 631.825 kb on + strand, within ybdH at 631.828 kb on - strand, within ybdH at 631.828 kb on - strand, within ybdH at 631.833 kb on - strand, within ybdH at 631.833 kb on - strand, within ybdH at 631.926 kb on + strand, within ybdH at 631.926 kb on - strand, within ybdH at 632.035 kb on + strand, within ybdH at 632.048 kb on + strand, within ybdH at 632.147 kb on + strand, within ybdH at 632.160 kb on + strand, within ybdH at 632.399 kb on + strand, within ybdH at 632.466 kb on - strand, within ybdH at 632.581 kb on + strand, within ybdH at 632.877 kb on - strand at 632.877 kb on - strand at 632.948 kb on + strand, within ybdL at 633.223 kb on + strand, within ybdL at 633.228 kb on + strand, within ybdL at 633.228 kb on + strand, within ybdL at 633.228 kb on - strand, within ybdL at 633.320 kb on - strand, within ybdL at 633.322 kb on - strand, within ybdL at 633.327 kb on - strand, within ybdL at 633.351 kb on - strand, within ybdL at 633.418 kb on + strand, within ybdL at 633.418 kb on - strand, within ybdL at 633.418 kb on - strand, within ybdL at 633.442 kb on - strand, within ybdL at 633.442 kb on - strand, within ybdL at 633.541 kb on - strand, within ybdL at 633.551 kb on - strand, within ybdL at 633.570 kb on - strand, within ybdL at 633.572 kb on + strand, within ybdL at 633.587 kb on + strand, within ybdL at 633.597 kb on - strand, within ybdL at 633.717 kb on - strand, within ybdL at 633.825 kb on + strand, within ybdL at 633.853 kb on - strand, within ybdL at 633.970 kb on + strand at 634.194 kb on + strand, within ybdM at 634.206 kb on + strand, within ybdM at 634.214 kb on - strand, within ybdM at 634.214 kb on - strand, within ybdM at 634.214 kb on - strand, within ybdM at 634.214 kb on - strand, within ybdM at 634.214 kb on - strand, within ybdM at 634.309 kb on + strand, within ybdM at 634.335 kb on - strand, within ybdM at 634.335 kb on - strand, within ybdM at 634.337 kb on - strand, within ybdM at 634.464 kb on - strand, within ybdM at 634.477 kb on - strand, within ybdM at 634.486 kb on - strand, within ybdM at 634.486 kb on - strand, within ybdM at 634.593 kb on + strand at 634.672 kb on + strand at 634.687 kb on + strand at 634.720 kb on - strand, within ybdN at 634.963 kb on + strand, within ybdN
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Furfuraldehyde 0.25 vol% remove 631,825 + ybdH b0599 0.20 -0.7 631,828 - ybdH b0599 0.20 +0.3 631,828 - ybdH b0599 0.20 -0.8 631,833 - ybdH b0599 0.20 -0.9 631,833 - ybdH b0599 0.20 +0.4 631,926 + ybdH b0599 0.29 -0.3 631,926 - ybdH b0599 0.29 +0.7 632,035 + ybdH b0599 0.39 -0.6 632,048 + ybdH b0599 0.40 +0.5 632,147 + ybdH b0599 0.49 +0.2 632,160 + ybdH b0599 0.50 +1.0 632,399 + ybdH b0599 0.72 +0.2 632,466 - ybdH b0599 0.78 +1.3 632,581 + ybdH b0599 0.89 +0.4 632,877 - +0.0 632,877 - +0.6 632,948 + ybdL b0600 0.12 +0.4 633,223 + ybdL b0600 0.36 +0.1 633,228 + ybdL b0600 0.36 -0.6 633,228 + ybdL b0600 0.36 +2.6 633,228 - ybdL b0600 0.36 -0.3 633,320 - ybdL b0600 0.44 +0.7 633,322 - ybdL b0600 0.44 +0.4 633,327 - ybdL b0600 0.45 +1.0 633,351 - ybdL b0600 0.47 +1.0 633,418 + ybdL b0600 0.52 +1.6 633,418 - ybdL b0600 0.52 +1.0 633,418 - ybdL b0600 0.52 -0.9 633,442 - ybdL b0600 0.55 +0.7 633,442 - ybdL b0600 0.55 -0.7 633,541 - ybdL b0600 0.63 -1.8 633,551 - ybdL b0600 0.64 +0.0 633,570 - ybdL b0600 0.66 +0.6 633,572 + ybdL b0600 0.66 -0.7 633,587 + ybdL b0600 0.67 -0.1 633,597 - ybdL b0600 0.68 -0.6 633,717 - ybdL b0600 0.78 -1.0 633,825 + ybdL b0600 0.88 -0.6 633,853 - ybdL b0600 0.90 -0.0 633,970 + +1.2 634,194 + ybdM b0601 0.36 +1.7 634,206 + ybdM b0601 0.37 -0.9 634,214 - ybdM b0601 0.39 -0.2 634,214 - ybdM b0601 0.39 -1.8 634,214 - ybdM b0601 0.39 -1.4 634,214 - ybdM b0601 0.39 -0.6 634,214 - ybdM b0601 0.39 -1.3 634,309 + ybdM b0601 0.54 -1.2 634,335 - ybdM b0601 0.58 -0.3 634,335 - ybdM b0601 0.58 +0.0 634,337 - ybdM b0601 0.58 +0.2 634,464 - ybdM b0601 0.78 -0.8 634,477 - ybdM b0601 0.80 +0.1 634,486 - ybdM b0601 0.82 -0.2 634,486 - ybdM b0601 0.82 +1.7 634,593 + -0.1 634,672 + -1.3 634,687 + +1.0 634,720 - ybdN b0602 0.12 +0.2 634,963 + ybdN b0602 0.32 +0.3
Or see this region's nucleotide sequence