Experiment: 2-Furfuraldehyde 0.125 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliD and fliS are separated by 24 nucleotides fliS and fliT overlap by 1 nucleotides fliT and amyA are separated by 77 nucleotides
b1924: fliD - flagellar capping protein (NCBI), at 2,001,896 to 2,003,302
fliD
b1925: fliS - flagellar protein FliS (NCBI), at 2,003,327 to 2,003,737
fliS
b1926: fliT - predicted chaperone (NCBI), at 2,003,737 to 2,004,102
fliT
b1927: amyA - cytoplasmic alpha-amylase (NCBI), at 2,004,180 to 2,005,667
amyA
Position (kb)
2003
2004
2005 Strain fitness (log2 ratio)
-1
0
1
2 at 2002.829 kb on - strand, within fliD at 2002.892 kb on - strand, within fliD at 2002.894 kb on - strand, within fliD at 2002.894 kb on - strand, within fliD at 2002.913 kb on - strand, within fliD at 2003.080 kb on - strand, within fliD at 2003.414 kb on + strand, within fliS at 2003.451 kb on - strand, within fliS at 2003.451 kb on - strand, within fliS at 2003.451 kb on - strand, within fliS at 2003.462 kb on + strand, within fliS at 2003.544 kb on + strand, within fliS at 2003.544 kb on + strand, within fliS at 2003.699 kb on + strand at 2003.734 kb on + strand at 2003.735 kb on - strand at 2003.738 kb on - strand at 2003.923 kb on - strand, within fliT at 2003.933 kb on - strand, within fliT at 2004.035 kb on + strand, within fliT at 2004.073 kb on + strand at 2004.135 kb on + strand at 2004.148 kb on + strand at 2004.241 kb on + strand at 2004.241 kb on + strand at 2004.249 kb on - strand at 2004.249 kb on - strand at 2004.249 kb on - strand at 2004.299 kb on + strand at 2004.446 kb on + strand, within amyA at 2004.461 kb on - strand, within amyA at 2004.533 kb on + strand, within amyA at 2004.543 kb on - strand, within amyA at 2004.614 kb on - strand, within amyA at 2004.614 kb on - strand, within amyA at 2004.739 kb on - strand, within amyA at 2004.809 kb on + strand, within amyA at 2004.861 kb on - strand, within amyA at 2004.942 kb on - strand, within amyA at 2005.017 kb on - strand, within amyA
Per-strain Table
Position Strand Gene LocusTag Fraction 2-Furfuraldehyde 0.125 vol% remove 2,002,829 - fliD b1924 0.66 +0.4 2,002,892 - fliD b1924 0.71 -0.5 2,002,894 - fliD b1924 0.71 -0.5 2,002,894 - fliD b1924 0.71 -0.0 2,002,913 - fliD b1924 0.72 -0.7 2,003,080 - fliD b1924 0.84 -0.2 2,003,414 + fliS b1925 0.21 +0.5 2,003,451 - fliS b1925 0.30 +0.1 2,003,451 - fliS b1925 0.30 -0.9 2,003,451 - fliS b1925 0.30 +0.5 2,003,462 + fliS b1925 0.33 +1.3 2,003,544 + fliS b1925 0.53 -0.7 2,003,544 + fliS b1925 0.53 +2.1 2,003,699 + +1.2 2,003,734 + +0.4 2,003,735 - +1.2 2,003,738 - -0.3 2,003,923 - fliT b1926 0.51 -0.8 2,003,933 - fliT b1926 0.54 -1.3 2,004,035 + fliT b1926 0.81 -0.3 2,004,073 + +1.0 2,004,135 + +0.3 2,004,148 + -0.2 2,004,241 + -0.2 2,004,241 + +0.1 2,004,249 - -0.2 2,004,249 - -0.5 2,004,249 - +1.0 2,004,299 + +0.3 2,004,446 + amyA b1927 0.18 +0.9 2,004,461 - amyA b1927 0.19 +0.6 2,004,533 + amyA b1927 0.24 -0.3 2,004,543 - amyA b1927 0.24 -0.5 2,004,614 - amyA b1927 0.29 +1.0 2,004,614 - amyA b1927 0.29 +0.5 2,004,739 - amyA b1927 0.38 -0.1 2,004,809 + amyA b1927 0.42 -0.2 2,004,861 - amyA b1927 0.46 +0.5 2,004,942 - amyA b1927 0.51 +0.4 2,005,017 - amyA b1927 0.56 +0.9
Or see this region's nucleotide sequence